Skip to main content
Key Specialties
Cardiology Diabetology Endocrinology Gastroenterology Geriatrics and Gerontology Gynecology and Obstetrics Hematology Infectious Disease Internal Medicine Nephrology Neurology Oncology Pediatrics Respiratory Medicine Rheumatology Surgery
Congress Coverage
2024 ACC Congress 2023 ESMO Congress 2023 EASD Congress 2023 ERS Congress 2023 ESC Congress
Clinical Collections
Advances in Alzheimer’s

At a glance: The STEP trials

A round-up of the STEP phase 3 clinical trials evaluating semaglutide for weight loss in people with overweight or obesity.
ADVANCED SEARCH
Log in
Top
Virchows Archiv
Published in: Virchows Archiv 6/2012

01-12-2012 | Original Article

MicroRNA expression profiles of seminoma from paraffin-embedded formalin-fixed tissue

Authors: Z. Bing, S. R. Master, J. W. Tobias, D. A. Baldwin, X. W. Xu, J. E. Tomaszewski

Published in: Virchows Archiv | Issue 6/2012

Login to get access

Abstract

In this study, we used microRNA (miRNA) microarrays in an unbiased screen for aberrantly expressed miRNAs in seminoma, a primitive type of germ cell tumor. Formalin-fixed and paraffin-embedded (FFPE) surgical samples from 11 cases of normal testicular tissue resected for nonneoplastic causes and from 11 cases of seminoma were assessed for miRNA expression. Normal testicular tissue and seminoma were paired by race. We found 112 miRNAs to be differentially expressed between seminoma and normal testicular tissue; 52 miRNAs were overexpressed, and 60, downregulated in seminoma. We did not observe significant differences between black and white populations in our race-paired study. The upregulation of the expression of hsa-mir-21, hsa-mir-372, hsa-mir-373, has-mir-221, and hsa-mir-222 was validated by reverse transcription and real-time PCR. Hsa-mir-372 was upregulated around 1,270-fold (95 % confidence interval (CI) 525.2–3,064.8; p = 8.1e-5 by Mann–Whitney U test). Hsa-mir-373 was upregulated around 1,530-fold (95 % CI 620.5–3,785.6; p = 8.0e-5 by Mann–Whitney U test), consistent with previous reports, indicating that the miRNAs in FFPE are well preserved, and FFPE can be a valuable source for the miRNA study of seminoma. In addition, expression of hsa-mir-21 (12.2-fold, 0.0095), hsa-mir-221 (3.8-fold, 0.014) and hsa-mir-222 (3.8-fold, 0.019) was found elevated in seminoma compared to normal testicular tissue.
Literature
1.
McGlynn KA, Devesa SS, Sigurdson AJ et al (2003) Trends in the incidence of testicular germ cell tumors in the United States. Cancer 97(1):63–70PubMedCrossRef
2.
Iczkowski KA, Butler SL (2006) New immunohistochemical markers in testicular tumors. Anal Quant Cytol Histol 28(4):181–187PubMed
3.
4.
Voorhoeve PM, le Sage C, Schrier M et al (2006) A genetic screen implicates miRNA-372 and miRNA-373 as oncogenes in testicular germ cell tumors. Cell 124(6):1169–1181PubMedCrossRef
5.
Lee EJ, Gusev Y, Jiang J et al (2007) Expression profiling identifies microRNA signature in pancreatic cancer. Int J Cancer 120(5):1046–1054PubMedCrossRef
6.
Palmer RD, Murray MJ, Saini HK et al (2010) Malignant germ cell tumors display common microRNA profiles resulting in global changes in expression of messenger RNA targets. Cancer Res 70(7):2911–2923PubMedCrossRef
7.
8.
Heidenreich A, Weissbach L, Holtl W et al (2001) Organ sparing surgery for malignant germ cell tumor of the testis. J Urol 166(6):2161–2165PubMedCrossRef
9.
