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BMC Cancer

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Open Access 01-12-2024 | Lung Cancer | Research

Identification of key genes in chronic intermittent hypoxia-induced lung cancer progression based on transcriptome sequencing

Authors: Li-Da Chen, Li Lin, Ji-Zhi Chen, Yang Song, Wei-Liang Zhang, Huang-Yu Li, Jia-Min Luo, Xiao-Bin Zhang

Published in: BMC Cancer | Issue 1/2024

Abstract

Background

Obstructive sleep apnea (OSA) is associated with increased risk of lung cancer mortality. Nevertheless, little is known about the underlying molecular mechanisms. This research aimed to investigate differentially expressed genes (DEGs) and explore their function in Lewis lung carcinoma (LLC)-bearing mice exposed to chronic intermittent hypoxia (CIH) by transcriptome sequencing.

Methods

Lung cancer tissues in LLC-bearing mice exposed to CIH or normoxia were subjected for transcriptome sequencing to examine DEGs. Gene Ontology and Kyoto Encyclopedia of Genes and Genomes pathway analyses were employed to explore the function of DEGs. To evaluate the prognostic value of DEGs, the Kaplan–Meier survival analysis in combination with Cox proportional hazard model were applied based on The Cancer Genome Atlas.

Results

A total of 388 genes with 207 up-regulated and 181 down-regulated genes were differentially expressed between the CIH and normoxia control groups. Bioinformatics analysis revealed that the DEGs were related to various signaling pathways such as chemokine signaling pathway, IL-17 signaling pathway, TGF-β signaling pathway, transcriptional misregulation in cancer, natural killer cell mediated cytotoxicity, PPAR signaling pathway. In addition, the DEGs including APOL1, ETFB, KLK8, PPP1R3G, PRL, SPTA1, PLA2G3, PCP4L1, NINJ2, MIR186, and KLRG1 were proven to be significantly correlated with poorer overall survival in lung adenocarcinoma.

Conclusions

CIH caused a significant change of gene expression profiling in LLC-bearing mice. The DEGs were found to be involved in various physiological and pathological processes and correlated with poorer prognosis in lung cancer.

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Title: Identification of key genes in chronic intermittent hypoxia-induced lung cancer progression based on transcriptome sequencing
Authors: Li-Da Chen
Li Lin
Ji-Zhi Chen
Yang Song
Wei-Liang Zhang
Huang-Yu Li
Jia-Min Luo
Xiao-Bin Zhang
Publication date: 01-12-2024
Publisher: BioMed Central
Keywords: Lung Cancer
Lung Cancer
Lung Cancer
Sleep Apnea
Published in: BMC Cancer / Issue 1/2024
Electronic ISSN: 1471-2407
DOI: https://doi.org/10.1186/s12885-023-11785-3

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