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Gut Pathogens
Published in: Gut Pathogens 1/2022

01-12-2022 | Liver Cirrhosis | Research

Investigation and characterization of human gut phageome in advanced liver cirrhosis of defined etiologies

Authors: Mohadeseh Naseri, Fahimeh Palizban, Abbas Yadegar, Mohsen Khodarahmi, Hamid Asadzadeh Aghdaei, Hamidreza Houri, Javad Zahiri

Published in: Gut Pathogens | Issue 1/2022

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Abstract

Background

Liver cirrhosis is a major public health problem, accounting for high rates of morbidity and mortality worldwide. The cirrhosis etiology is a broad and essential step in planning a treatment strategy. Many recent studies have documented that gut microbiome alterations play a vital role in the development and progression of cirrhosis and its complications. Nevertheless, there is insufficient data on the correlation between liver cirrhosis and gut phageome alterations in patients with advanced liver diseases. This study aimed to analyze the taxonomic structure and functional attributes of the gut phageome in six different etiologies of advanced liver cirrhosis.

Methods

We first retrieved metagenomic sequencing data from three datasets of fecal samples taken from cirrhotic patients with various etiologies. Subsequently, several bioinformatics pipelines were used to analyze bacteriophage composition and determine their functionality.

Results

A gene catalog of 479,425 non-redundant genes was developed as a reference to measure gene prevalence. The results of the analysis revealed a few significant differences among the cohorts at the phage species level. However, the alternations were more evident as there were more in-depth analyses of the functional resolution of the gut phageome.

Conclusions

Our findings suggest that the functional analysis of the gut phageome would provide meaningful markers to predict the progression of liver cirrhosis and facilitate the development of novel treatment approaches.
Appendix
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Metadata
Title
Investigation and characterization of human gut phageome in advanced liver cirrhosis of defined etiologies
Authors
Mohadeseh Naseri
Fahimeh Palizban
Abbas Yadegar
Mohsen Khodarahmi
Hamid Asadzadeh Aghdaei
Hamidreza Houri
Javad Zahiri
Publication date
01-12-2022
Publisher
BioMed Central
Published in
Gut Pathogens / Issue 1/2022
Electronic ISSN: 1757-4749
DOI
https://doi.org/10.1186/s13099-022-00482-4

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