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Open Access 01-12-2016 | Short report

Effects of Escherichia coli Nissle 1917 and Ciprofloxacin on small intestinal epithelial cell mRNA expression in the neonatal piglet model of human rotavirus infection

Authors: Francine C. Paim, Stephanie N. Langel, David D. Fischer, Sukumar Kandasamy, Lulu Shao, Moyasar A. Alhamo, Huang-Chi Huang, Anand Kumar, Gireesh Rajashekara, Linda J. Saif, Anastasia N. Vlasova

Published in: Gut Pathogens | Issue 1/2016

Abstract

We evaluated the effects of the probiotic Escherichia coli Nissle 1917 (EcN) and the antibiotic Ciprofloxacin (Cipro) on mRNA expression of intestinal epithelial cells (IEC) in gnotobiotic (Gn) piglets colonized with a defined commensal microflora (DMF) and inoculated with human rotavirus (HRV) that infects IECs. We analyzed mRNA levels of IEC genes for enteroendocrine cells [chromogranin A (CgA)], goblet cells [mucin 2 (MUC2)], transient amplifying progenitor cell [proliferating cell nuclear antigen (PCNA)], intestinal epithelial stem cell (SOX9) and enterocytes (villin). Cipro treatment enhanced HRV diarrhea and decreased the mRNA levels of MUC2 and villin but increased PCNA. These results suggest that Cipro alters the epithelial barrier, potentially decreasing the numbers of mature enterocytes (villin) and goblet cells secreting protective mucin (MUC2). These alterations may induce increased IEC proliferation (PCNA expression) to restore the integrity of the epithelial layer. Coincidental with decreased diarrhea severity in EcN treated groups, the expression of CgA and villin was increased, while SOX9 expression was decreased representing higher epithelial integrity indicative of inhibition of cellular proliferation. Thus, EcN protects the intestinal epithelium from damage by increasing the gene expression of enterocytes and enteroendocrine cells, maintaining the absorptive function and, consequently, decreasing the severity of diarrhea in HRV infection.

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Title: Effects of Escherichia coli Nissle 1917 and Ciprofloxacin on small intestinal epithelial cell mRNA expression in the neonatal piglet model of human rotavirus infection
Authors: Francine C. Paim
Stephanie N. Langel
David D. Fischer
Sukumar Kandasamy
Lulu Shao
Moyasar A. Alhamo
Huang-Chi Huang
Anand Kumar
Gireesh Rajashekara
Linda J. Saif
Anastasia N. Vlasova
Publication date: 01-12-2016
Publisher: BioMed Central
Published in: Gut Pathogens / Issue 1/2016
Electronic ISSN: 1757-4749
DOI: https://doi.org/10.1186/s13099-016-0148-7

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Effects of Escherichia coli Nissle 1917 and Ciprofloxacin on small intestinal epithelial cell mRNA expression in the neonatal piglet model of human rotavirus infection

Abstract

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