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Maxillofacial Plastic and Reconstructive Surgery

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Open Access 01-12-2018 | Research

The effects of proteins released from silk mat layers on macrophages

Authors: Ju-Won Kim, You-Young Jo, Hae Yong Kweon, Dae-Won Kim, Seong-Gon Kim

Published in: Maxillofacial Plastic and Reconstructive Surgery | Issue 1/2018

Abstract

Background

The objective of this study was to evaluate the changes in gene expression after incubation of cells with proteins released from different silk mat layers.

Methods

A silk cocoon from Bombyx mori was separated into four layers of equal thickness. The layers were numbered from 1 to 4 (from the inner to the outer layer). The proteins were released by sonication of a silk mat layer in normal saline. The concentration of proteins was determined by spectrophotometry. They were incubated with RAW264.7 cells, and changes in the expression of genes were evaluated by cDNA microarray analysis and quantitative reverse transcriptase-polymerase chain reaction (qRT-PCR).

Results

Layer 1 and 4 groups had higher protein concentrations compared to those in layer 2 and 3 groups. The genes associated with inflammation and angiogenesis showed significantly higher expression in layer 1 and 4 groups. The results of qRT-PCR were in agreement with those of the cDNA microarray analysis.

Conclusions

The silk mat from the middle portion of the silkworm cocoon yielded a lower protein release and caused an insignificant change in the expression of genes that are associated with inflammation and angiogenesis.

Seok H, Kim MK, Kim SG, Kweon H (2014) Comparison of silkworm-cocoon-derived silk membranes of two different thicknesses for guided bone regeneration. J Craniofac Surg 25:2066–2069CrossRefPubMed

Kim SG, Kim MK, Kweon H, Jo YY, Lee KG, Lee JK (2016) Comparison of unprocessed silk cocoon and silk cocoon middle layer membranes for guided bone regeneration. Maxillofac Plast Reconstr Surg 38:11CrossRefPubMedPubMedCentral

Kweon H, Jo YY, Seok H et al (2017) In vivo bone regeneration ability of different layers of natural silk cocoon processed using an eco-friendly method. Macromol Res 25:806–816CrossRef

Zhang Y, Zhao P, Dong Z et al (2015) Comparative proteome analysis of multi-layer cocoon of the silkworm, Bombyx mori. PLoS One 10:e0123403CrossRefPubMedPubMedCentral

Mondal M, Trivedy K, Kumar N (2007) The silk proteins, sericin and fibroin in silkworm, Bombyx mori Linn.—a review. Casp J Envir Sci 5:63–76

Ha Y, Park YW, Kweon H, Jo YY, Kim SG (2014) Comparison of the physical properties and in vivo bioactivities of silkworm-cocoon-derived silk membrane, collagen membrane, and polytetrafluoroethylene membrane for guided bone regeneration. Macromol Res 22:1018–1023CrossRef

Chen F, Porter D, Vollrath F (2012) Morphology and structure of silkworm cocoons. Mater Sci Eng C 32:772–778CrossRef

Mandal BB, Priya AS, Kundu SC (2009) Novel silk sericin/gelatin 3-D scaffolds and 2-D films: fabrication and characterization for potential tissue engineering applications. Acta Biomater 5:3007–3020CrossRefPubMed

Jo YY, Kweon H, Kim DW et al (2017) Bone regeneration is associated with the concentration of tumour necrosis factor-alpha induced by sericin released from a silk mat. Sci Rep 7:15589CrossRefPubMedPubMedCentral

10.

Kim JY, Choi JY, Jeong JH et al (2010) Low molecular weight silk fibroin increases alkaline phosphatase and type I collagen expression in MG63 cells. BMB Rep 43:52–56CrossRefPubMed

11.

Nayak S, Dey T, Naskar D, Kundu SC (2013) The promotion of osseointegration of titanium surfaces by coating with silk protein sericin. Biomaterials 34:2855–2864CrossRefPubMed

12.

Fujimoto Y, Fujita T, Kuramoto N et al (2018) The role of interleukin-19 in contact hypersensitivity. Biol Pharm Bull 41:182–189CrossRefPubMed

13.

Song JE, Park SJ, Lee KY, Lee WJ (2018) Amniotic fluid HIF1alpha and exosomal HIF1alpha in cervical insufficiency patients with physical examination-indicated cerclage. J Matern Fetal Neonatal Med. https://doi.org/10.1080/14767058.2018.1432037

14.

Vasilopoulos Y, Sourli F, Zafiriou E et al (2013) High serum levels of HIF-1alpha in psoriatic patients correlate with an over-expression of IL-6. Cytokine 62:38–39CrossRefPubMed

15.

Guo X, Zhu Z, Zhang W et al (2017) Nuclear translocation of HIF-1alpha induced by influenza A (H1N1) infection is critical to the production of proinflammatory cytokines. Emerg Microbes Infect 6:e39CrossRefPubMedPubMedCentral

16.

Bergers G, Benjamin LE (2003) Tumorigenesis and the angiogenic switch. Nat Rev Cancer 3:401–410CrossRefPubMed

17.

Jiang S, Li Y, Lin T et al (2016) IL-35 inhibits angiogenesis through VEGF/Ang2/Tie2 pathway in rheumatoid arthritis. Cell Physiol Biochem 40:1105–1116CrossRefPubMed

18.

Fuster-Matanzo A, Manferrari G, Marchetti B, Pluchino S (2018) Wnt3a promotes pro-angiogenic features in macrophages in vitro: implications for stroke pathology. Exp Biol Med 243:22–28CrossRef

19.

Yeo EJ, Cassetta L, Qian BZ et al (2014) Myeloid WNT7b mediates the angiogenic switch and metastasis in breast cancer. Cancer Res 74:2962–2973CrossRefPubMedPubMedCentral

20.

Khorshidi A, Dhaliwal P, Yang BB (2016) Noncoding RNAs in tumor angiogenesis. Adv Exp Med Biol 927:217–241CrossRefPubMed

21.

Gao SY, Zheng GS, Wang L et al (2017) Zoledronate suppressed angiogenesis and osteogenesis by inhibiting osteoclasts formation and secretion of PDGF-BB. PLoS One 12:e0179248CrossRefPubMedPubMedCentral

22.

Xie H, Cui Z, Wang L et al (2014) PDGF-BB secreted by preosteoclasts induces angiogenesis during coupling with osteogenesis. Nat Med 20:1270–1278CrossRefPubMedPubMedCentral

23.

Heldin CH, Westermark B (1999) Mechanism of action and in vivo role of platelet-derived growth factor. Physiol Rev 79:1283–1316CrossRefPubMed

Title: The effects of proteins released from silk mat layers on macrophages
Authors: Ju-Won Kim
You-Young Jo
Hae Yong Kweon
Dae-Won Kim
Seong-Gon Kim
Publication date: 01-12-2018
Publisher: Springer Berlin Heidelberg
Published in: Maxillofacial Plastic and Reconstructive Surgery / Issue 1/2018
Electronic ISSN: 2288-8586
DOI: https://doi.org/10.1186/s40902-018-0149-1

Keynote webinar | Spotlight on sleep in brain health

Springer Medicine

The effects of proteins released from silk mat layers on macrophages

Abstract

Background

Methods

Results

Conclusions

Keynote webinar | Spotlight on sleep in brain health

Springer Medicine

Abstract

Background

Methods

Results

Conclusions

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