Skip to main content
Key Specialties
Cardiology Diabetology Endocrinology Gastroenterology Geriatrics and Gerontology Gynecology and Obstetrics Hematology Infectious Disease Internal Medicine Nephrology Neurology Oncology Pediatrics Respiratory Medicine Rheumatology Surgery
Congress Coverage
2024 ACC Congress 2023 ESMO Congress 2023 EASD Congress 2023 ERS Congress 2023 ESC Congress 2023 EHA Congress 2023 ASCO Annual Meeting
Clinical Collections
Advances in Alzheimer’s

Keynote webinar | Spotlight on sleep in brain health

LIVE: Thursday 2nd May 2024, 18:00-19:30 (CEST)

Quality sleep is essential for health. But what happens to our brains when sleep patterns are disturbed? Join our experts to explore the interplay between sleep disruption and neurological diseases, and the questions that you need to be asking your patients to help you prevent the harmful effects of sleep deprivation.
ADVANCED SEARCH
Log in
Top
Clinical Phytoscience
Published in: Clinical Phytoscience 1/2018

Open Access 01-12-2018 | Original contribution

Phytochemical studies, antiangiogenic, anti-inflammatory and antioxidant activities of Scyphocephalium ochocoa Warb. (Myristicaceae), medicinal plant from Gabon

Authors: Rick-Leonid Ngoua-Meye-Misso, Jean De La Croix Ndong, Cédric Sima-Obiang, Joseph Privat Ondo, Guy Roger Ndong-Atome, Felix Ovono Abessolo, Louis-Clément Obame-Engonga

Published in: Clinical Phytoscience | Issue 1/2018

Login to get access

Abstract

Background

The search for new anti-cancer molecules is one of the main concerns of oncology researchers. Scyphocephalium ochocoa is a plant of Myristicaceae family, used in traditional medicine against inflammatory diseases and several types of cancer. It is well established that free radicals, chronic inflammation and angiogenesis play an important role in initiation, tumor progression and metastasis formation. The aim of this study was to carry out a phytochemical screening, to determine the phenolic compounds content, to investigate the antiangiogenic, anti-inflammatory and antioxidant activities of water, water-ethanol and ethanol extracts of S. ochocoa.

Methods

Phytochemical screening and determination of phenolic compounds content were performed using standard methods. Antiangiogenic activity was assessed using chick chorioallantoic membrane (CAM) model and Drabkin test. Anti-inflammatory activity was estimated by protein denaturation and erythrocyte membrane stabilization method. Finally the antioxidant activity was appreciated by DPPH radical inhibition and phosphomolybdenum assay.

Results

The results of phytochemical studies show that extracts of bark of S. ochocoa are rich in polyphenols, tannins, flavonoids, proantocyanidins, saponosides, flavonols, flavanonols, sterol and triterpenes. The water extract showed good antiangiogenic activity (IC50 = 1.153 μg/mL). Strong anti-inflammatory activity was observed with all extracts, IC50 ranging from 34.775 ± 2.543 μg/mL to 74.577 ± 3.456 μg/mL for protein denaturation inhibition test and IC50 values ranging from 36.793 ± 0.529 μg/mL at 48.912 ± 0.957 μg/mL for antihemolytic activity. In addition, all extracts showed good antioxidant activity marked by a strong inhibition of the DPPH radical (IC50 ranging from 4.969 ± 0.263 μg/mL to 16.188 ± 0.336 μg/mL and AAI ranging from 3.090 ± 0.065 to 10.080 ± 0.517) and by greater total antioxidant capacity (with contents ranging from 37.654 ± 0.995 to 131.302 ± 1.102 VtCE (mg)/g dry extract).

