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Archives of Virology
Published in: Archives of Virology 1/2009

01-01-2009 | Brief Report

Novel virus discovery in field-collected mosquito larvae using an improved system for rapid determination of viral RNA sequences (RDV ver4.0)

Authors: Takuya Yamao, Yuki Eshita, Yuki Kihara, Tomomitsu Satho, Makoto Kuroda, Tsuyoshi Sekizuka, Miho Nishimura, Kouji Sakai, Shumpei Watanabe, Hiroomi Akashi, Yupha Rongsriyam, Narumon Komalamisra, Raweewan Srisawat, Takeshi Miyata, Akira Sakata, Masato Hosokawa, Manabu Nakashima, Nobuhiro Kashige, Fumio Miake, Shuetsu Fukushi, Mina Nakauchi, Masayuki Saijo, Ichiro Kurane, Shigeru Morikawa, Tetsuya Mizutani

Published in: Archives of Virology | Issue 1/2009

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Abstract

In this study, we improved a method for rapid determination of viral RNA sequences (RDV) to overcome the limitations of previous versions. The RDV ver4.0 method can detect RNA sequences with at least 1,000 copies as starting material. A novel virus, which was isolated from field-collected Aedes aegypti larvae in the Phasi Charoen district of Thailand using C6/36 cells, was identified using the RDV ver4.0 protocol. The virus was named Phasi Charoen virus (PhaV). We used a high-throughput pyrosequencing approach to obtain more information about the genome sequence of PhaV. Analysis of a phylogenic tree based on amino acid sequences strongly suggested that PhaV belongs to the family Bunyaviridae.
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Metadata
Title
Novel virus discovery in field-collected mosquito larvae using an improved system for rapid determination of viral RNA sequences (RDV ver4.0)
Authors
Takuya Yamao
Yuki Eshita
Yuki Kihara
Tomomitsu Satho
Makoto Kuroda
Tsuyoshi Sekizuka
Miho Nishimura
Kouji Sakai
Shumpei Watanabe
Hiroomi Akashi
Yupha Rongsriyam
Narumon Komalamisra
Raweewan Srisawat
Takeshi Miyata
Akira Sakata
Masato Hosokawa
Manabu Nakashima
Nobuhiro Kashige
Fumio Miake
Shuetsu Fukushi
Mina Nakauchi
Masayuki Saijo
Ichiro Kurane
Shigeru Morikawa
Tetsuya Mizutani
Publication date
01-01-2009
Publisher
Springer Vienna
Published in
Archives of Virology / Issue 1/2009
Print ISSN: 0304-8608
Electronic ISSN: 1432-8798
DOI
https://doi.org/10.1007/s00705-008-0285-5

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