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Journal of Cancer Research and Clinical Oncology

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01-12-2020 | Metastasis | Original Article – Cancer Research

UV-type specific alteration of miRNA expression and its association with tumor progression and metastasis in SCC cell lines

Authors: I-Peng Chen, Marc Bender, Ivelina Spassova, Stefan Henning, Linda Kubat, Kaiji Fan, Sarah Degenhardt, Mouna Mhamdi-Ghodbani, Ashwin Sriram, Beate Volkmer, Petra Boukamp, Jürgen C. Becker, Rüdiger Greinert

Published in: Journal of Cancer Research and Clinical Oncology | Issue 12/2020

Abstract

Purpose

UV exposure is the main risk factor for development of cutaneous squamous cell carcinoma (cSCC). While early detection greatly improves cSCC prognosis, locally advanced or metastatic cSCC has a severely impaired prognosis. Notably, the mechanisms of progression to metastatic cSCC are not well understood. We hypothesized that UV exposure of already transformed epithelial cSCC cells further induces changes which might be involved in the progression to metastatic cSCCs and that UV-inducible microRNAs (miRNAs) might play an important role.

Methods

Thus, we analyzed the impact of UV radiation of different quality (UVA, UVB, UVA + UVB) on the miRNA expression pattern in established cell lines generated from primary and metastatic cSCCs (Met-1, Met-4) using the NanoString nCounter platform.

Results

This analysis revealed that the expression pattern of miRNAs depends on both the cell line used per se and on the quality of UV radiation. Comparison of UV-induced miRNAs in cSCC cell lines established from a primary tumor (Met-1) and the respective (un-irradiated) metastasis (Met-4) suggest that miR-7-5p, miR-29a-3p and miR-183-5p are involved in a UV-driven pathway of progression to metastasis. This notion is supported by the fact that these three miRNAs build up a network of 81 potential target genes involved e.g. in UVA/UVB-induced MAPK signaling and regulation of the epithelial–mesenchymal transition. As an example, PTEN, a target of UV-upregulated miRNAs (miR-29a-3p, miR-183-5p), could be shown to be down-regulated in response to UV radiation. We further identified CNOT8, the transcription complex subunit 8 of the CCR4-NOT complex, a deadenylase removing the poly(A) tail from miRNA-destabilized mRNAs, in the center of this network, targeted by all three miRNAs.

Conclusion

In summary, our results demonstrate that UV radiation induces an miRNA expression pattern in primary SCC cell line partly resembling those of metastatic cell line, thus suggesting that UV radiation impacts SCC progression beyond initiation.

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Title: UV-type specific alteration of miRNA expression and its association with tumor progression and metastasis in SCC cell lines
Authors: I-Peng Chen
Marc Bender
Ivelina Spassova
Stefan Henning
Linda Kubat
Kaiji Fan
Sarah Degenhardt
Mouna Mhamdi-Ghodbani
Ashwin Sriram
Beate Volkmer
Petra Boukamp
Jürgen C. Becker
Rüdiger Greinert
Publication date: 01-12-2020
Publisher: Springer Berlin Heidelberg
Keywords: Metastasis
Cutaneous Squamous Cell Carcinoma
Cutaneous Squamous Cell Carcinoma
Skin Cancer
Published in: Journal of Cancer Research and Clinical Oncology / Issue 12/2020
Print ISSN: 0171-5216
Electronic ISSN: 1432-1335
DOI: https://doi.org/10.1007/s00432-020-03358-9

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Prof. Kevin Dolgin

Prof. Florian Limbourg

Prof. Anoop Chauhan

Developed by: Springer Medicine

Obesity Clinical Trial Summary

At a glance: The STEP trials

A round-up of the STEP phase 3 clinical trials evaluating semaglutide for weight loss in people with overweight or obesity.

Developed by: Springer Medicine

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

Purpose

Methods

Results

Conclusion

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Other articles of this Issue 12/2020

Correction to: Hyperprogressive disease and its clinical impact in patients with recurrent and/or metastatic head and neck squamous cell carcinoma treated with immune-checkpoint inhibitors: Korean cancer study group HN 18–12

Patient expectations are better for immunotherapy than traditional chemotherapy for cancer

Clinical characteristics and comparison of the outcome in young versus older patients with upper gastrointestinal carcinoma

Sentinel lymph node intraoperative analysis in endometrial cancer

Implications of flavonoids as potential modulators of cancer neovascularity

Senescent hepatic stellate cells caused by deoxycholic acid modulates malignant behavior of hepatocellular carcinoma

Keynote webinar | Spotlight on medication adherence

Live: Thursday 27th June 2024, 18:00-19:30 (CEST)

At a glance: The STEP trials