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BMC Complementary Medicine and Therapies
Published in: BMC Complementary Medicine and Therapies 1/2019

Open Access 01-12-2019 | NSCLC | Research article

Jingfukang induces anti-cancer activity through oxidative stress-mediated DNA damage in circulating human lung cancer cells

Authors: Zujun Que, Zhiyi Zhou, Bin Luo, Changsheng Dong, Yi Jiang, Hegen Li, Jianhui Tian

Published in: BMC Complementary Medicine and Therapies | Issue 1/2019

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Abstract

Background

Metastasis is the main cause of lung cancer death. As a seed of metastasis, circulating tumor cells are an important target for metastasis intervention. The traditional Chinese medicine, Jinfukang, has been clinically available for the treatment of non-small cell lung cancer (NSCLC). In this study, we investigated the action and underlying mechanisms of Jinfukang against circulating lung tumor cells.

Methods

The cell counting kit-8 (CCK-8), colony formation and cell cycle assays were used to study the cell proliferation ability. Flow cytometry was used to detect the apoptosis and the expression level of ROS and Caspase-3. Comet and TUNEL assays were used to detect DNA damage. DNA damage related pathway protein was detected by western blot.

Results

Jinfukang significantly inhibits the proliferation of CTC-TJH-01 cells by inducing G1 phase arrest and inhibits their colony formation in a dose-dependent manner. Moreover, Jinfukang induces apoptosis in CTC-TJH-01 cells through the ROS-mediated ATM/ATR-p53 pathway and DNA damage.

Conclusions

Our findings suggest that Jinfukang may be a potential drug for lung cancer metastasis.
Appendix
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Metadata
Title
Jingfukang induces anti-cancer activity through oxidative stress-mediated DNA damage in circulating human lung cancer cells
Authors
Zujun Que
Zhiyi Zhou
Bin Luo
Changsheng Dong
Yi Jiang
Hegen Li
Jianhui Tian
Publication date
01-12-2019
Publisher
BioMed Central
Published in
BMC Complementary Medicine and Therapies / Issue 1/2019
Electronic ISSN: 2662-7671
DOI
https://doi.org/10.1186/s12906-019-2601-x

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