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Diagnostic Pathology
Published in: Diagnostic Pathology 1/2011

Open Access 01-12-2011 | Research

Changes of microRNAs-192, 196a and 203 correlate with Barrett's esophagus diagnosis and its progression compared to normal healthy individuals

Authors: Pavla Luzna, Jan Gregar, Ivo Uberall, Lenka Radova, Vlastimil Prochazka, Jiri Ehrmann Jr

Published in: Diagnostic Pathology | Issue 1/2011

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Abstract

Background

Barrett's esophagus (BE) is a disease with a rising prevalence in western countries probably due to the unhealthy lifestyle. In significant number of cases it develops to esophageal adenocarcinoma. Two decades ago, important gene regulators (microRNAs) were discovered and their attendance in the process of malignant transformation was demonstrated (e.g. miR-192, 196a, 203). Our aim was to select the patients with the increased risk of malignant transformation before the cancer develops.

Methods

71 patients with BE disease were selected, slides from FFPE blocks were prepared, the lesions were microdissected and a qPCR relative expression analysis for selected microRNAs (generally known to be connected with malignant transformation process) was carried out.

Results

We demonstrated unequivocal statistically significant upregulation of two microRNAs (miR-192, 196a) and downregulation of miR-203 and positive miR-196a correlation with progression from intestinal metaplasia to adenocarcinoma compared to normal individuals.