Di Vizio D, Cito L, Boccia A et al (2005) Loss of the tumor suppressor gene PTEN marks the transition from intratubular germ cell neoplasias (ITGCN) to invasive germ cell tumors. Oncogene 24(11):1882–1894PubMedCrossRef
10.
Tang F, Hajkova P, Barton SC et al (2006) MicroRNA expression profiling of single whole embryonic stem cells. Nucleic Acids Res 34(2):e9PubMedCrossRef
11.
Lehmann U (2010) MicroRNA-profiling in formalin-fixed paraffin-embedded specimens. Methods Mol Biol 667:113–125PubMedCrossRef
12.
Liu A, Xu X (2011) MicroRNA isolation from formalin-fixed, paraffin-embedded tissues. Methods Mol Biol 724:259–267PubMedCrossRef
13.
Luzna P, Gregar J, Uberall I et al (2011) Changes of microRNAs-192, 196a and 203 correlate with Barrett’s esophagus diagnosis and its progression compared to normal healthy individuals. Diagn Pathol 6(1):114PubMedCrossRef
14.
Niyazi M, Zehentmayr F, Niemoller OM et al (2011) MiRNA expression patterns predict survival in glioblastoma. Radiat Oncol 6:153PubMedCrossRef
15.
Snowdon J, Zhang X, Childs T et al (2011) The microRNA-200 family is upregulated in endometrial carcinoma. PLoS One 6(8):e22828PubMedCrossRef
16.
Tetzlaff MT, Liu A, Xu X et al (2007) Differential expression of miRNAs in papillary thyroid carcinoma compared to multinodular goiter using formalin fixed paraffin embedded tissues. Endocr Pathol 18(3):163–173PubMedCrossRef
17.
Gillis AJ, Stoop HJ, Hersmus R et al (2007) High-throughput microRNAome analysis in human germ cell tumours. J Pathol 213(3):319–328PubMedCrossRef
18.
Suh MR, Lee Y, Kim JY et al (2004) Human embryonic stem cells express a unique set of microRNAs. Dev Biol 270(2):488–498PubMedCrossRef
19.
Judson RL, Babiarz JE, Venere M et al (2009) Embryonic stem cell-specific microRNAs promote induced pluripotency. Nat Biotechnol 27(5):459–461PubMedCrossRef
20.
Wang Y, Baskerville S, Shenoy A et al (2008) Embryonic stem cell-specific microRNAs regulate the G1-S transition and promote rapid proliferation. Nat Genet 40(12):1478–1483PubMedCrossRef
21.
Voorhoeve PM, le Sage C, Schrier M et al (2007) A genetic screen implicates miRNA-372 and miRNA-373 as oncogenes in testicular germ cell tumors. Adv Exp Med Biol 604:17–46PubMedCrossRef
22.
Lowe SW, Ruley HE, Jacks T, Housman DE (1993) p53-dependent apoptosis modulates the cytotoxicity of anticancer agents. Cell 74(6):957–967PubMedCrossRef
23.
24.
Port M, Glaesener S, Ruf C et al (2011) Micro-RNA expression in cisplatin resistant germ cell tumor cell lines. Mol Cancer 10:52PubMedCrossRef
25.
Huang Q, Gumireddy K, Schrier M et al (2008) The microRNAs miR-373 and miR-520c promote tumour invasion and metastasis. Nat Cell Biol 10(2):202–210PubMedCrossRef
26.
Rentoft M, Fahlen J, Coates PJ et al (2011) miRNA analysis of formalin-fixed squamous cell carcinomas of the tongue is affected by age of the samples. Int J Oncol 38(1):61–69PubMed
27.
Siebolts U, Varnholt H, Drebber U et al (2009) Tissues from routine pathology archives are suitable for microRNA analyses by quantitative PCR. J Clin Pathol 62(1):84–88PubMedCrossRef
28.
Li S, Liang Z, Xu L, Zou F (2012) MicroRNA-21: a ubiquitously expressed pro-survival factor in cancer and other diseases. Mol Cell Biochem 360(1–2):147–158PubMedCrossRef
29.
Chan JA, Krichevsky AM, Kosik KS (2005) MicroRNA-21 is an antiapoptotic factor in human glioblastoma cells. Cancer Res 65(14):6029–6033PubMedCrossRef
30.
Frankel LB, Christoffersen NR, Jacobsen A et al (2008) Programmed cell death 4 (PDCD4) is an important functional target of the microRNA miR-21 in breast cancer cells. J Biol Chem 283(2):1026–1033PubMedCrossRef
