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BMC Complementary Medicine and Therapies

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Open Access 01-12-2019 | Research article

Effect of Osteoking on the osteogenic and adipogenic differentiation potential of rat bone marrow mesenchymal stem cells in vitro

Authors: Congtao Yu, Lifen Dai, Zhaoxia Ma, Hongbin Zhao, Yong Yuan, Yunfeng Zhang, Pengfei Bao, Yanfang Su, Daiping Ma, Change Liu, Xingfei Wu, Jinxue Liu, Yanjiao Li, Bing Wang, Min Hu

Published in: BMC Complementary Medicine and Therapies | Issue 1/2019

Abstract

Background

Bone damage is a condition that affects the quality of life of patients. Mesenchymal stem cells (MSCs) are important for bone repair. Osteoking is a natural compound in traditional Chinese Medicine used to treat bone diseases; however, the effect of Osteoking on the differentiation of MSCs has not been reported. In this study, we aimed to investigate the effect of Osteoking on the osteogenic and adipogenic differentiation potential of rat bone marrow mesenchymal stem cells (rbMSCs).

Methods

The effects of Osteoking on the proliferation and differentiation of rbMSCs were investigated. Different concentrations of Osteoking were prepared, and its cytotoxicity was evaluated by CCK-8 assay. The expression of osteogenic and adipogenic genes were determined, and several staining methods were used to reveal the osteogenic and adipogenic differentiation potential of rbMSCs.

Results

Our results show that appropriate concentrations of Osteoking can enhance osteogenic differentiation of rbMSCs and reduce adipogenic differentiation without any effect on proliferation. This may be related to the changes in related gene expression.

Conclusion

Osteoking enhances osteogenic differentiation and inhibits adipogenic differentiation of rbMSCs. Therefore, Osteoking may have a therapeutic potential for treating bone disease caused by changes in differentiation function of MSCs.

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Title: Effect of Osteoking on the osteogenic and adipogenic differentiation potential of rat bone marrow mesenchymal stem cells in vitro
Authors: Congtao Yu
Lifen Dai
Zhaoxia Ma
Hongbin Zhao
Yong Yuan
Yunfeng Zhang
Pengfei Bao
Yanfang Su
Daiping Ma
Change Liu
Xingfei Wu
Jinxue Liu
Yanjiao Li
Bing Wang
Min Hu
Publication date: 01-12-2019
Publisher: BioMed Central
Published in: BMC Complementary Medicine and Therapies / Issue 1/2019
Electronic ISSN: 2662-7671
DOI: https://doi.org/10.1186/s12906-019-2435-6

Keynote webinar | Spotlight on sleep in brain health

Springer Medicine

Effect of Osteoking on the osteogenic and adipogenic differentiation potential of rat bone marrow mesenchymal stem cells in vitro

Abstract

Background

Methods

Results

Conclusion

Keynote webinar | Spotlight on sleep in brain health

Springer Medicine

Abstract

Background

Methods

Results

Conclusion

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