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BMC Oral Health
Published in: BMC Oral Health 1/2023

Open Access 01-12-2023 | Research

The effect of carbodiimide on push-out bond strength of fiber posts and endogenous enzymatic activity

Authors: Uros Josic, Claudia Mazzitelli, Tatjana Maravic, Allegra Comba, Milena Cadenaro, Ivana Radovic, Maicon Sebold, Gianluca Turco, Lorenzo Breschi, Annalisa Mazzoni

Published in: BMC Oral Health | Issue 1/2023

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Abstract

Background

To investigate the effect of 0.3 M 1-ethyl-3(3-dimethylaminopropyl) carbodiimide (EDC) aqueous solution pretreatment on push-out bond strength (PBS) and matrix-metalloproteinases (MMPs) activity within radicular dentin when different post cementation strategies were employed.

Methods

One hundred and twenty monoradicular human teeth were endodontically treated and randomly divided into six groups, depending on the cementation strategy and root dentin pretreatment (n = 20): EAR: cementation with an etch-and-rinse adhesive (LuxaBond Total Etch, DMG) and resin cement (LuxaCore Z Dual, DMG); EAR/EDC: 1 min EDC pretreatment after etching + EAR; SE: cementation with a self-etch primer (Multilink Primer, Ivoclar Vivadent) and corresponding cement (Multilink Automix, Ivoclar Vivadent); SE/EDC: self-etch primer + EDC pretreatment + SE; SA: cementation with a universal self-adhesive cement (RelyX Universal, 3 M); SA/EDC: EDC pretreatment + SA. Slices were submitted to PBS test and interfacial nanoleakage evaluation 24 h after cementation or after thermocycling (40.000 cycles, 5–55 °C). To investigate the effect of EDC on MMPs activity, 4 additional first maxillary premolars per group were processed for in situ zymography analysis. Multivariate ANOVA and post hoc Tukey tests were used to analyze PBS values. The data from in situ zymography were analyzed with Kruskal-Wallis test and Dunn’s pairwise multiple comparison procedures (α = 0.05).

Results

The variables “EDC pretreatment”, “root region” and “thermocycling” significantly influenced PBS (p < 0.05), while the variable “cementation strategy” had no influence (p > 0.05). Thermocycling significantly reduced PBS in SE and SA groups (p < 0.05). EDC was effective in preserving PBS after artificial aging. EDC pretreatment significantly reduced enzymatic activity at baseline in EAR and SE groups, and in SA group after thermocycling (p < 0.05).

