Top

Published in:

04-04-2023 | Neuropathic Pain | Original Article

Stem cell therapy combined with luteolin alleviates experimental neuropathy

Authors: Sajad Sahab Negah, Mehrdad Hajinejad, Saeideh Nemati, Seyyed Mohamad Javad Mahdipour Roudbary, Fatemeh Forouzanfar

Published in: Metabolic Brain Disease | Issue 6/2023

Abstract

Neuropathic pain is a chronic condition that causes long-term burning sensations. Despite significant efforts, current treatments for neuropathic pain are ineffective in curing the condition, which means new therapeutic options must be developed. One such option is the use of stem cell therapy in combination with anti-inflammatory herbal components, which has shown promise in treating neuropathic pain. The study aimed to investigate the effects of bone marrow mesenchymal stem cells (BM-MSCs) with luteolin on sensory deficits and pathological changes in a neuropathic model. The results showed that luteolin, either alone or in combination with BM-MSCs, effectively reduced sensory deficits related to mechanical and thermal hypersensitivity. In addition, luteolin alone and combined with BM-MSCs reduced oxidative stress in neuropathic rats and inhibited cellular responses, particularly reactive astrocytes. The study concluded that combining luteolin and BM-MSCs may offer an effective therapeutic strategy for patients with neuropathic pain, although further research is needed.

Asgharzade S, Talaei A, Farkhondeh T, Forouzanfar F (2020) A review on stem cell therapy for neuropathic pain. Curr Stem Cell Res Ther 15:349–361CrossRefPubMed

Austin PJ, Moalem-Taylor G (2010) The neuro-immune balance in neuropathic pain: involvement of inflammatory immune cells, immune-like glial cells and cytokines. J Neuroimmunol 229:26–50CrossRefPubMed

Bennett GJ, Xie Y-K (1988) A peripheral mononeuropathy in rat that produces disorders of pain sensation like those seen in man. Pain 33:87–107CrossRefPubMed

Bernetti A et al (2021) Neuropathic pain and rehabilitation: a systematic review of international guidelines. Diagnostics 11:74CrossRefPubMedPubMedCentral

Calió ML et al (2014) Transplantation of bone marrow mesenchymal stem cells decreases oxidative stress, apoptosis, and hippocampal damage in brain of a spontaneous stroke model. Free Radic Biol Med 70:141–154CrossRefPubMed

Chen G, Park C-K, Xie R-G, Ji R-R (2015) Intrathecal bone marrow stromal cells inhibit neuropathic pain via TGF-β secretion. J Clin Invest 125:3226–3240CrossRefPubMedPubMedCentral

Dirscherl K et al (2010) Luteolin triggers global changes in the microglial transcriptome leading to a unique anti-inflammatory and neuroprotective phenotype. J Neuroinflammation 7:1–16CrossRef

Forouzanfar F, Hosseinzadeh H, Khorrami MB, Asgharzade S, Rakhshandeh H (2019) Attenuating Effect of Portulaca oleracea Extract on Chronic Constriction Injury Induced Neuropathic Pain in Rats: An Evidence of Antioxidative and Anti-inflammatory Effects. CNS Neurol Disord Drug Targets 18:342–349CrossRefPubMed

Hara K, Haranishi Y, Terada T, Takahashi Y, Nakamura M, Sata T (2014) Effects of intrathecal and intracerebroventricular administration of luteolin in a rat neuropathic pain model. Pharmacol Biochem Behav 125:78–84CrossRefPubMed

Kamishina H, Deng J, Oji T, Cheeseman JA, Clemmons RM (2006) Expression of neural markers on bone marrow–derived canine mesenchymal stem cells. Am J Vet Res 67:1921–1928CrossRefPubMed

Kempuraj D et al (2021) Neuroprotective effects of flavone luteolin in neuroinflammation and neurotrauma. Biofactors 47:190–197CrossRefPubMed

Klopp AH, Gupta A, Spaeth E, Andreeff M, Marini F III (2011) Concise review: dissecting a discrepancy in the literature: do mesenchymal stem cells support or suppress tumor growth? Stem Cells 29:11–19CrossRefPubMed

Li M, Li Q, Zhao Q, Zhang J, Lin J (2015) Luteolin improves the impaired nerve functions in diabetic neuropathy: behavioral and biochemical evidences. Int J Clin Exp Pathol 8:10112PubMedPubMedCentral

Mannelli LDC, Cinci L, Micheli L, Zanardelli M, Pacini A, McIntosh JM, Ghelardini C (2014) α-Conotoxin RgIA protects against the development of nerve injury-induced chronic pain and prevents both neuronal and glial derangement. Pain® 155:1986–1995CrossRef

Miao C, Lei M, Hu W, Han S, Wang Q (2017) A brief review: the therapeutic potential of bone marrow mesenchymal stem cells in myocardial infarction. Stem Cell Res Ther 8:1–6CrossRef

Nabavi SF, Braidy N, Gortzi O, Sobarzo-Sanchez E, Daglia M, Skalicka-Woźniak K, Nabavi SM (2015) Luteolin as an anti-inflammatory and neuroprotective agent: a brief review. Brain Res Bull 119:1–11CrossRefPubMed

