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Gut Pathogens
Published in: Gut Pathogens 1/2016

Open Access 01-12-2016 | Research

Genotyping and antibiotic resistance of thermophilic Campylobacter isolated from chicken and pig meat in Vietnam

Authors: Tuan Ngoc Minh Nguyen, Helmut Hotzel, Hosny El-Adawy, Hanh Thi Tran, Minh Thi Hong Le, Herbert Tomaso, Heinrich Neubauer, Hafez Mohamed Hafez

Published in: Gut Pathogens | Issue 1/2016

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Abstract

Background

Campylobacter species are recognized as the most common cause of foodborne bacterial gastroenteritis in humans. In this study nine Campylobacter strains isolated from chicken meat and pork in Hanoi, Vietnam, were characterized using molecular methods and tested for antibiotic resistance.

Results

The nine isolates (eight C. jejuni and one C. coli) were identified by multiplex PCR, and tested for the presence or absence of 29 gene loci associated with virulence, lipooligosaccharide (LOS) biosynthesis and further functions. flaA typing, multilocus sequence typing and microarray assay investigation showed a high degree of genetic diversity among these isolates. In all isolates motility genes (flaA, flaB, flhA, fliM), colonization associated genes (cadF, docB), toxin production genes (cdtA, cdtB, secD, secF), and the LOS biosynthesis gene pglB were detected. Eight gene loci (fliY, virB11, Cje1278, Cj1434c, Cj1138, Cj1438c, Cj1440c, Cj1136) could not be detected by PCR. A differing presence of the gene loci ciaB (22.2 %), Cje1280 (77.8 %), docC (66.7 %), and cgtB (55.6 %) was found. iamA, cdtC, and the type 6 secretion system were present in all C. jejuni isolates but not in C. coli. flaA typing resulted in five different genotypes within C. jejuni, MLST classified the isolates into seven sequence types (ST-5155, ST-6736, ST-2837, ST-4395, ST-5799, ST-4099 and ST-860). The microarray assay analysis showed a high genetic diversity within Vietnamese Campylobacter isolates which resulted in eight different types for C. jejuni. Antibiotic susceptibility profiles showed that all isolates were sensitive to gentamicin and most isolates (88.8 %) were sensitive to chloramphenicol, erythromycin and streptomycin. Resistance rates to nalidixic acid, tetracycline and ciprofloxacin were 88.9, 77.8 and 66.7 %, respectively.

