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Journal of Assisted Reproduction and Genetics

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01-11-2014 | Gamete Biology

Morphologic and proliferative characteristics of goat type a spermatogonia in the presence of different sets of growth factors

Authors: Mohammad Sadra Shirazi, Banafsheh Heidari, Abolfazl Shirazi, Amir Hassan Zarnani, Mahmood Jeddi-Tehrani, Maryam Rahmati-Ahmadabadi, Mohammad Mehdi Naderi, Bahareh Behzadi, Moretza Farab, Ali Sarvari, Sara Borjian-Boroujeni, Mohammad Mehdi Akhondi

Published in: Journal of Assisted Reproduction and Genetics | Issue 11/2014

Abstract

Purpose

The present study by using different growth factors was aimed to develop the best practical culture condition for purification of goat undifferentiated SSCs and their colonization under in vitro and in vivo conditions.

Methods

The enzymatically isolated SSCs obtained from one month old goat testes were cultured in DMEM plus FCS supplemented with different sets of growth factors (GDNF, LIF, bFGF, and EGF) for 2 weeks. At the end of each week, the morphological characteristics of cells and colonies alongside with purification rate of undifferentiated type A spermatogonia were evaluated by immunocytochemical staining and flow cytometry.

Results

The number and size of colonies in treatment groups were significantly (P < 0.01) higher than corresponding values in control group. In immunocytochemical evaluation, the proportion of KIT and PGP9.5 positive cells were significantly (P < 0.001) higher in control and treatment groups, respectively.

Conclusions

The culture medium comprising all four growth factors, especially the one supplemented with the higher concentration of GDNF, was superior to the other groups with respect to the population of undifferentiated type A spermatogonia and its propagation in culture system. Additionally, goat SSCs could colonize within the mouse testis following xenotransplantation.

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Title: Morphologic and proliferative characteristics of goat type a spermatogonia in the presence of different sets of growth factors
Authors: Mohammad Sadra Shirazi
Banafsheh Heidari
Abolfazl Shirazi
Amir Hassan Zarnani
Mahmood Jeddi-Tehrani
Maryam Rahmati-Ahmadabadi
Mohammad Mehdi Naderi
Bahareh Behzadi
Moretza Farab
Ali Sarvari
Sara Borjian-Boroujeni
Mohammad Mehdi Akhondi
Publication date: 01-11-2014
Publisher: Springer US
Published in: Journal of Assisted Reproduction and Genetics / Issue 11/2014
Print ISSN: 1058-0468
Electronic ISSN: 1573-7330
DOI: https://doi.org/10.1007/s10815-014-0301-5

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Morphologic and proliferative characteristics of goat type a spermatogonia in the presence of different sets of growth factors

Abstract

Purpose

Methods

Results

Conclusions

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

Purpose

Methods

Results

Conclusions

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