Top

Journal of Orthopaedic Surgery and Research

Published in:

Open Access 01-12-2024 | Lumbar Disc Herniation | Research article

Mechanism exploration of Osteoking in the treatment of lumbar disc herniation based on network pharmacology and molecular docking

Authors: Xinlei Luo, Jingjing Liu, Xiaoxi Wang, Qiaojun Chen, Yanfa Lei, Zewei He, Xiaowei Wang, Yan Ye, Qiang Na, Changtao Lao, Zhengchang Yang, Jun Jiang

Published in: Journal of Orthopaedic Surgery and Research | Issue 1/2024

Abstract

Objective

Lumbar disc herniation (LDH) is a common spinal surgical disease. Low back and leg pain caused by LDH is the main factor leading to functional disability, which has caused a serious burden to patients and society. Osteoking can delay the progression of osteoporosis and osteoarthritis, and even has a significant effect on the prevention of deep vein thrombosis after fracture surgery. In recent years, it has been gradually used in the treatment of LDH and has received significant results. However, the underlying mechanism remains unclear. The aim of this study was to predict the mechanism of Osteoking in the treatment of LDH through network pharmacology and verify it by molecular docking method.

Methods

The TCMSP database was used to collect the relevant active components and targets of Osteoking, while the GeneCards, OMIM and DisGeNET databases were utilized to collect the relevant disease targets of LDH. The Venny 2.1.0 software was employed to obtain the intersecting gene targets of Osteoking and LDH. PPI network construction and core target selection were performed using Cytoscape 3.9.0 software. The Metascape database was used for GO and KEGG enrichment analysis of the relevant targets. Finally, molecular docking was conducted using AutoDock software.

Results

The study identified 116 potential targets and 26 core targets for the treatment of LDH with Osteoking. Pathways in cancer, Alzheimer's disease, microRNAs in cancer and the IL-17 signalling pathway were among the main involved signalling pathways. Molecular docking results demonstrated that the key targets AKT1, IL-6, ALB, TNF and IL-1β exhibited relatively stable binding activities with the main active components of Osteoking.

Conclusions

Osteoking can alleviate the symptoms of lumbar disc herniation through the modulation of multiple targets and signalling pathways.

Wei X, Gengwu L, Chao C, et al. Correlations between the sagittal plane parameters of the spine and pelvis and lumbar disc degeneration. J Orthop Surg Res. 2018;13(1):137.CrossRefPubMedPubMedCentral

Kos N, Gradisnik L, Velnar T. A brief review of the degenerative intervertebral disc disease. Med Arch. 2019;73(6):421–4.CrossRefPubMedPubMedCentral

Hartvigsen J, Hancock MJ, Kongsted A, et al. What low back pain is and why we need to pay attention. Lancet. 2018;391(10137):2356–67.CrossRefPubMed

Le VT, Nguyen Dao LT, Nguyen AM. Transforaminal injection of autologous platelet-rich plasma for lumbar disc herniation: a single-center prospective study in Vietnam. Asian J Surg. 2023;46(1):438–43.CrossRefPubMed

Hartvigsen J, Hancock MJ, Kongsted A, et al. Lancet Low Back Pain Series Working Group: What low back pain is and why we need to pay attention. Lancet. 2018;391(10137):2356–67.CrossRefPubMed

Horner HA, Urban JP. Volvo Award Winner in Basic Science Studies: Effect of nutrient supply on the viability of cells from the nucleus pulposus of the intervertebral disc. Spine. 2001;26(23):2543–9.CrossRefPubMed

Sive JI, Baird P, Jeziorsk M, et al. Expression of chondrocyte markers by cells of normal and degenerate intervertebral discs. Mol Pathol. 2002;55(2):91–7.CrossRefPubMedPubMedCentral

Le Maitre CL, Freemont AJ, Hoyland JA. Localization of degradative enzymes and their inhibitors in the degenerate human intervertebral disc. J Pathol. 2004;204(1):47–54.CrossRefPubMed

Wang L, He T, Liu J, Tai J, Wang B, Zhang L, Quan Z. Revealing the immune infiltration landscape and identifying diagnostic biomarkers for lumbar disc herniation. Front Immunol. 2021;27(12): 666355.CrossRef

10.

Dai L, Wu H, Yu S, et al. Effects of OsteoKing on osteoporotic rabbits. Mol Med Rep. 2015;12(1):1066–74.CrossRefPubMedPubMedCentral

11.

Sun Y, Wang X, Chen R, et al. Osteoking downregulates Mgp in an osteoporotic fracture rat model. J Tradit Chin Med. 2020;40(3):422–31.PubMed

12.

Ling H, Zeng Q, Ge Q, et al. Osteoking decelerates cartilage degeneration in dmm-induced osteoarthritic mice model through TGF-β/smad-dependent manner. Front Pharmacol. 2021;15(12): 678810.CrossRef

13.

