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Open Access 01-12-2023 | Nitrate | Study protocol

Nitrate-rich beet juice intake on cardiovascular performance in response to exercise in postmenopausal women with arterial hypertension: study protocol for a randomized controlled trial

Authors: Cicero Jonas R. Benjamim, Yaritza Brito Alves Sousa, Andrey Alves Porto, Yasmim Mota de Moraes Pontes, Simone Sakagute Tavares, Guilherme da Silva Rodrigues, Leonardo Santos Lopes da Silva, Leonardo da Silva Goncalves, Carolina Scoqui Guimaraes, Macário Arosti Rebelo, Andressa Crystine da Silva Sobrinho, Jose E. Tanus-Santos, Vitor Engracia Valenti, Bruno Gualano, Carlos Roberto Bueno Júnior

Published in: Trials | Issue 1/2023

Abstract

Background

There is no evidence of the use of beetroot juice with a previously recommended dose of nitrate (NO3) (> 300 mg) on the cardiovascular performance during and recovery following exercise in postmenopausal women with systemic arterial hypertension (SAH).

Methods

We will investigate the effects of beetroot juice rich in NO3 acutely (800 mg) and during a week with daily doses (400 mg) on blood pressure, heart rate (HR), cardiac autonomic control, endothelial function, inflammatory, hormonal, and stress biomarkers oxidative stress and enzymes involved in nitric oxide synthesis and mitochondrial regulation, under resting conditions, as well as mediated by submaximal aerobic exercise sessions. Through a randomized, crossover, triple-blind, placebo-controlled clinical trial, 25 physically inactive women with SAH will undergo an acute and 1-week trial, each with two intervention protocols: (1) placebo and (2) beetroot, in which will ingest beet juice with or without NO3 in its composition with a 7-day washout interval. On collection days, exercise will be performed on a treadmill for 40 min at a speed corresponding to 65–70% of VO2peak. The collection of variables (cardiovascular, autonomic, and blood samples for molecular analyses) of the study will take place at rest (135 min after ingestion of the intervention), during exercise (40 min), and in the effort recovery stage (during 60 min) based on previously validated protocols. The collections were arranged so that the measurement of one variable does not interfere with the other and that they have adequate intervals between them.

Discussion

The results of this research may help in the real understanding of the nutritional compounds capable of generating safety to the cardiovascular system during physical exercise, especially for women who are aging and who have cardiovascular limitations (e.g., arterial hypertension) to perform physical exercise. Therefore, our results will be able to help specific nutritional recommendations to optimize cardiovascular health.

Trial registration

ClinicalTrials.gov NCT05384340. Registered on May 20, 2022.

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Title: Nitrate-rich beet juice intake on cardiovascular performance in response to exercise in postmenopausal women with arterial hypertension: study protocol for a randomized controlled trial
Authors: Cicero Jonas R. Benjamim
Yaritza Brito Alves Sousa
Andrey Alves Porto
Yasmim Mota de Moraes Pontes
Simone Sakagute Tavares
Guilherme da Silva Rodrigues
Leonardo Santos Lopes da Silva
Leonardo da Silva Goncalves
Carolina Scoqui Guimaraes
Macário Arosti Rebelo
Andressa Crystine da Silva Sobrinho
Jose E. Tanus-Santos
Vitor Engracia Valenti
Bruno Gualano
Carlos Roberto Bueno Júnior
Publication date: 01-12-2023
Publisher: BioMed Central
Keywords: Nitrate
Hypertension
Arterial Hypertension
Arterial Hypertension
Published in: Trials / Issue 1/2023
Electronic ISSN: 1745-6215
DOI: https://doi.org/10.1186/s13063-023-07117-2

At a glance: The STEP trials

Springer Medicine

Nitrate-rich beet juice intake on cardiovascular performance in response to exercise in postmenopausal women with arterial hypertension: study protocol for a randomized controlled trial

Abstract

Background

Methods

Discussion

Trial registration

At a glance: The STEP trials

Springer Medicine

Abstract

Background

Methods

Discussion

Trial registration

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