31.
Asangani IA, Rasheed SA, Nikolova DA et al (2008) MicroRNA-21 (miR-21) post-transcriptionally downregulates tumor suppressor Pdcd4 and stimulates invasion, intravasation and metastasis in colorectal cancer. Oncogene 27(15):2128–2136PubMedCrossRef
32.
Nagao Y, Hisaoka M, Matsuyama A et al (2012) Association of microRNA-21 expression with its targets, PDCD4 and TIMP3, in pancreatic ductal adenocarcinoma. Mod Pathol 25(1):112–121PubMedCrossRef
33.
Niu Z, Goodyear SM, Rao S et al (2011) MicroRNA-21 regulates the self-renewal of mouse spermatogonial stem cells. Proc Natl Acad Sci U S A 108(31):12740–12745PubMedCrossRef
34.
Elmen J, Lindow M, Silahtaroglu A et al (2008) Antagonism of microRNA-122 in mice by systemically administered LNA-antimiR leads to up-regulation of a large set of predicted target mRNAs in the liver. Nucleic Acids Res 36(4):1153–1162PubMedCrossRef
35.
Krutzfeldt J, Rajewsky N, Braich R et al (2005) Silencing of microRNAs in vivo with ‘antagomirs’. Nature 438(7068):685–689PubMedCrossRef
36.
Felicetti F, Errico MC, Segnalini P, Mattia G, Care A (2008) MicroRNA-221 and −222 pathway controls melanoma progression. Expert Rev Anticancer Ther 8(11):1759–1765PubMedCrossRef
37.
le Sage C, Nagel R, Egan DA et al (2007) Regulation of the p27(Kip1) tumor suppressor by miR-221 and miR-222 promotes cancer cell proliferation. EMBO J 26(15):3699–3708PubMedCrossRef
38.
Liang Y (2008) An expression meta-analysis of predicted microRNA targets identifies a diagnostic signature for lung cancer. BMC Med Genomics 1:61PubMedCrossRef
39.
Miller TE, Ghoshal K, Ramaswamy B et al (2008) MicroRNA-221/222 confers tamoxifen resistance in breast cancer by targeting p27Kip1. J Biol Chem 283(44):29897–29903PubMedCrossRef
40.
Zhao JJ, Lin J, Yang H et al (2008) MicroRNA-221/222 negatively regulates estrogen receptor alpha and is associated with tamoxifen resistance in breast cancer. J Biol Chem 283(45):31079–31086PubMedCrossRef
41.
Garofalo M, Di Leva G, Romano G et al (2009) miR-221&222 regulate TRAIL resistance and enhance tumorigenicity through PTEN and TIMP3 downregulation. Cancer Cell 16(6):498–509PubMedCrossRef
42.
Zhang C, Kang C, Wang P et al (2011) MicroRNA-221 and −222 regulate radiation sensitivity by targeting the PTEN pathway. Int J Radiat Oncol Biol Phys 80(1):240–248PubMedCrossRef
Metadata
Title
MicroRNA expression profiles of seminoma from paraffin-embedded formalin-fixed tissue
Authors
Z. Bing
S. R. Master
J. W. Tobias
D. A. Baldwin
X. W. Xu
J. E. Tomaszewski
Publication date
01-12-2012
Publisher
Springer-Verlag
Published in
Virchows Archiv / Issue 6/2012
Print ISSN: 0945-6317
Electronic ISSN: 1432-2307
DOI
https://doi.org/10.1007/s00428-012-1325-9

Other articles of this Issue 6/2012

Virchows Archiv 6/2012 Go to the issue

Original Article

Absence of TCL1A expression is a useful diagnostic feature in splenic marginal zone lymphoma

Original Article

The presence of sinusoidal CD163+ macrophages in lymph nodes is associated with favorable nodal status in patients with breast cancer

Original Article

Histological characteristics of the human femoral head in patients with femoral neck fracture

Case Report

Bilateral Sertoli cell tumors of the testis—a likely new extracolonic manifestation of familial adenomatous polyposis

Original Article

Juvenile trabecular ossifying fibroma: an update

Original Article

BMI1 expression identifies subtypes of Merkel cell carcinoma