Zhao HB, Hu M, Zheng HY, et al. Clinical study on effect of Osteoking in preventing postoperational deep venous thrombosis in patients with intertrochanteric fracture. Chin J Integr Med. 2005;11(4):297–9.CrossRefPubMed

14.

Yu C, Dai L, Ma Z, et al. Effect of Osteoking on the osteogenic and adipogenic differentiation potential of rat bone marrow mesenchymal stem cells in vitro. BMC Complement Altern Med. 2019;19(1):36.CrossRefPubMedPubMedCentral

15.

Hsin KY, Ghosh S, Kitano H. Combining machine learning systems and multiple docking simulation packages to improve docking prediction reliability for network pharmacology. PLoS ONE. 2013;8(12): e83922.CrossRefPubMedPubMedCentral

16.

Chen ZH, Jin SH, Wang MY, et al. Enhanced NLRP3, caspase-1, and IL- 1β levels in degenerate human intervertebral disc and their association with the grades of disc degeneration. Anat Rec (Hoboken). 2015;298(4):720–6.CrossRefPubMed

17.

Wang Y, Che M, Xin J, et al. The role of IL-1β and TNF-α in intervertebral disc degeneration. Biomed Pharmacother. 2020;131: 110660.CrossRefPubMed

18.

Kang R, Li H, Rickers K, et al. Intervertebral disc degenerative changes after intradiscal injection of TNF-α in a porcine model. Eur Spine J. 2015;24(9):2010–6.CrossRefPubMed

19.

Moon HJ, Kim JH, Lee HS, et al. Annulus fibrosus cells interact with neuron-like cells to modulate production of growth factors and cytokines in symptomatic disc degeneration. Spine. 2012;37(1):2–9.CrossRefPubMed

20.

Chistiakov DA, Bobryshev YV, Orekhov AN. Changes in transcriptome of macrophages in atherosclerosis. J Cell Mol Med. 2015;19(6):1163–73.CrossRefPubMedPubMedCentral

21.

Jia A, Wang Y, Wang Y, et al. The kinase AKT1 potentiates the suppressive functions of myeloid-derived suppressor cells in inflammation and cancer. Cell Mol Immunol. 2021;18(4):1074–6.CrossRefPubMedPubMedCentral

22.

Hu B, Xu C, Tian Y, et al. Inflammatory microRNA-194 and -515 attenuate the biosynthesis of chondroitin sulfate during human intervertebral disc degeneration. Oncotarget. 2017;8(30):49303–17.CrossRefPubMedPubMedCentral

23.

Kong L, Sun M, Jiang Z, et al. MicroRNA-194 inhibits lipopolysaccharide-induced inflammatory response in nucleus pulposus cells of the intervertebral disc by targeting TNF receptor-associated factor 6 (TRAF6). Med Sci Monit. 2018;10(24):3056–67.CrossRef

24.

Jiang H, Deng Y, Wang T, et al. Interleukin-23 may contribute to the pathogenesis of lumbar disc herniation through the IL-23/IL-17 pathway. J Orthop Surg Res. 2016;16(11):12.CrossRef

25.

Gabr MA, Jing L, Helbling AR, et al. Interleukin-17 synergizes with IFNγ or TNFα to promote inflammatory mediator release and intercellular adhesion molecule-1 (ICAM-1) expression in human intervertebral disc cells. J Orthop Res. 2011;29(1):1–7.CrossRefPubMedPubMedCentral

Title: Mechanism exploration of Osteoking in the treatment of lumbar disc herniation based on network pharmacology and molecular docking
Authors: Xinlei Luo
Jingjing Liu
Xiaoxi Wang
Qiaojun Chen
Yanfa Lei
Zewei He
Xiaowei Wang
Yan Ye
Qiang Na
Changtao Lao
Zhengchang Yang
Jun Jiang
Publication date: 01-12-2024
Publisher: BioMed Central
Keywords: Lumbar Disc Herniation
Lumbar Disc Herniation
Active Ingredients
Published in: Journal of Orthopaedic Surgery and Research / Issue 1/2024
Electronic ISSN: 1749-799X
DOI: https://doi.org/10.1186/s13018-024-04570-w

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Mechanism exploration of Osteoking in the treatment of lumbar disc herniation based on network pharmacology and molecular docking

Abstract

Objective

Methods

Results

Conclusions

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

Objective

Methods

Results

Conclusions

Please log in to get access to this content

Other articles of this Issue 1/2024

Glaucocalyxin A delays the progression of OA by inhibiting NF-κB and MAPK signaling pathways

The varying clinical effectiveness of single, three and five intraarticular injections of platelet-rich plasma in knee osteoarthritis

Bone cement reinforcement improves the therapeutic effects of screws in elderly patients with pelvic fragility factures

Clinical efficacy of greater trochanter osteotomy with tension wire fixation in total hip arthroplasty for Crowe type IV developmental dysplasia of the hip

Application direct anterior approach in pediatric femoral head and neck lesions

Antibacterial activities of titanium dioxide (TiO2) nanotube with planar titanium silver (TiAg) to prevent orthopedic implant infection