Conclusion

Ultimately, these results could justify the use of S. ochocoa extracts in traditional medicine in the treatment of diseases related to angiogenesis and cancer, inflammatory diseases and diseases due to oxidative stress. A phyto-product with such a pharmacological profile could be a good candidate for the development of anticancer.
Literature
1.
Letouzey R. Manuel de botanique forestière, Afrique Tropicale, Centre technique forestier d’Afrique tropicale Tome 2, Botanique Générale, 2è éd. Paris; 1972, 423.
2.
Walker R, Sillans S. Plantes utiles du Gabon. Ed Lechevalier. Sepia; 1961.
3.
Bouquet A. Féticheurs et médecines traditionnelles du congo (Brazzaville). Paris: Mémoires O.R.S.T.O.M. N°36; 1969.
4.
5.
Ferlay J, Shin HR, Bray F, Forman D, Mathers C, Parkin DM. Les estimations de la charge mondiale du cancer en 2008: Globocan 2008. Int J Cancer. 2010;127:2893–917.CrossRefPubMed
6.
7.
Liou GY, Storz P. Reactive oxygen species in cancer. Free Radic Res. 2010;44(5):1–31.CrossRef
8.
9.
10.
Balkwill F. Tumor necrosis factor or tumor promoting factor? Cytokine Growth Factor Rev. 2002;13:135–41.CrossRefPubMed
11.
Baron JA, Sandler RS. Nonsteroidal anti-inflammatory drugs and cancer prevention. Annu Rev Med. 2000;51:511–23.CrossRefPubMed
12.
Garcia-Rodriguez LA, Huerta-Alvarez C. Reduced risk of colorectal cancer among long-term users of aspirin and nonaspirin nonsteroidal antiinflammatory drugs. Epidemiology. 2001;12:88–93.CrossRefPubMed
13.
Rossi D, Zlotnik A. The biology of chemokines and their receptors. Annu Rev Immunol. 2000;18:217–42.CrossRefPubMed
14.
Folkman J, Shing Y. Angiogenesis. J Biol Chem. 1992;267(16):10931–4.PubMed
15.
16.
Ciulei, I. Methodology for Analysis of Vegetable Drugs. Practical Manual on the Industrial Utilisation of Medicinal and Aromatic Plants. Romania: Bucharest. 1982;1–62.
17.
Sofowora A. Medicinal plants and traditional. Ibadan Nigeria: Medicine in Africa Spectrum books Ltd; 1993. p. 289.
18.
Vernon LS, Orthofer R, Raventos LRM. Analysis of total phenols and other oxidation substrates and antioxidants by means of Folin-Ciocalteu reagent. Methods Enzymol. 1999;299:152–78.CrossRef
19.
Quettier-Deleu C, Gressier B, Vasseur J, Dine T, Brunet C. Phenolic compounds and antioxidant activities of buckwheat (Fagopyrum esculentum Moench) hulls and flour. J Ethnopharmacol. 2000;72:35–42.CrossRefPubMed
20.
21.
Prigent S. Interactions of phenolics compounds with globular proteins and their effects on food related functional properties. PhD Thesis, Wageningen University, Wageningen, The Netherlands. 2005; 131–133.
22.
Ribatti D, Nico B, Vacca A, Presta M. The gelatin sponge-chorioallantoic membrane assay. Nat Protoc. 2006;1(1):85–91.CrossRefPubMed
23.
Ribatti D. Chick embryo chorioallantoic membrane as a useful tool to study angiogenesis. Int Rev Cell Mol Biol. 2008;270:181–224.CrossRefPubMed
24.
Drabkin DL, Austin JH. Spectrophotometric studies II. Preparations from washed blood cells; nitric oxide hemoglobin and sulfhemoglobin. J Biol Chem. 1935;112:51–65.
25.
Yi J-M, Bang OS, Kim NS. An evaluation of the anti-angiogenic effect of the Korean medicinal formula Sa-mi-yeon-geon-tang in vitro and in ovo. BMC Complement Altern Med. 2015;15:42.CrossRefPubMedPubMedCentral
26.
Williams LAD, O'Connar A, Latore L, Dennis O, Ringer S, Whittaker JA, et al. The in vitro Anti-denaturation effects induced by natural products and non-steroidal compounds in heat treated (Immunogenic) Bovine Serum Albumin is proposed as a screening assay for the detection of anti-inflammatory compounds, without the use of animals, in the early stages of the drug discovery process. West Indian Med J. 2008;57(4):327–31.PubMed
27.
Chatterjee P, Chandra S, Dey P, Bhattacharya S. Evaluation of anti-inflammatory effects of green tea and black tea: a comparative in vitro study. J Adv Pharm Technol Res. 2012;3(2):136–8.CrossRefPubMedPubMedCentral
28.
Ngoua-Meye-Misso RL, Sima-Obiang C, Ndong JDLC, Ondo JP, Abessolo FO, Obame-Engonga LC. Phytochemical screening, antioxidant, anti-inflammatory and antiangiogenic activities of Lophira procera a. Chev. (Ochnaceae) medicinal plant from Gabon. Egyp Jour Bas App Sci. 2018;5(1):80–6.
29.