Conclusions

To the best of our knowledge, this study is the first report that shows high genetic diversity and remarkable antibiotic resistance of Campylobacter strains isolated from meat in Vietnam which can be considered of high public health significance. These preliminary data show that large scale screenings are justified to assess the relevance of Campylobacter infections on human health in Vietnam.
Literature
1.
Allos BM. Campylobacter jejuni infections: update on emerging issues and trends. Clin Infect Dis. 2001;32:1201–6.CrossRefPubMed
2.
3.
Dingle KE, Colles FM, Wareing DR, Ure R, Fox AJ, Bolton FE, et al. Multilocus sequence typing system for Campylobacter jejuni. J Clin Microbiol. 2001;39:14–23.CrossRefPubMedPubMedCentral
4.
Hafez HM. Bacterial contaminations and risks from poultry meat and eggs. Arch Geflüglk. 2003;67:146–52.
5.
EFSA. The European Union summary report on trends and sources of zoonoses, zoonotic agents and food-borne outbreaks in 2012. EFSA J. 2015;12:3547.
6.
Weijtens MJ, van der Plas J, Bijker PG, Urlings HA, Koster D, van Logtestijn JG, et al. The transmission of Campylobacter in piggeries; an epidemiological study. J Appl Microbiol. 1997;83:693–8.CrossRefPubMed
7.
Gill CO, Badoni M, McGinnis JC. Assessment of the adequacy of cleaning of equipment used for breaking beef carcasses. Int J Food Microbiol. 1999;46:1–8.CrossRefPubMed
8.
Gill CO, McGinnis JC, Bryant J. Contamination of beef chucks with Escherichia coli during carcass breaking. J Food Prot. 2001;64:1824–7.PubMed
9.
Duffy EA, Belk KE, Sofos JN, Bellinger GR, Pape A, Smith GC. Extent of microbial contamination in United States pork retail products. J Food Prot. 2001;64:172–8.PubMed
10.
Pearce RA, Wallace FM, Call JE, Dudley RL, Oser A, Yoder L, et al. Prevalence of Campylobacter within a swine slaughter and processing facility. J Food Prot. 2003;66:1550–6.PubMed
11.
Coker RJ, Hunter BM, Rudge JW, Liverani M, Hanvoravongchai P. Emerging infectious diseases in Southeast Asia: regional challenges to control. Lancet. 2011;377:599–609.CrossRefPubMed
12.
Bodhidatta L, Lan NT, Hien BT, Lai NV, Srijan A, Serichantalergs O, et al. Rotavirus disease in young children from Hanoi, Vietnam. Pediatr Infect Dis J. 2007;26:325–8.CrossRefPubMed
13.
My PV, Thompson C, Phuc HL, Tuyet PT, Vinh H, Hoang NV, et al. Endemic norovirus infections in children, Ho Chi Minh City, Vietnam, 2009–2010. Emerg Infect Dis. 2013;19:977–80.CrossRefPubMedPubMedCentral
14.
Trang DT, Hien BTT, Molbak K, Cam PD, Dalsgaard A. Epidemiology and aetiology of diarrhoeal diseases in adults engaged in wastewater-fed agriculture and agriculture in Hanoi, Vietnam. Trop Med Int Health. 2007;12(Suppl. 2):23–33.CrossRef
15.
Isenbarger DW, Hien BT, Ha HT, Ha TT, Bodhidatta L, Pang LW, et al. Prospective study of the incidence of diarrhoea and prevalence of bacterial pathogens in a cohort of Vietnamese children along the Red River. Epidemiol Infect. 2001;127:229–36.CrossRefPubMedPubMedCentral
16.
17.
Huong LQ, Hanh TT, Cam PD, Be NT. Study on the prevalence of Campylobacter spp. from chicken meat in Hanoi, Vietnam. Ann N Y Acad Sci. 2006;1081:273–5.CrossRef
18.
Ha TA, Pham T. Study of Salmonella, Campylobacter, and Escherichia coli contamination in raw food available in factories, schools, and hospital canteens in Hanoi, Vietnam. Ann N Y Acad Sci. 2006;1081:262–5.CrossRefPubMed
19.
Schwan P. Prevalence and antibiotic resistance of Campylobacter spp. in poultry and raw meat in the Can Tho Province, Vietnam. Examensarbete. 2010;49. ISSN 1652-8697. http://​epsilon.​slu.​se.