Conclusions

The use of EDC prevents the reduction of bond-strength values after artificial aging and silences endogenous enzymatic activity within radicular dentin when different cementation strategies were employed.
Literature
1.
Radovic I, Mazzitelli C, Chieffi N, Ferrari M. Evaluation of the adhesion of fiber posts cemented using different adhesive approaches. Eur J Oral Sci. 2008;116(6):557–63.CrossRefPubMed
2.
Maravić T, Mazzitelli C, Mancuso E, Del Bianco F, Josić U, Cadenaro M et al. Resin composite cements: current status and a novel classification proposal. J Esthetic Restor Dentistry. 2023.
3.
Mazzitelli C, Maravic T, Mancuso E, Josic U, Generali L, Comba A, et al. Influence of the activation mode on long-term bond strength and endogenous enzymatic activity of dual-cure resin cements. Clin Oral Invest. 2022;26(2):1683–94.CrossRef
4.
Maravic T, Mazzoni A, Comba A, Scotti N, Checchi V, Breschi L. How stable is dentin as a substrate for bonding? Curr Oral Health Rep. 2017;4(3):248–57.CrossRef
5.
Breschi L, Maravic T, Cunha SR, Comba A, Cadenaro M, Tjaderhane L, et al. Dentin bonding systems: from dentin collagen structure to bond preservation and clinical applications. Dent Mater. 2018;34(1):78–96.CrossRefPubMed
6.
Ozcan M, Volpato CAM. Current perspectives on dental adhesion: (3) adhesion to intraradicular dentin: concepts and applications. Jpn Dent Sci Rev. 2020;56(1):216–23.CrossRefPubMedPubMedCentral
7.
Ferracane JL, Stansbury JW, Burke FJ. Self-adhesive resin cements - chemistry, properties and clinical considerations. J Oral Rehabil. 2011;38(4):295–314.CrossRefPubMed
8.
Scholz KJ, Bittner A, Cieplik F, Hiller KA, Schmalz G, Buchalla W et al. Micromorphology of the Adhesive Interface of Self-Adhesive Resin cements to enamel and dentin. Mater (Basel). 2021;14(3).
9.
Ubaldini ALM, Benetti AR, Sato F, Pascotto RC, Medina Neto A, Baesso ML, et al. Challenges in luting fibre posts: adhesion to the post and to the dentine. Dent Mater. 2018;34(7):1054–62.CrossRefPubMed
10.
Ferrari M, Mason PN, Goracci C, Pashley DH, Tay FR. Collagen degradation in endodontically treated teeth after clinical function. J Dent Res. 2004;83(5):414–9.CrossRefPubMed
11.
Breschi L, Mazzoni A, Ruggeri A, Cadenaro M, Di Lenarda R, De Stefano Dorigo E. Dental adhesion review: aging and stability of the bonded interface. Dent Mater. 2008;24(1):90–101.CrossRefPubMed
12.
Santos J, Carrilho M, Tervahartiala T, Sorsa T, Breschi L, Mazzoni A, et al. Determination of matrix metalloproteinases in human radicular dentin. J Endod. 2009;35(5):686–9.CrossRefPubMed
13.
Yu H, Liu J, Liao Z, Yu F, Qiu B, Zhou M, et al. Location of MMPs in human radicular dentin and the effects of MMPs inhibitor on the bonding stability of fiber posts to radicular dentin. J Mech Behav Biomed Mater. 2022;129:105144.CrossRefPubMed
14.
Comba A, Maravić T, Villalta V, Tozzola S, Mazzitelli C, Checchi V, et al. Effect of an ethanol cross-linker on universal adhesive. Dent Mater. 2020;36(12):1645–54.CrossRefPubMed
15.
Frassetto A, Breschi L, Turco G, Marchesi G, Di Lenarda R, Tay FR, et al. Mechanisms of degradation of the hybrid layer in adhesive dentistry and therapeutic agents to improve bond durability–A literature review. Dent Mater. 2016;32(2):e41–53.CrossRefPubMed
16.
Cadenaro M, Josic U, Maravić T, Mazzitelli C, Marchesi G, Mancuso E, et al. Progress in dental adhesive materials. J Dent Res. 2023;102(3):254–62.CrossRefPubMed
17.
Mazzoni A, Angeloni V, Apolonio FM, Scotti N, Tjaderhane L, Tezvergil-Mutluay A, et al. Effect of carbodiimide (EDC) on the bond stability of etch-and-rinse adhesive systems. Dent Mater. 2013;29(10):1040–7.CrossRefPubMed
18.
Mazzoni A, Angeloni V, Comba A, Maravic T, Cadenaro M, Tezvergil-Mutluay A, et al. Cross-linking effect on dentin bond strength and MMPs activity. Dent Mater. 2018;34(2):288–95.CrossRefPubMed
19.
Mazzoni A, Angeloni V, Sartori N, Duarte S Jr, Maravic T, Tjaderhane L, et al. Substantivity of Carbodiimide inhibition on dentinal enzyme activity over Time. J Dent Res. 2017;96(8):902–8.CrossRefPubMed
20.
Mazzoni A, Apolonio FM, Saboia VP, Santi S, Angeloni V, Checchi V, et al. Carbodiimide inactivation of MMPs and effect on dentin bonding. J Dent Res. 2014;93(3):263–8.CrossRefPubMedPubMedCentral
21.
Maravic T, Mancuso E, Comba A, Checchi V, Generali L, Mazzitelli C et al. Dentin cross-linking effect of Carbodiimide after 5 years. J Dent Res. 2021:220345211014799.
22.
Alonso JRL, Basso FG, Scheffel DLS, de-Souza-Costa CA, Hebling J. Effect of crosslinkers on bond strength stability of fiber posts to root canal dentin and in situ proteolytic activity. J Prosthet Dent. 2018;119(3):494. e1- e9.CrossRef
23.
Comba A, Scotti N, Mazzoni A, Maravic T, Ribeiro Cunha S, Michelotto Tempesta R, et al. Carbodiimide inactivation of matrix metalloproteinases in radicular dentine. J Dent. 2019;82:56–62.CrossRefPubMed
24.
Lopes FC, Roperto R, Akkus A, de Queiroz AM, Francisco de Oliveira H, Sousa-Neto MD. Effect of carbodiimide and chlorhexidine on the bond strength longevity of resin cement to root dentine after radiation therapy. Int Endod J. 2020;53(4):539–52.CrossRefPubMed
