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Lasers in Medical Science
Published in: Lasers in Medical Science 6/2016

01-08-2016 | Original Article

Low-level infrared laser modulates muscle repair and chromosome stabilization genes in myoblasts

Authors: Larissa Alexsandra da Silva Neto Trajano, Ana Carolina Stumbo, Camila Luna da Silva, Andre Luiz Mencalha, Adenilson S. Fonseca

Published in: Lasers in Medical Science | Issue 6/2016

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Abstract

Infrared laser therapy is used for skeletal muscle repair based on its biostimulative effect on satellite cells. However, shortening of telomere length limits regenerative potential in satellite cells, which occurs after each cell division cycle. Also, laser therapy could be more effective on non-physiologic tissues. This study evaluated low-level infrared laser exposure effects on mRNA expression from muscle injury repair and telomere stabilization genes in myoblasts in normal and stressful conditions. Laser fluences were those used in clinical protocols. C2C12 myoblast cultures were exposed to low-level infrared laser (10, 35, and 70 J/cm2) in standard or normal (10 %) and reduced (2 %) fetal bovine serum concentrations; total RNA was extracted for mRNA expression evaluation from muscle injury repair (MyoD and Pax7) and chromosome stabilization (TRF1 and TRF2) genes by real time quantitative polymerization chain reaction. Data show that low-level infrared laser increases the expression of MyoD and Pax7 in 10 J/cm2 fluence, TRF1 expression in all fluences, and TRF2 expression in 70 J/cm2 fluence in both 10 and 2 % fetal bovine serum. Low-level infrared laser increases mRNA expression from genes related to muscle repair and telomere stabilization in myoblasts in standard or normal and stressful conditions.
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Metadata
Title
Low-level infrared laser modulates muscle repair and chromosome stabilization genes in myoblasts
Authors
Larissa Alexsandra da Silva Neto Trajano
Ana Carolina Stumbo
Camila Luna da Silva
Andre Luiz Mencalha
Adenilson S. Fonseca
Publication date
01-08-2016
Publisher
Springer London
Published in
Lasers in Medical Science / Issue 6/2016
Print ISSN: 0268-8921
Electronic ISSN: 1435-604X
DOI
https://doi.org/10.1007/s10103-016-1956-1

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