Conclusions

We hypothetize that there do exist changes of selected microRNAs which can undoubtedly distinguish the patients with BE from normal healthy individuals.
Appendix
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Literature
1.
Barrett N: Chronic peptic ulcer of the oesophagus and „oesophagitis“. Br J Surg. 1950, 30: 175-182.CrossRef
2.
Sternberg SS: Histology for Pathology. 1997, Philadelphia: Lippincott Williams & Wilkins
3.
Halvorsen JF, Semb BK: The "Barrett syndrome" (the columnar-lined lower oesophagus): an acquired condition secondary to reflux oesophagitis. A case report with discussion of pathogenesis. Acta Chir Scand. 1975, 141: 683-687.PubMed
4.
Gaddam S, Sharma P: Advances in endoscopic diagnosis and treatment of Barrett's esophagus. J Dig Dis. 2010, 11: 323-333. 10.1111/j.1751-2980.2010.00458.x.CrossRefPubMed
5.
Reid BJ, Li X, Galipeau PC, Vaughan TL: Barrett's oesophagus and oesophageal adenocarcinoma: time for a new synthesis. Nat Rev Cancer. 2010, 10: 87-101. 10.1038/nrc2773.PubMedCentralCrossRefPubMed
6.
Spechler SJ, Fitzgerald RC, Prasad GA, Wang KK: History, molecular mechanisms, and endoscopic treatment of Barrett's esophagus. Gastroenterology. 2010, 138: 854-869. 10.1053/j.gastro.2010.01.002.PubMedCentralCrossRefPubMed
7.
Paull A, Trier JS, Dalton MD, Camp RC, Loeb P, Goyal RK: The histologic spectrum of Barrett's esophagus. N Engl J Med. 1976, 295: 476-480. 10.1056/NEJM197608262950904.CrossRefPubMed
8.
Adler RH: The lower esophagus lined by columnar epithelium. Its association with hiatal hernia, ulcer, stricture, and tumor. J Thorac Cardiovasc Surg. 1963, 45: 13-34.PubMed
9.
Holmes RS, Vaughan TL: Epidemiology and pathogenesis of esophageal cancer. Semin Radiat Oncol. 2007, 17: 2-9. 10.1016/j.semradonc.2006.09.003.CrossRefPubMed
10.
11.
Griffiths-Jones S, Grocock RJ, van Dongen S, Bateman A, Enright AJ: miRBase: microRNA sequences, targets and gene nomenclature. Nucleic Acids Res. 2006, D140-144. 34 Database
12.
Griffiths-Jones S: The microRNA Registry. Nucleic Acids Res. 2004, D109-111. 32 Database
13.
Bagnyukova TV, Pogribny IP, Chekhun VF: MicroRNAs in normal and cancer cells: a new class of gene expression regulators. Exp Oncol. 2006, 28: 263-269.PubMed
14.
15.
Mirnezami AH, Pickard K, Zhang L, Primrose JN, Packham G: MicroRNAs: key players in carcinogenesis and novel therapeutic targets. Eur J Surg Oncol. 2009, 35: 339-347. 10.1016/j.ejso.2008.06.006.CrossRefPubMed
16.
Calin GA, Sevignani C, Dumitru CD, Hyslop T, Noch E, Yendamuri S, Shimizu M, Rattan S, Bullrich F, Negrini M, Croce CM: Human microRNA genes are frequently located at fragile sites and genomic regions involved in cancers. Proc Natl Acad Sci USA. 2004, 101: 2999-3004. 10.1073/pnas.0307323101.PubMedCentralCrossRefPubMed
17.
Blenkiron C, Miska EA: miRNAs in cancer: approaches, aetiology, diagnostics and therapy. Hum Mol Genet. 2007, 16 (Spec No 1): R106-113.CrossRefPubMed
18.
Kato M, Slack FJ: microRNAs: small molecules with big roles - C. elegans to human cancer. Biol Cell. 2008, 100: 71-81. 10.1042/BC20070078.CrossRefPubMed
19.
Feber A, Xi L, Luketich JD, Pennathur A, Landreneau RJ, Wu M, Swanson SJ, Godfrey TE, Litle VR: MicroRNA expression profiles of esophageal cancer. J Thorac Cardiovasc Surg. 2008, 135: 255-260. 10.1016/j.jtcvs.2007.08.055.PubMedCentralCrossRefPubMed
20.
Maru DM, Singh RR, Hannah C, Albarracin CT, Li YX, Abraham R, Romans AM, Yao H, Luthra MG, Anandasabapathy S, Swisher SG, Hofstetter WL, Rashid A, Luthra R: MicroRNA-196a is a potential marker of progression during Barrett's metaplasia-dysplasia-invasive adenocarcinoma sequence in esophagus. Am J Pathol. 2009, 174: 1940-1948. 10.2353/ajpath.2009.080718.PubMedCentralCrossRefPubMed
21.
Mathé EA, Nguyen GH, Bowman ED, Zhao Y, Budhu A, Schetter AJ, Braun R, Reimers M, Kumamoto K, Hughes D, Altorki NK, Casson AG, Liu CG, Wang XW, Yanaihara N, Hagiwara N, Dannenberg AJ, Miyashita M, Croce CM, Harris CC: MicroRNA expression in squamous cell carcinoma and adenocarcinoma of the esophagus: associations with survival. Clin Cancer Res. 2009, 15: 6192-6200. 10.1158/1078-0432.CCR-09-1467.PubMedCentralCrossRefPubMed
22.
Yang H, Gu J, Wang KK, Zhang W, Xing J, Chen Z, Ajani JA, Wu X: MicroRNA expression signatures in Barrett's esophagus and esophageal adenocarcinoma. Clin Cancer Res. 2009, 15: 5744-5752. 10.1158/1078-0432.CCR-09-0385.PubMedCentralCrossRefPubMed
23.
Specht K, Richter T, Müller U, Walch A, Werner M, Höfler H: Quantitative gene expression analysis in microdissected archival formalin-fixed and paraffin-embedded tumor tissue. Am J Pathol. 2001, 158: 419-429. 10.1016/S0002-9440(10)63985-5.PubMedCentralCrossRefPubMed