Shinde UA, Phadke AS, Nair AM, Mungantiwar AA, Dikshit VJ, Sarsf MN. Membrane stabilization activity a possible mechanism of action for the anti-inflammatory activity of Cedrus deodara wood oil. Fitoterapia. 1989;70:251–7.CrossRef
30.
Scherer R, Godoy HT. Antioxidant activity index (AAI) by 2,2-diphenyl- 1-picrylhydrazyl method. Food Chem. 2009;112:654–8.CrossRef
31.
Kubola J, Siriamornpun S. Phenolic content and antioxidant activities of bitter gourd (Momordica charantia L.) leaf stem and fruit fraction extracts in vitro. Food Chem. 2008;110:881–90.CrossRefPubMed
32.
Ngoua-Meye-Misso RL, Ondo JP, Assam EJN, Orango BJO, Sima-Obiang C, Ndong JDLC, et al. Phytochemical screening, antioxidant and antiangiogenic properties of Oncoba welwitschii (Oliv.) Gilgn. And Tetrorchidium oppositifolium (Pax. And Khoffm.), medicinal plants from Gabon. Int J Innov Res Sci Eng Technol. 2017;6:1–10.
33.
Lacaille-Dubois MA, Wagner H. Importance pharmacologique des dérivés polyphénoliques. Acta Botanica Gallica. 1996;143(6):555–62.CrossRef
34.
Opie-EL. On the relation of necrosis and inflammation to denaturation of proteins. J Exp Med 1962; 115(3): 597–608.
35.
36.
Mounnissamy VM, Kavimani S, Balu V, Quine SD. Evaluation of anti-inflammatory and membrane stabilizing properties of ethanol extract of Cansjera reheedii J.Gmelin (Opiliaceae). Iranian J Pharmacol Therapeut. 2007;6(2):235–0.
37.
Feirrali M, Signormi C, Ciccolili L, Comporti M. Iron release and membrane damage in erythrocytes exposed to oxidizing agents, phenylhydrazine, devicene and iso-uranil. Biochem J. 1992;285:295–301.CrossRef
38.
Feuya TGR, Foundikou H, Lebibi J, Choudhary MI, Menkem EZ, Nantia EA, et al. Phytochemical analysis, antioxidant and antimicrobial properties of the leaves and stem bark of Scyphocephalium ochocoa Warb (Myristicaceae). J Pharmacogn Nat Prod. 2015;1:111.
39.
Sima-Obiang C, Ngoua- Meye-Misso RL, Ndong-Atome GR, Ondo JP, Obame-Engonga LC, Nsi-Emvo E. Chemical composition, antioxidant and antimicrobial activities of stem barks of Englerina gabonensis Englerand Sterculia tragacantha Lindlfrom Gabon. Int J Phytomedicine. 2017;9(3):501–10.CrossRef
40.
Obame-Engonga LC, Abdoul-Latif-Fatouma M, Ondo JP, Sima-Obiang C, Ngoua-Meye-Misso RL, Traoré A, et al. phytochemical screening, antioxidant and antibacterial activities of Guibourtia ehie and Syzygium rowlandii medicinal plants from Gabon. Int J Curr Res. 2017;9(8):56354–60.
41.
Obame-Engonga LC, Sima-Obiang C, Ngoua-Meye-Misso RL, Orango BJO, Ondo JP, Ndong-Atome GR et al. Larvicidal and ovicidal properties against Anopheles gambiae, antioxidant and antibacterial activities of the combination of essential oils Eucalyptus citriodora, Cymbopogon giganteus and Cymbopogon nardus from Gabon. J Multidiscip Eng Sci Technol 2017; 4(8): 7887–7894.
42.
Metadata
Title
Phytochemical studies, antiangiogenic, anti-inflammatory and antioxidant activities of Scyphocephalium ochocoa Warb. (Myristicaceae), medicinal plant from Gabon
Authors
Rick-Leonid Ngoua-Meye-Misso
Jean De La Croix Ndong
Cédric Sima-Obiang
Joseph Privat Ondo
Guy Roger Ndong-Atome
Felix Ovono Abessolo
Louis-Clément Obame-Engonga
Publication date
01-12-2018
Publisher
Springer Berlin Heidelberg
Published in
Clinical Phytoscience / Issue 1/2018
Electronic ISSN: 2199-1197
DOI
https://doi.org/10.1186/s40816-018-0075-x

Other articles of this Issue 1/2018

Clinical Phytoscience 1/2018 Go to the issue

Original contribution

Evaluation of central nervous system (CNS) depressant activity of methanolic extract of Commelina diffusa Burm. in mice

Original contribution

Assessment of antimicrobial and immunomodulatory activities of termite associated fungi, Termitomyces clypeatus R. Heim (Lyophyllaceae, Basidiomycota)

Original contribution

Screening of phytochemical and pharmacological activities of Syzygium caryophyllatum (L.) Alston

Original contribution

Phytochemical, anti-inflammatory, anti-ulcerogenic and hypoglycemic activities of Periploca angustifolia L extracts in rats

Original contribution

Anthelmintic activity of Piper sylvaticum Roxb. (family: Piperaceae): In vitro and in silico studies

Original contribution

Evaluation of aphrodisiac activity of ethanol extract of Ganoderma lucidum in male Wistar rats