25.
Shafiei F, Yousefipour B, Mohammadi-Bassir M. Effect of Carbodiimide on Bonding durability of Adhesive-cemented Fiber posts in Root Canals. Oper Dent. 2016;41(4):432–40.CrossRefPubMed
26.
Bueno MR, Estrela C, Azevedo BC, Cintra Junqueira JL. Root Canal shape of Human Permanent Teeth determined by New Cone-Beam Computed Tomographic Software. J Endod. 2020;46(11):1662–74.CrossRefPubMed
27.
Armstrong S, Breschi L, Ozcan M, Pfefferkorn F, Ferrari M, Van Meerbeek B. Academy of Dental Materials guidance on in vitro testing of dental composite bonding effectiveness to dentin/enamel using micro-tensile bond strength (muTBS) approach. Dent Mater. 2017;33(2):133–43.CrossRefPubMed
28.
Mazzoni A, Marchesi G, Cadenaro M, Mazzotti G, Di Lenarda R, Ferrari M, et al. Push-out stress for fibre posts luted using different adhesive strategies. Eur J Oral Sci. 2009;117(4):447–53.CrossRefPubMed
29.
Delgado AH, Sauro S, Lima AF, Loguercio AD, Della Bona A, Mazzoni A, et al. RoBDEMAT: a risk of bias tool and guideline to support reporting of pre-clinical dental materials research and assessment of systematic reviews. J Dent. 2022;127:104350.CrossRefPubMed
30.
Saboia VP, Nato F, Mazzoni A, Orsini G, Putignano A, Giannini M, et al. Adhesion of a two-step etch-and-rinse adhesive on collagen-depleted dentin. J Adhes Dent. 2008;10(6):419–22.PubMed
31.
Josic U, Mazzitelli C, Maravic T, Comba A, Mayer-Santos E, Florenzano F, et al. Evaluation of Fiber Post Adhesion to Root dentin achieved with Different Composite cements: 1-year in Vitro results. J Adhes Dent. 2022;24(1):95–104.PubMed
32.
Josic U, Maravic T, Bossù M, Cadenaro M, Comba A, Ierardo G, et al. Morphological characterization of deciduous enamel and dentin in patients affected by Osteogenesis Imperfecta. Appl Sci. 2020;10(21):7835.CrossRef
33.
Sorrentino R, Di Mauro MI, Ferrari M, Leone R, Zarone F. Complications of endodontically treated teeth restored with fiber posts and single crowns or fixed dental prostheses-a systematic review. Clin Oral Investig. 2016;20(7):1449–57.CrossRefPubMed
34.
Mazzoni A, Scaffa P, Carrilho M, Tjaderhane L, Di Lenarda R, Polimeni A, et al. Effects of etch-and-rinse and self-etch adhesives on dentin MMP-2 and MMP-9. J Dent Res. 2013;92(1):82–6.CrossRefPubMedPubMedCentral
35.
Josic U, Maravic T, Mazzitelli C, Del Bianco F, Mazzoni A, Breschi L. The effect of chlorhexidine primer application on the clinical performance of composite restorations: a literature review. J Esthet Restor Dent. 2020.
36.
Amaral SFD, Scaffa PMC, Rodrigues RDS, Nesadal D, Marques MM, Nogueira FN, et al. Dynamic influence of pH on metalloproteinase activity in human coronal and radicular dentin. Caries Res. 2018;52(1–2):113–8.CrossRefPubMed
37.
Yaman BC, Ozer F, Takeichi T, Karabucak B, Koray F, Blatz MB. Effect of thermomechanical aging on bond strength and interface morphology of glass fiber and zirconia posts bonded with a self-etch adhesive and a self-adhesive resin cement to natural teeth. J Prosthet Dent. 2014;112(3):455–64.CrossRefPubMed
38.
Dimitrouli M, Geurtsen W, Luhrs AK. Comparison of the push-out strength of two fiber post systems dependent on different types of resin cements. Clin Oral Investig. 2012;16(3):899–908.CrossRefPubMed
39.
De Munck J, Van Landuyt K, Peumans M, Poitevin A, Lambrechts P, Braem M, et al. A critical review of the durability of adhesion to tooth tissue: methods and results. J Dent Res. 2005;84(2):118–32.CrossRefPubMed
40.
Breschi L, Maravic T, Comba A, Cunha SR, Loguercio AD, Reis A, et al. Chlorhexidine preserves the hybrid layer in vitro after 10-years aging. Dent Mater. 2020;36(5):672–80.CrossRefPubMed
41.
Tezvergil-Mutluay A, Agee KA, Hoshika T, Carrilho M, Breschi L, Tjaderhane L, et al. The requirement of zinc and calcium ions for functional MMP activity in demineralized dentin matrices. Dent Mater. 2010;26(11):1059–67.CrossRefPubMedPubMedCentral
42.
Sarkis-Onofre R, Pereira-Cenci T, Opdam NJ, Demarco FF. Preference for using posts to restore endodontically treated teeth: findings from a survey with dentists. Brazilian oral research. 2014;29:1–6.CrossRefPubMed
43.
Skupien JA, Sarkis-Onofre R, Cenci MS, RRd MORAES, Pereira-Cenci T. A systematic review of factors associated with the retention of glass fiber posts. Brazilian oral research. 2015;29:1–8.
44.
Sarkis-Onofre R, Skupien JA, Cenci MS, Moraes RR, Pereira-Cenci T. The role of resin cement on bond strength of glass-fiber posts luted into root canals: a systematic review and meta-analysis of in vitro studies. Oper Dent. 2014;39(1):E31–44.CrossRefPubMed
Metadata
Title
The effect of carbodiimide on push-out bond strength of fiber posts and endogenous enzymatic activity
Authors
Uros Josic
Claudia Mazzitelli
Tatjana Maravic
Allegra Comba
Milena Cadenaro
Ivana Radovic
Maicon Sebold
Gianluca Turco
Lorenzo Breschi
Annalisa Mazzoni
Publication date
01-12-2023
Publisher
BioMed Central
Published in
BMC Oral Health / Issue 1/2023
Electronic ISSN: 1472-6831
DOI
https://doi.org/10.1186/s12903-023-03067-y

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