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Journal of Orthopaedic Surgery and Research

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Open Access 01-12-2018 | Research article

Mechanical barriers and transforming growth factor beta inhibitor on epidural fibrosis in a rabbit laminectomy model

Authors: Juan N. Albiñana-Cunningham, Purificación Ripalda-Cemboráin, Tania Labiano, José I. Echeveste, Froilán Granero-Moltó, Matías Alfonso-Olmos

Published in: Journal of Orthopaedic Surgery and Research | Issue 1/2018

Abstract

Background

TGF-β has been described as a mediator of fibrosis and scarring. Several studies achieved reduction in experimental scarring through the inhibition of TGF-β. Fibroblasts have been defined as the cell population originating fibrosis, blocking fibroblast invasion may impair epidural fibrosis appearance. For this purpose, biocompatible materials used as mechanical barriers and a TGF-β inhibitor peptide were evaluated in the reduction of epidural fibrosis.

Methods

A L6 laminectomy was performed in 40 New Zealand white rabbits. Divided into four groups, each rabbit was assigned to receive either collagen sponge scaffold (CS group), gelatin-based gel (GCP group), P144® (iTGFβ group), or left untreated (control group). Four weeks after surgery, cell density, collagen content, and new bone formation of the scar area were determined by histomorphometry. Two experienced pathologists scored dura mater adhesion, scar density, and inflammatory infiltrate in a blinded manner.

Results

In all groups, laminectomy site was filled with fibrous tissue and the dura mater presented adhesions. Only GCP group presented a significant reduction in collagen content and scar density.

Conclusion

GCP treatment reduces epidural fibrosis although did not prevent dura mater adhesion completely.

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Title: Mechanical barriers and transforming growth factor beta inhibitor on epidural fibrosis in a rabbit laminectomy model
Authors: Juan N. Albiñana-Cunningham
Purificación Ripalda-Cemboráin
Tania Labiano
José I. Echeveste
Froilán Granero-Moltó
Matías Alfonso-Olmos
Publication date: 01-12-2018
Publisher: BioMed Central
Published in: Journal of Orthopaedic Surgery and Research / Issue 1/2018
Electronic ISSN: 1749-799X
DOI: https://doi.org/10.1186/s13018-018-0781-6

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Springer Medicine

Mechanical barriers and transforming growth factor beta inhibitor on epidural fibrosis in a rabbit laminectomy model

Abstract

Background

Methods

Results

Conclusion

Keynote webinar | Spotlight on sleep in brain health

Springer Medicine

Abstract

Background

Methods

Results

Conclusion

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