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BMC Oral Health
Published in: BMC Oral Health 1/2023

Open Access 01-12-2023 | Metformin | Research

Effects of metformin on human gingival fibroblasts: an in vitro study

Authors: Nouf Alshibani, Reem AlKattan, Eman Allam, Fahad A. Alshehri, Manal Matouq Shalabi, Nada Almuhanna, Houriah Almarshad, Anfal Aljamili

Published in: BMC Oral Health | Issue 1/2023

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Abstract

Objective

To investigate the effects of metformin (MF) treatment on the matrix metalloproteinases (MMPs) and proinflammatory cytokines production from lipopolysaccharide (LPS) - stimulated human gingival fibroblasts (HGFs).

Methods

HGFs were obtained from subcultures of biopsies from clinically healthy gingival tissues of patients undergoing oral surgeries. Cell cytotoxicity assay was used to determine the effect of different concentrations of MF on viability of HGFs. HGFs were then incubated and treated with different concentrations of MF and Porphyromonas gingivais (Pg) LPS. MMP-1, MMP-2, MMP-8, MMP-9, IL-1β, and IL-8 expression analysis was performed using xMAP technology (Luminex 200, Luminex, Austin, TX, USA). Student’s t-test for a single sample was used to compare the mean values of the study groups with the control value. A p-value of <0.05 and 95% confidence intervals were used to report the statistical significance and precision of mean values.

Results

Concentrations of 0.5, 1- and 2-mM MF had a minimal non-significant cytotoxic effect on the HGFs and caused statistically significant reduction of MMP-1, MMP-2, MMP-8 and IL-8 expressed by the LPS-stimulated HGFs.

Conclusion

The results of the present study confirm that MF suppresses MMP-1, MMP-2, MMP-8 and IL-8 in LPS-stimulated HGFs suggesting an anti-inflammatory effect of MF and potential adjunct therapeutic role in the treatment of periodontal diseases.
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Metadata
Title
Effects of metformin on human gingival fibroblasts: an in vitro study
Authors
Nouf Alshibani
Reem AlKattan
Eman Allam
Fahad A. Alshehri
Manal Matouq Shalabi
Nada Almuhanna
Houriah Almarshad
Anfal Aljamili
Publication date
01-12-2023
Publisher
BioMed Central
Published in
BMC Oral Health / Issue 1/2023
Electronic ISSN: 1472-6831
DOI
https://doi.org/10.1186/s12903-023-02978-0

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