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Journal of Experimental & Clinical Cancer Research
Published in: Journal of Experimental & Clinical Cancer Research 1/2018

Open Access 01-12-2018 | Research

CPNE1 is a target of miR-335-5p and plays an important role in the pathogenesis of non-small cell lung cancer

Authors: Haicheng Tang, Jianjie Zhu, Wenwen Du, Shunlin Liu, Yuanyuan Zeng, Zongli Ding, Yang Zhang, Xueting Wang, Zeyi Liu, Jianan Huang

Published in: Journal of Experimental & Clinical Cancer Research | Issue 1/2018

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Abstract

Background

Despite advances in diagnosis and treatment, the survival of non-small cell lung cancer (NSCLC) patients remains poor. There is therefore a strong need to identify potential molecular targets for the treatment of NSCLC. In the present study, we investigated the function of CPNE1 in the regulation of cell growth, migration and invasion.

Methods

Quantitative real-time PCR (qRT-PCR) was used to detect the expression of CPNE1 and miR-335-5p. Western blot and immunohistochemical assays were used to investigate the levels of CPNE1 and other proteins. Flow cytometry was used to determine cell cycle stage and apoptosis. CCK-8 and clonogenic assays were used to investigate cell proliferation. Wound healing, migration and invasion assays were used to investigate the motility of cells. A lung carcinoma xenograft mouse model was used to investigate the in vivo effects of CPNE1 overexpression.

Results

We observed that knockdown of CPNE1 and increased expression of miR-335-5p inhibits cell proliferation and motility in NSCLC cells, and found that CPNE1 was a target of miR-335-5p. In addition, our data indicated that CPNE1 inhibition could improve the clinical effects of EGFR-tyrosine kinase inhibitors.

Conclusions

The present results indicate that CPNE1 may be a promising molecular target in the treatment of NSCLC.
Appendix
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Metadata
Title
CPNE1 is a target of miR-335-5p and plays an important role in the pathogenesis of non-small cell lung cancer
Authors
Haicheng Tang
Jianjie Zhu
Wenwen Du
Shunlin Liu
Yuanyuan Zeng
Zongli Ding
Yang Zhang
Xueting Wang
Zeyi Liu
Jianan Huang
Publication date
01-12-2018
Publisher
BioMed Central
Published in
Journal of Experimental & Clinical Cancer Research / Issue 1/2018
Electronic ISSN: 1756-9966
DOI
https://doi.org/10.1186/s13046-018-0811-6

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