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Published in: Journal of Assisted Reproduction and Genetics 7/2008

01-07-2008 | Case Report

Oocyte cryopreservation: the birth of the first Hungarian babies from frozen oocytes

Authors: Janos Konc, Katalin Kanyo, Erika Varga, Rita Kriston, Sandor Cseh

Published in: Journal of Assisted Reproduction and Genetics | Issue 7/2008

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Abstract

Purpose

To present data obtained with clinical application of oocyte cryopreservation.

Methods

Slow freezing/rapid thawing in PBS based medium containing 1.5 M propanediol + 0.3 M sucrose.

Results

A total of 127 embryos were transferred into 54 patients (1.9 embryo/cycle, 64 transfer cycles). Clinical pregnancy rate of 20% per cycle (13/64) and 24.0% per patient were achieved. Up-to-date, six patients delivered seven healthy babies; there are four ongoing pregnancies. Three abortions (23%) and one biochemical pregnancy (0.7%) was obtained. Implantation rates of 11% per transferred embryos (14/127) and 6.5% (14/215) per thawed eggs were found. In each case, normal karyotype was detected. No difference was found in the ratio of spindle positive oocytes at the polscope analyses done before and after freezing (75.8% vs. 82.5%).

Conclusion

Egg freezing is not a routine procedure yet, but there will certainly be a place for it in the future of assisted reproductive medicine.
Literature
1.
go back to reference Marina F, Marina S. Comments on oocyte cryopreservation. Reprod Biomed Online 2003;6:401–2.PubMed Marina F, Marina S. Comments on oocyte cryopreservation. Reprod Biomed Online 2003;6:401–2.PubMed
2.
go back to reference Chen SU, Lien YR, Chen HF, Chang LJ, Tsai YY, Yang YS. Observational clinical follow-up of oocyte cryopreservation using a slow-freezing method with 1,2-propanediol plus sucrose followed by ICSI. Hum Reprod 2005;20:1975–80. doi:10.1093/humrep/deh884.PubMedCrossRef Chen SU, Lien YR, Chen HF, Chang LJ, Tsai YY, Yang YS. Observational clinical follow-up of oocyte cryopreservation using a slow-freezing method with 1,2-propanediol plus sucrose followed by ICSI. Hum Reprod 2005;20:1975–80. doi:10.​1093/​humrep/​deh884.PubMedCrossRef
3.
go back to reference Borini A, Cottichio G, Flamigni C. Oocyte freezing: a positive comment based on our experience. Reprod Biomed Online 2003;7:120.PubMed Borini A, Cottichio G, Flamigni C. Oocyte freezing: a positive comment based on our experience. Reprod Biomed Online 2003;7:120.PubMed
5.
go back to reference Stachecki JJ, Cohen J. An overview of oocyte cryopreservation. Reprod Biomed Online 2004;9(2):152–63.PubMed Stachecki JJ, Cohen J. An overview of oocyte cryopreservation. Reprod Biomed Online 2004;9(2):152–63.PubMed
6.
go back to reference Stachecki JJ, Cohen J, Garrisi J, Munne S, Burgess C, Willadsen SM. Cryopreservation of unfertilized human oocytes. Reprod Biomed Online 2006;13:222–7.PubMedCrossRef Stachecki JJ, Cohen J, Garrisi J, Munne S, Burgess C, Willadsen SM. Cryopreservation of unfertilized human oocytes. Reprod Biomed Online 2006;13:222–7.PubMedCrossRef
7.
go back to reference Tucker MJ, Shipley S, Liebermann J. Conventional technologies for cryopreservation of human oocytes and embryos. Methods Mol Biol 2004;254:325–44.PubMed Tucker MJ, Shipley S, Liebermann J. Conventional technologies for cryopreservation of human oocytes and embryos. Methods Mol Biol 2004;254:325–44.PubMed