20.
Garin B, Gouali M, Wouafo M, Perchec AM, Tham MT, Ravaonindrina N, et al. Prevalence, quantification and antimicrobial resistance of Campylobacter spp. on chicken neck-skins at points of slaughter in 5 major cities located on 4 continents. Int J Food Microbiol. 2012;157:102–7.CrossRefPubMed
21.
Carrique-Mas JJ, Bryant JE, Cuong NV, Hoang NV, Campbell J, Hoang NV, et al. An epidemiological investigation of Campylobacter in pig and poultry farms in the Mekong delta of Vietnam. Epidemiol Infect. 2014;142:1425–36.CrossRefPubMedPubMedCentral
22.
Bao VN, Fries R, Zessin KH, Kyule MN, Pinthong R, Baumann MPO. Salmonella and Campylobacter in broiler carcasses in Vietnam. In: Proceedings of the 11th international symposium veterinary epidemiology and economics. 2006. www.​sciquest.​org.​nz.
23.
Eberle KN, Kiess AS. Phenotypic and genotypic methods for typing Campylobacter jejuni and Campylobacter coli in poultry. Poult Sci. 2012;91:255–64.CrossRefPubMed
24.
El-Adawy H, Hotzel H, Tomaso H, Neubauer H, Taboada EN, Ehricht R, et al. Detection of genetic diversity in Campylobacter jejuni isolated from a commercial turkey flock using flaA typing, MLST analysis and microarray assay. PLoS One. 2013;8:e51582.CrossRefPubMedPubMedCentral
25.
El-Adawy H, Hotzel H, Tomaso H, Neubauer H, Hafez HM. Elucidation of colonization time and prevalence of thermophilic Campylobacter species during turkey rearing using multiplex polymerase chain reaction. Poult Sci. 2012;91:454–9.CrossRefPubMed
26.
Parkhill J, Wren BW, Mungall K, Ketley JM, Churcher C, Basham D, et al. The genome sequence of the food-borne pathogen Campylobacter jejuni reveals hypervariable sequences. Nature. 2000;403:665–8.CrossRefPubMed
27.
Bang DD, Nielsen EM, Scheutz F, Pedersen K, Handberg K, Madsen M. PCR detection of seven virulence and toxin genes of Campylobacter jejuni and Campylobacter coli isolates from Danish pigs and cattle and cytolethal distending toxin production of the isolates. J Appl Microbiol. 2003;94:1003–14.CrossRefPubMed
28.
Hänel I, Borrmann E, Müller J, Alter T. Relationships between bacterial genotypes and in vitro virulence properties of Campylobacter jejuni and Campylobacter coli isolated from turkeys. J Appl Microbiol. 2007;102:433–41.CrossRefPubMed
29.
Müller J, Schulze F, Müller W, Hänel I. PCR detection of virulence-associated genes in Campylobacter jejuni strains with differential ability to invade Caco-2 cells and to colonize the chick gut. Vet Microbiol. 2006;113:123–9.CrossRefPubMed
30.
Nachamkin I, Yang XH, Stern NJ. Role of Campylobacter jejuni flagella as colonization factors for three-day-old chicks: analysis with flagellar mutants. Appl Environ Microbiol. 1993;59:1269–73.PubMedPubMedCentral
31.
Hänel I, Schulze F, Hotzel H, Schubert E. Detection and characterization of two cytotoxins produced by Campylobacter jejuni strains. Zentralbl Bakteriol. 1998;288:131–43.CrossRefPubMed
32.
Whitehouse CA, Balbo PB, Pesci EC, Cottle DL, Mirabito PM, Pickett CL. Campylobacter jejuni cytolethal distending toxin causes a G2-phase cell cycle block. Infect Immun. 1998;66:1934–40.PubMedPubMedCentral
33.
Pickett CL, Pesci EC, Cottle DL, Russell G, Erdem AN, Zeytin H. Prevalence of cytolethal distending toxin production in Campylobacter jejuni and relatedness of Campylobacter sp. cdtB gene. Infect Immun. 1996;64:2070–8.PubMedPubMedCentral
34.
Jain D, Prasad KN, Sinha S, Husain N. Differences in virulence attributes between cytolethal distending toxin positive and negative Campylobacter jejuni strains. J Med Microbiol. 2008;57:267–72.CrossRefPubMed
