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Open Access 01-12-2015 | Letter to the Editor

Establishment and genetic characterization of a novel mixed-phenotype acute leukemia cell line with EP300-ZNF384 fusion

Authors: Nana Ping, Huiying Qiu, Qian Wang, Haiping Dai, Changgeng Ruan, Stefan Ehrentraut, Hans G. Drexler, Roderick A. F. MacLeod, Suning Chen

Published in: Journal of Hematology & Oncology | Issue 1/2015

Abstract

Herein, we describe the establishment and characterization of the first mixed-phenotype acute leukemia cell line (JIH-5). The JIH-5 cell line was established from leukemia cells with B lymphoid/myeloid phenotype from a female mixed-phenotype acute leukemia patient. JIH-5 cells exhibit an immunophenotype comprised of myeloid and B lymphoid antigens. Whole-exome sequencing revealed somatic mutations in nine genes in JIH-5 cells. Transcriptional sequencing of JIH-5 cells identified EP300-ZNF384 fusion transcript, which is a recurrent alteration in B cell acute lymphoblastic leukemia. Our results suggest that the JIH-5 cell line may serve as a tool for the study of mixed-phenotype acute leukemia or EP300-ZNF384.

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Title: Establishment and genetic characterization of a novel mixed-phenotype acute leukemia cell line with EP300-ZNF384 fusion
Authors: Nana Ping
Huiying Qiu
Qian Wang
Haiping Dai
Changgeng Ruan
Stefan Ehrentraut
Hans G. Drexler
Roderick A. F. MacLeod
Suning Chen
Publication date: 01-12-2015
Publisher: BioMed Central
Published in: Journal of Hematology & Oncology / Issue 1/2015
Electronic ISSN: 1756-8722
DOI: https://doi.org/10.1186/s13045-015-0197-2

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