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The Journal of Headache and Pain

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Open Access 01-12-2019 | Migraine | Research article

Electrical stimulation of the superior sagittal sinus suppresses A-type K⁺ currents and increases P/Q- and T-type Ca²⁺ currents in rat trigeminal ganglion neurons

Authors: Junping Cao, Yuan Zhang, Lei Wu, Lidong Shan, Yufang Sun, Xinghong Jiang, Jin Tao

Published in: The Journal of Headache and Pain | Issue 1/2019

Abstract

Background

Migraine is a debilitating neurological disorder involving abnormal trigeminovascular activation and sensitization. However, the underlying cellular and molecular mechanisms remain unclear.

Methods

A rat model of conscious migraine was established through the electrical stimulation (ES) of the dural mater surrounding the superior sagittal sinus. Using patch clamp recording, immunofluorescent labelling, enzyme-linked immunosorbent assays and western blot analysis, we studied the effects of ES on sensory neuronal excitability and elucidated the underlying mechanisms mediated by voltage-gated ion channels.

Results

The calcitonin gene-related peptide (CGRP) level in the jugular vein blood and the number of CGRP-positive neurons in the trigeminal ganglia (TGs) were significantly increased in rats with ES-induced migraine. The application of ES increased actional potential firing in both small-sized IB₄-negative (IB₄⁻) and IB₄⁺ TG neurons. No significant changes in voltage-gated Na⁺ currents were observed in the ES-treated groups. ES robustly suppressed the transient outward K⁺ current (I_A) in both types of TG neurons, while the delayed rectifier K⁺ current remained unchanged. Immunoblot analysis revealed that the protein expression of Kv4.3 was significantly decreased in the ES-treated groups, while Kv1.4 remained unaffected. Interestingly, ES increased the P/Q-type and T-type Ca²⁺ currents in small-sized IB₄⁻ TG neurons, while there were no significant changes in the IB₄⁺ subpopulation of neurons.

Conclusion

These results suggest that ES decreases the I_A in small-sized TG neurons and increases P/Q- and T-type Ca²⁺ currents in the IB₄⁻ subpopulation of TG neurons, which might contribute to neuronal hyperexcitability in a rat model of ES-induced migraine.

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Title: Electrical stimulation of the superior sagittal sinus suppresses A-type K+ currents and increases P/Q- and T-type Ca2+ currents in rat trigeminal ganglion neurons
Authors: Junping Cao
Yuan Zhang
Lei Wu
Lidong Shan
Yufang Sun
Xinghong Jiang
Jin Tao
Publication date: 01-12-2019
Publisher: Springer Milan
Keyword: Migraine
Published in: The Journal of Headache and Pain / Issue 1/2019
Print ISSN: 1129-2369
Electronic ISSN: 1129-2377
DOI: https://doi.org/10.1186/s10194-019-1037-5

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Electrical stimulation of the superior sagittal sinus suppresses A-type K⁺ currents and increases P/Q- and T-type Ca²⁺ currents in rat trigeminal ganglion neurons

Abstract

Background

Methods

Results

Conclusion

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

Background

Methods

Results

Conclusion

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