8.
go back to reference Borini A, Lagalla C, Bonu MA. Cumulative pregnancy rates resulting from the use of fresh and frozen oocytes: 7 years’ experience. Reprod Biomed Online 2006;12:481–6.PubMedCrossRef Borini A, Lagalla C, Bonu MA. Cumulative pregnancy rates resulting from the use of fresh and frozen oocytes: 7 years’ experience. Reprod Biomed Online 2006;12:481–6.PubMedCrossRef
14.
go back to reference Boiso I, Marti M, Santalo J, Marti M, Santalo J, Ponsa M, et al. A confocal microscopy analysis of the spindle and chromosomese configuration of human oocytes cryopreserved at the germinal vesicle and metaphase II stage. Hum Reprod 2002;17:1885–91. doi:10.1093/humrep/17.7.1885.PubMedCrossRef Boiso I, Marti M, Santalo J, Marti M, Santalo J, Ponsa M, et al. A confocal microscopy analysis of the spindle and chromosomese configuration of human oocytes cryopreserved at the germinal vesicle and metaphase II stage. Hum Reprod 2002;17:1885–91. doi:10.​1093/​humrep/​17.​7.​1885.PubMedCrossRef
15.
go back to reference Gook DA, Osborn SM, Johnston WI. Cryopreservation of mouse and human oocytes using 1,2-propanediol and the configuration of the meiotic spindle. Hum Reprod 1993;8:1101–9.PubMed Gook DA, Osborn SM, Johnston WI. Cryopreservation of mouse and human oocytes using 1,2-propanediol and the configuration of the meiotic spindle. Hum Reprod 1993;8:1101–9.PubMed
16.
go back to reference Rienzi L, Matinez F, Ubaldi F, Minasi MG, Iacobelli M, Tesarik J, et al. Polscope analysis of meiotic spindle changes in living metaphase II human oocytes during the freezing and thawing procedures. Hum Reprod 2004;19:655–9. doi:10.1093/humrep/deh101.PubMedCrossRef Rienzi L, Matinez F, Ubaldi F, Minasi MG, Iacobelli M, Tesarik J, et al. Polscope analysis of meiotic spindle changes in living metaphase II human oocytes during the freezing and thawing procedures. Hum Reprod 2004;19:655–9. doi:10.​1093/​humrep/​deh101.PubMedCrossRef
17.
18.
go back to reference Gook DA, Osborn SM, Bourne H, Johnston WI. Fertilization of human oocytes following cryopreservation; normal karyotypes and absence of stray chromosomes. Hum Reprod 1994;9:684–91.PubMed Gook DA, Osborn SM, Bourne H, Johnston WI. Fertilization of human oocytes following cryopreservation; normal karyotypes and absence of stray chromosomes. Hum Reprod 1994;9:684–91.PubMed
20.
go back to reference Hellebaut S, De Sutter P, Dozortsev D, Onghena A, Qian C, Dhont M. Does assisted hatching improve implantation rates after in vitro fertilization or intracytoplasmic sperm injection in all patients? A prospective randomized study. J Assist Reprod Genet 1996;13:19–22.PubMedCrossRef Hellebaut S, De Sutter P, Dozortsev D, Onghena A, Qian C, Dhont M. Does assisted hatching improve implantation rates after in vitro fertilization or intracytoplasmic sperm injection in all patients? A prospective randomized study. J Assist Reprod Genet 1996;13:19–22.PubMedCrossRef
Metadata
Title
Oocyte cryopreservation: the birth of the first Hungarian babies from frozen oocytes
Authors
Janos Konc
Katalin Kanyo
Erika Varga
Rita Kriston
Sandor Cseh
Publication date
01-07-2008
Publisher
Springer US
Published in
Journal of Assisted Reproduction and Genetics / Issue 7/2008
Print ISSN: 1058-0468
Electronic ISSN: 1573-7330
DOI
https://doi.org/10.1007/s10815-008-9235-0

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