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08-04-2023 | Ovariectomy | Original Article

Resveratrol prevents ovariectomy-induced bone quality deterioration by improving the microarchitectural and biophysicochemical properties of bone

Authors: Sevval Ozturk, Ibrahim Cuneyit, Fatih Altuntas, Ege Riza Karagur, Aysegul Cort Donmez, Mert Ocak, Mustafa Unal, Mehmet Sarikanat, Baris Ozgur Donmez

Published in: Journal of Bone and Mineral Metabolism | Issue 4/2023

Abstract

Introduction

Osteoporosis is a major health problem that is very common worldwide and is characterized by both low bone density and deterioration in bone quality. New treatment options without side effects have become an active area of research in recent years. This study was designed to investigate the preventive effects of resveratrol on bone quality deterioration caused by ovariectomy.

Materials and Methods

Sixty rats were randomly divided into five groups (12 animals per group): Control, Sham-operated (SHAM), ovariectomized (OVX), OVX + Resveratrol-40 mg/kg/day (OVX + Res40), OVX + Resveratrol-80 mg/kg/day (OVX + Res80). Resveratrol was administered by oral gavage (40 and 80 mg/kg/day) for ten weeks. Micro-CT measurements, biomechanical testing, Raman spectroscopy analysis, and RT-PCR analysis were performed. ALP, OCN, TAS, and TOS levels were also measured from blood serum.

Results

Bone strength, bone volume/total volume, trabecular volume, and trabecular thickness were higher in the OVX + RES-80 group than in the OVX group. Resveratrol increased osteogenic differentiation, as the expression of osteogenic markers ALP, Col1A1, Runx2, OPG, OCN increased in both OVX + RES-80 and OVX + RES-40 groups compared to the OVX group. 80 mg/kg/day resveratrol administration decreased the levels of ALP, OCN and TOS in ovariectomized rats. Raman spectroscopy findings showed a preventive effect of resveratrol administration against ovariectomy-induced deterioration in biophysiochemical properties of bone tissue.

Conclusion

This study revealed that administration of different doses of 80 mg/kg/day and 40 mg/kg/day of resveratrol had protective effects on bone quality deterioration caused by ovariectomy.

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Title: Resveratrol prevents ovariectomy-induced bone quality deterioration by improving the microarchitectural and biophysicochemical properties of bone
Authors: Sevval Ozturk
Ibrahim Cuneyit
Fatih Altuntas
Ege Riza Karagur
Aysegul Cort Donmez
Mert Ocak
Mustafa Unal
Mehmet Sarikanat
Baris Ozgur Donmez
Publication date: 08-04-2023
Publisher: Springer Nature Singapore
Keywords: Ovariectomy
Osteoporosis
Osteoporosis
Published in: Journal of Bone and Mineral Metabolism / Issue 4/2023
Print ISSN: 0914-8779
Electronic ISSN: 1435-5604
DOI: https://doi.org/10.1007/s00774-023-01416-z

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