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Archives of Virology
Published in: Archives of Virology 1/2009

01-01-2009 | Brief Report

Simultaneous detection of human enterovirus 71 and coxsackievirus A16 in clinical specimens by multiplex real-time PCR with an internal amplification control

Authors: Xing-Long Xiao, Ya-Qing He, Yi-Gang Yu, Hong Yang, Gu Chen, Hui-Fang Li, Jing-Wei Zhang, Dong-Mei Liu, Xiao-Feng Li, Xiao-Quan Yang, Hui Wu

Published in: Archives of Virology | Issue 1/2009

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Abstract

The recent and continuing HFMD outbreak caused by EV71 in several provinces of China since March 2008 has affected thousands of children and resulted in nearly 50 deaths. In this study, a sensitive and specific multiplex real-time RT-PCR assay has been developed for the rapid detection of EV71 and CV-A16. By using an internal amplification control, the real-time assay achieves detection of samples containing inhibitors and avoids false negatives. It should prove useful for clinical diagnosis of EV71 or CV-A16 infections.
Literature
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Cardosa MJ, Perera D, Brown BA et al (2003) Molecular epidemiology of human enterovirus 71 strains and recent outbreaks in the Asia-Pacific region: comparative analysis of the VP1 and VP4 genes. Emerg Infect Dis 9:461–468PubMed
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Lin TY, Chang LY, Hsia SH et al (2002) The 1998 enterovirus 71 outbreak in Taiwan: pathogenesis and management. Clin Infect Dis 34(Suppl 2):S52–S57PubMedCrossRef
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Tan EL, Chow VT, Quak SH et al (2008) Development of multiplex real-time hybridization probe reverse transcriptase polymerase chain reaction for specific detection and differentiation of Enterovirus 71 and Coxsackievirus A16. Diagn Microbiol Infect Dis (in press)
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Rosenstraus M, Wang Z, Chang SY et al (1998) An internal control for routine diagnostic pcr: design, properties, and effect on clinical performance. J Clin Microbiol 36:191–197PubMed
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Ooi MH, Solomon T, Podin Y et al (2007) Evaluation of different clinical sample types in diagnosis of human enterovirus 71-associated hand-foot-and-mouth disease. J Clin Microbiol 45:1858–1866PubMedCrossRef
Metadata
Title
Simultaneous detection of human enterovirus 71 and coxsackievirus A16 in clinical specimens by multiplex real-time PCR with an internal amplification control
Authors
Xing-Long Xiao
Ya-Qing He
Yi-Gang Yu
Hong Yang
Gu Chen
Hui-Fang Li
Jing-Wei Zhang
Dong-Mei Liu
Xiao-Feng Li
Xiao-Quan Yang
Hui Wu
Publication date
01-01-2009
Publisher
Springer Vienna
Published in
Archives of Virology / Issue 1/2009
Print ISSN: 0304-8608
Electronic ISSN: 1432-8798
DOI
https://doi.org/10.1007/s00705-008-0266-8

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