35.
Lee RB, Hassane DC, Cottle DL, Pickett CL. Interactions of Campylobacter jejuni cytolethal distending toxin subunits CdtA and CdtC with HeLa cells. Infect Immun. 2003;71:4883–90.CrossRefPubMedPubMedCentral
36.
Lara-Tejero M, Galán JE. CdtA, CdtB, and CdtC form a tripartite complex that is required for cytolethal distending toxin activity. Infect Immun. 2001;69:4358–65.CrossRefPubMedPubMedCentral
37.
Bacon DJ, Alm RA, Hu L, Hickey TE, Ewing CP, Batchelor RA, et al. DNA sequence and mutational analyses of the pVir plasmid of Campylobacter jejuni 81-176. Infect Immun. 2002;70:6242–50.CrossRefPubMedPubMedCentral
38.
Bacon DJ, Alm RA, Burr DH, Hu L, Kopecko DJ, Ewing CP, et al. Involvement of a plasmid in virulence of Campylobacter jejuni 81-176. Infect Immunol. 2000;68:4384–90.CrossRef
39.
Harrison JW, Dung TT, Siddiqui F, Korbrisate S, Bukhari H, Tra MP, et al. Identification of possible virulence marker from Campylobacter jejuni isolates. Emerg Infect Dis. 2014;20:1026–9.CrossRefPubMedPubMedCentral
40.
Isenbarger DW, Hoge CW, Srijan A, Pitarangsi C, Vithayasai N, Bodhidatta L, et al. Comparative antibiotic resistance of diarrheal pathogens from Vietnam and Thailand, 1996–1999. Emerg Infect Dis. 2002;8:175–80.CrossRefPubMedPubMedCentral
41.
Clinical and Laboratory Standards Institute (CLSI). Performance standards for antimicrobial disk and dilution susceptibility tests for bacterial isolates from animals; approved standards. 3rd ed. M31-A3, vol. 28, no. 8; 2008.
42.
Luber P, Bartelt E, Genschow E, Wagner J, Hahn H. Comparison of broth microdilution, E test, and agar dilution methods for antibiotic susceptibility testing of Campylobacter jejuni and Campylobacter coli. J Clin Microbiol. 2003;41:1062–8.CrossRefPubMedPubMedCentral
43.
44.
International Standards Organization: [ISO] 10272-1. Microbiology of food and animal feeding stuffs—horizontal method for detection and enumeration of Campylobacter spp. Part 1: detection method. 2006.
45.
Taboada EN, Luebbert CC, Nash JH. Studying bacterial genome dynamics using microarray-based comparative genomic hybridization. Methods Mol Biol. 2007;396:223–53.CrossRefPubMed
46.
Müller J, Meyer B, Hänel I, Hotzel H. Comparison of lipooligosaccharide biosynthesis genes of Campylobacter jejuni strains with varying abilities to colonize the chicken gut and to invade Caco-2 cells. J Med Microbiol. 2007;56:1589–94.CrossRefPubMed
47.
Alonso R, Mateo E, Churruca E, Martinez I, Girbau C, Fernandez-Astorga A. MAMA-PCR assay for the detection of point mutations associated with high-level erythromycin resistance in Campylobacter jejuni and Campylobacter coli strains. J Microbiol Methods. 2005;63:99–103.CrossRefPubMed
48.
Payot S, Bolla JM, Corcoran D, Fanning S, Megraud F, Zhang Q. Mechanisms of fluoroquinolone and macrolide resistance in Campylobacter spp. Microbes Infect. 2006;8:1967–71.CrossRefPubMed
49.
Zirnstein G, Li Y, Swaminathan B, Angulo F. Ciprofloxacin resistance in Campylobacter jejuni isolates: detection of gyrA resistance mutations by mismatch amplification mutation assay PCR and DNA sequence analysis. J Clin Microbiol. 1999;37:3276–80.PubMedPubMedCentral
50.
Zirnstein G, Helsel L, Li Y, Swaminathan B, Besser J. Characterization of gyrA mutations associated with fluoroquinolone resistance in Campylobacter coli by DNA sequence analysis and MAMA PCR. FEMS Microbiol Lett. 2000;190:1–7.CrossRefPubMed
51.