24.
Abrahamsen HN, Steiniche T, Nexo E, Hamilton-Dutoit SJ, Sorensen BS: Towards quantitative mRNA analysis in paraffin-embedded tissues using real-time reverse transcriptase-polymerase chain reaction: a methodological study on lymph nodes from melanoma patients. J Mol Diagn. 2003, 5: 34-41. 10.1016/S1525-1578(10)60449-7.PubMedCentralCrossRefPubMed
25.
Doleshal M, Magotra AA, Choudhury B, Cannon BD, Labourier E, Szafranska AE: Evaluation and validation of total RNA extraction methods for microRNA expression analyses in formalin-fixed, paraffin-embedded tissues. J Mol Diagn. 2008, 10: 203-211. 10.2353/jmoldx.2008.070153.PubMedCentralCrossRefPubMed
26.
Siebolts U, Varnholt H, Drebber U, Dienes HP, Wickenhauser C, Odenthal M: Tissues from routine pathology archives are suitable for microRNA analyses by quantitative PCR. J Clin Pathol. 2009, 62: 84-88. 10.1136/jcp.2008.058339.PubMedCentralCrossRefPubMed
27.
Nonn L, Vaishnav A, Gallagher L, Gann PH: mRNA and micro-RNA expression analysis in laser-capture microdissected prostate biopsies: valuable tool for risk assessment and prevention trials. Exp Mol Pathol. 2010, 88: 45-51. 10.1016/j.yexmp.2009.10.005.PubMedCentralCrossRefPubMed
28.
Noutsias M, Rohde M, Block A, Klippert K, Lettau O, Blunert K, Hummel M, Kühl U, Lehmkuhl H, Hetzer R, Rauch U, Poller W, Pauschinger M, Schultheiss HP, Volk HD, Kotsch K: Preamplification techniques for real-time RT-PCR analyses of endomyocardial biopsies. BMC Mol Biol. 2008, 9: 3-10.1186/1471-2199-9-3.PubMedCentralCrossRefPubMed
29.
30.
Vandesompele J, De Preter K, Pattyn F, Poppe B, Van Roy N, De Paepe A, Speleman F: Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genes. Genome Biol. 2002, 3: 34.1-34.12.CrossRef
31.
Mestdagh P, Van Vlierberghe P, De Weer A, Muth D, Westermann F, Speleman F, Vandesompele J: A novel and universal method for microRNA RT-qPCR data normalization. Genome Biol. 2009, 10: R64-10.1186/gb-2009-10-6-r64.PubMedCentralCrossRefPubMed
32.
R Development Core Team: R: A language and environment for statistical computing. 2011, R Foundation for Statistical Computing, Vienna, Austria, ISBN 3-900051-07-0, [http://​www.​R-project.​org/​]
33.
Pfaffl MW: A new mathematical model for relative quantification in real-time RT-PCR. Nucleic Acids Res. 2001, 29: 2002-2007.CrossRef
34.
O'Riordan JM, Abdel-latif MM, Ravi N, McNamara D, Byrne PJ, McDonald GS, Keeling PW, Kelleher D, Reynolds JV: Proinflammatory cytokine and nuclear factor kappa-B expression along the inflammation-metaplasia-dysplasia-adenocarcinoma sequence in the esophagus. Am J Gastroenterol. 2005, 100: 1257-1264. 10.1111/j.1572-0241.2005.41338.x.CrossRefPubMed
35.
Colleypriest BJ, Ward SG, Tosh D: How does inflammation cause Barrett's metaplasia?. Curr Opin Pharmacol. 2009, 9: 721-726. 10.1016/j.coph.2009.09.005.CrossRefPubMed
36.
Burgess JN, Payne WS, Andersen HA, Weiland LH, Carlson HC: Barrett esophagus: the columnar-epithelial-lined lower esophagus. Mayo Clin Proc. 1971, 46: 728-734.PubMed
37.
Szafranska AE, Doleshal M, Edmunds HS, Gordon S, Luttges J, Munding JB, Barth RJ, Gutmann EJ, Suriawinata AA, Marc Pipas J, Tannapfel A, Korc M, Hahn SA, Labourier E, Tsongalis GJ: Analysis of microRNAs in pancreatic fine-needle aspirates can classify benign and malignant tissues. Clin Chem. 2008, 54: 1716-1724. 10.1373/clinchem.2008.109603.PubMedCentralCrossRefPubMed
38.
Bloomston M, Frankel WL, Petrocca F, Volinia S, Alder H, Hagan JP, Liu CG, Bhatt D, Taccioli C, Croce CM: MicroRNA expression patterns to differentiate pancreatic adenocarcinoma from normal pancreas and chronic pancreatitis. JAMA. 2007, 297: 1901-1908. 10.1001/jama.297.17.1901.CrossRefPubMed
39.
Kumarswamy R, Volkmann I, Thum T: Regulation and function of miRNA-21 in health and disease. RNA Biol. 2011, 8: 1-8. 10.4161/rna.8.1.15302.CrossRef
40.
Saini S, Majid S, Yamamura S, Tabatabai ZL, Suh SO, Shahryari V, Chen Y, Deng G, Tanaka Y, Dahiya R: Regulatory role of miR-203 in prostate cancer progression and metastasis. Clin Cancer Res. 2011, 17: 5287-5298. 10.1158/1078-0432.CCR-10-2619.CrossRefPubMed
Metadata
Title
Changes of microRNAs-192, 196a and 203 correlate with Barrett's esophagus diagnosis and its progression compared to normal healthy individuals
Authors
Pavla Luzna
Jan Gregar
Ivo Uberall
Lenka Radova
Vlastimil Prochazka
Jiri Ehrmann Jr
Publication date
01-12-2011
Publisher
BioMed Central
Published in
Diagnostic Pathology / Issue 1/2011
Electronic ISSN: 1746-1596
DOI
https://doi.org/10.1186/1746-1596-6-114

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