Oggu GS, Sasikumar S, Reddy N, Ella KKR, Rao CM, Bokara KK (2017) Gene delivery approaches for mesenchymal stem cell therapy: strategies to increase efficiency and specificity. Stem Cell Rev Rep 13:725–740CrossRefPubMed

Rakhshandeh H, Asgharzade S, Khorrami MB, Forouzanfar F (2021a) Protective effect of Capparis spinosa extract against focal cerebral ischemia-reperfusion. Injury in Rats Cent Nerv Syst Agents Med Chem 21:148–153. https://doi.org/10.2174/1871524921666210625112356CrossRefPubMed

Rakhshandeh H, Ghorbanzadeh A, Negah SS, Akaberi M, Rashidi R, Forouzanfar F (2021b) Pain-relieving effects of Lawsonia inermis on neuropathic pain induced by chronic constriction injury. Metab Brain Dis 36:1709–1716CrossRefPubMed

Rakhshandeh H, Pourbagher-Shahri AM, Hasanpour M, Iranshahi M, Forouzanfar F (2022) Effects of Capparis Spinosa extract on the neuropathic pain induced by chronic constriction injury in rats. Metabolic Brain Disease:1–14

Shehab SAS (2009) Acute and chronic sectioning of fifth lumbar spinal nerve has equivalent effects on the primary afferents of sciatic nerve in rat spinal cord. J Comp Neurol 517:481–492CrossRefPubMed

Siniscalco D, Giordano C, Galderisi U, Luongo L, de Novellis V, Rossi F, Maione S (2011) Long-lasting effects of human mesenchymal stem cell systemic administration on pain-like behaviors, cellular, and biomolecular modifications in neuropathic mice. Front Integr Neurosci 5:79CrossRefPubMedPubMedCentral

Sun T et al (2015) Bone marrow-derived mesenchymal stem cell transplantation ameliorates oxidative stress and restores intestinal mucosal permeability in chemically induced colitis in mice. Am J Transl Res 7:891–901PubMedPubMedCentral

Swaminathan A, Basu M, Bekri A, Drapeau P, Kundu TK (2019) The dietary flavonoid, luteolin, negatively affects neuronal differentiation. Front Mol Neurosci 12:41CrossRefPubMedPubMedCentral

Tibbs GR, Posson DJ, Goldstein PA (2016) Voltage-gated ion channels in the PNS: novel therapies for neuropathic pain? Trends Pharmacol Sci 37:522–542CrossRefPubMed

Udalamaththa VL, Jayasinghe CD, Udagama PV (2016) Potential role of herbal remedies in stem cell therapy: proliferation and differentiation of human mesenchymal stromal cells. Stem Cell Res Ther 7:1–8CrossRef

Valle-Prieto A, Conget PA (2010) Human mesenchymal stem cells efficiently manage oxidative stress. Stem Cells Dev 19:1885–1893CrossRefPubMed

Vivoli E et al (2010) Acetyl-L-carnitine increases artemin level and prevents neurotrophic factor alterations during neuropathy. Neuroscience 167:1168–1174CrossRefPubMed

Wexler SA, Donaldson C, Denning-Kendall P, Rice C, Bradley B, Hows JM (2003) Adult bone marrow is a rich source of human mesenchymal ‘stem’cells but umbilical cord and mobilized adult blood are not. Br J Haematol 121:368–374CrossRefPubMed

Yang Y et al (2020) Luteolin alleviates neuroinflammation via downregulating the TLR4/TRAF6/NF-κB pathway after intracerebral hemorrhage biomedicine &. Pharmacotherapy 126:110044CrossRef

Yousefifard M, Nasirinezhad F, Manaheji HS, Janzadeh A, Hosseini M, Keshavarz M (2016) Human bone marrow-derived and umbilical cord-derived mesenchymal stem cells for alleviating neuropathic pain in a spinal cord injury model. Stem Cell Res Ther 7:1–14CrossRef

Title: Stem cell therapy combined with luteolin alleviates experimental neuropathy
Authors: Sajad Sahab Negah
Mehrdad Hajinejad
Saeideh Nemati
Seyyed Mohamad Javad Mahdipour Roudbary
Fatemeh Forouzanfar
Publication date: 04-04-2023
Publisher: Springer US
Keyword: Neuropathic Pain
Published in: Metabolic Brain Disease / Issue 6/2023
Print ISSN: 0885-7490
Electronic ISSN: 1573-7365
DOI: https://doi.org/10.1007/s11011-023-01206-6

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Stem cell therapy combined with luteolin alleviates experimental neuropathy

Abstract

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

Please log in to get access to this content

Other articles of this Issue 6/2023

Paraoxonase 1 status is a major Janus-faced component of mild and moderate acute ischemic stroke and consequent disabilities

The network pharmacology study and molecular docking to investigate the potential mechanism of Acoritataninowii Rhizoma against Alzheimer's Disease

CircZfp609 contributes to cerebral infarction via sponging miR-145a-5p to regulate BACH1

miR-181a targets PTEN to mediate the neuronal injury caused by oxygen-glucose deprivation and reoxygenation

3-Methyladenine ameliorates surgery-induced anxiety-like behaviors in aged mice by inhibiting autophagy-induced excessive oxidative stress

Strategies for enhancing beneficial effects of selenium on cognitive function