Mazi W, Senok A, Al-Mahmeed A, Arzese A, Bindayna K, Botta G. Trends in antibiotic sensitivity pattern and molecular detection of tet(O)-mediated tetracycline resistance in Campylobacter jejuni isolates from human and poultry sources. Jpn J Infect Dis. 2008;61:82–4.PubMed
52.
El-Adawy H, Hotzel H, Düpre S, Tomaso H, Neubauer H, Hafez HM. Determination of antimicrobial sensitivities of Campylobacter jejuni isolated from commercial turkey farms in Germany. Avian Dis. 2012;56:685–92.CrossRefPubMed
53.
BVL: Berichte zur Lebensmittelsicherheit 2013, Zoonosen-Monitoring. ISBN 978-3-319-15379-7, 2015; 22.
54.
Buchanan CJ, Yoshida C, Kruczkiewicz P, Mutschall SK, Ross SI, Chu J, et al. High-resolution comparative genomic fingerprinting (CGF) of C. jejuni using ArrayTube™ miniaturized diagnostic microarrays. In: Proceedings of the 16th internnational workshop on Campylobacter, Helicobacter, and related organisms (CHRO 2011) 2011, Vancouver, Canada, p. 164.
55.
Krutkiewicz A, Klimuszko D. Genotyping and PCR detection of potential virulence genes in Campylobacter jejuni and Campylobacter coli isolates from different sources in Poland. Folia Microbiol (Praha). 2010;55:167–75.CrossRef
56.
Wassenaar TM, Bleumink-Pluym NM, Newell DG, Nuijten PJ, van der Zeijst BA. Differential flagellin expression in a flaA flaB+ mutant of Campylobacter jejuni. Infect Immun. 1994;62:3901–6.PubMedPubMedCentral
57.
Carvalho ACT, Ruiz-Palacios GM, Ramos-Cervantes P, Cervantes LE, Jiang X, Pickering LK. Molecular characterization of invasive and noninvasive Campylobacter jejuni and Campylobacter coli isolates. J Clin Microbiol. 2001;39:1353–9.CrossRefPubMedPubMedCentral
58.
Konkel ME, Garvis SG, Tipton SL, Anderson DE Jr, Cieplak W Jr. Identification and molecular cloning of a gene encoding a fibronectin-binding protein (CadF) from Campylobacter jejuni. Mol Microbiol. 1997;24:953–63.CrossRefPubMed
59.
Fouts DE, Mongodin EF, Mandrell RE, Miller WG, Rasko DA, Ravel J, et al. Major structural differences and novel potential virulence mechanisms from the genomes of multiple Campylobacter species. PLoS Biol. 2005;3:e15.CrossRefPubMedPubMedCentral
60.
Rozynek E, Dzierzanowska-Fangrat K, Jozwiak P, Popowski J, Korsak D, Dzierzanowska D. Prevalence of potential virulence markers in Polish Campylobacter jejuni and Campylobacter coli isolates obtained from hospitalized children and from chicken carcasses. J Med Microbiol. 2005;54:615–9.CrossRefPubMed
61.
González-Hein G, Huaracan B, Garcia P, Figueroa G. Prevalence of virulence genes in strains of Campylobacter jejuni isolated from human, bovine and broiler. Braz J Microbiol. 2014;44:1223–9.CrossRefPubMedPubMedCentral
62.
Van Deun K, Haesebrouck F, Heyndrickx M, Favoreel H, Dewulf J, Ceelen L, et al. Virulence properties of Campylobacter jejuni isolates of poultry and human origin. J Med Microbiol. 2007;56:1284–9.CrossRefPubMed
63.
Rizal A, Kumar A, Vidyarthi AS. Prevalence of pathogenic genes in Campylobacter jejuni isolated from poultry and human. Internet J Food Saf. 2010;12:29–34.
64.
Gu W, Siletzky RM, Wright S, Islam M, Kathariou S. Antimicrobial susceptibility profiles and strain type diversity of Campylobacter jejuni isolates from turkeys in eastern North Carolina. Appl Environ Microbiol. 2009;75:474–82.CrossRefPubMedPubMedCentral
65.
Miflin JK, Templeton JM, Blackall PJ. Antibiotic resistance in Campylobacter jejuni and Campylobacter coli isolated from poultry in the South-East Queensland region. J Antimicrob Chemother. 2007;59:775–8.CrossRefPubMed
66.
Luber P, Wagner J, Hahn H, Bartelt E. Antimicrobial resistance in Campylobacter jejuni and Campylobacter coli strains isolated in 1991 and 2001–2002 from poultry and humans in Berlin, Germany. Antimicrob Agents Chemother. 2003;47:3825–30.CrossRefPubMedPubMedCentral
67.
Luangtongkum T, Jeon B, Han J, Plummer P, Logue CM, Zhang Q. Antibiotic resistance in Campylobacter: emergence, transmission and persistence. Future Microbiol. 2009;4:189–200.CrossRefPubMedPubMedCentral
68.
Luangtongkum T, Morishita TY, Ison AJ, Huang S, McDermott PF, Zhang Q. Effect of conventional and organic production practices on the prevalence and antimicrobial resistance of Campylobacter spp. in poultry. Appl Environ Microbiol. 2006;72:3600–7.CrossRefPubMedPubMedCentral
69.
Hariharan H, Sharma S, Chikweto A, Matthew V, DeAllie C. Antimicrobial drug resistance as determined by the E-test in Campylobacter jejuni, C. coli, and C. lari isolates from the ceca of broiler and layer chickens in Grenada. Comp Immunol Microbiol Infect Dis. 2009;32:21–8.CrossRefPubMed
70.
Luangtongkum T, Moroshita TY, El-Tayeb AB, Ison AJ, Zhang Q. Comparison of antimicrobial susceptibility testing of Campylobacter spp. by the agar dilution and the agar disk diffusion methods. J Clin Microbiol. 2007;45:590–4.CrossRefPubMedPubMedCentral
71.
Andersen SR, Saadbye P, Shukri NM, Rosenquist H, Nielsen NL, Boel J. Antimicrobial resistance among Campylobacter jejuni isolated from raw poultry meat at retail level in Denmark. Int J Food Microbiol. 2006;107:250–5.CrossRefPubMed
72.
Andersen SR, Shukri NM, Boel J, Saadbye P. Metronidazole resistance in Campylobacter jejuni from poultry meat. J Food Prot. 2006;69:932–4.PubMed
73.
Charvalos E, Peteinaki E, Spyridaki I, Manetas S, Tselentis Y. Detection of ciprofloxacin resistance mutations in Campylobacter jejuni gyrA by nonradioisotopic single-strand conformation polymorphism and direct DNA sequencing. J Clin Lab Anal. 1996;10:129–33.CrossRefPubMed
74.
Ruiz J, Goni P, Marco F, Gallardo F, Mirelis B, Jimenez De Anta T, et al. Increased resistance to quinolones in Campylobacter jejuni: a genetic analysis of gyrA gene mutations in quinolone-resistant clinical isolates. Microbiol Immunol. 1998;42:223–6.CrossRefPubMed
75.
Wang Y, Huang WM, Taylor DE. Cloning and nucleotide sequence of the Campylobacter jejuni gyrA gene and characterization of quinolone resistance mutations. Antimicrob Agents Chemother. 1993;37:457–63.CrossRefPubMedPubMedCentral
76.
Wardak S, Szych J, Cieślik A. PCR-restriction fragment length polymorphism assay (PCR-RFLP) as an useful tool for detection of mutation in gyrA gene at 86-THR position associated with fluoroquinolone resistance in Campylobacter jejuni. Med Dosw Mikrobiol. 2005;57:295–301.PubMed
77.
Hänel I, Borrmann E, Müller J, Müller W, Pauly B, Liebler-Tenorio EM, et al. Genomic and phenotypic changes of Campylobacter jejuni strains after passage of the chicken gut. Vet Microbiol. 2009;136:121–9.CrossRefPubMed
78.
Karlyshev AV, Linton D, Gregson NA, Wren BW. A novel paralogous gene family involved in phase-variable flagella-mediated motility in Campylobacter jejuni. Microbiology. 2002;148:473–80.CrossRefPubMed
Metadata
Title
Genotyping and antibiotic resistance of thermophilic Campylobacter isolated from chicken and pig meat in Vietnam
Authors
Tuan Ngoc Minh Nguyen
Helmut Hotzel
Hosny El-Adawy
Hanh Thi Tran
Minh Thi Hong Le
Herbert Tomaso
Heinrich Neubauer
Hafez Mohamed Hafez
Publication date
01-12-2016
Publisher
BioMed Central
Published in
Gut Pathogens / Issue 1/2016
Electronic ISSN: 1757-4749
DOI
https://doi.org/10.1186/s13099-016-0100-x

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