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Journal of Orthopaedic Surgery and Research
Published in: Journal of Orthopaedic Surgery and Research 1/2016

Open Access 01-12-2016 | Research article

The effect of erythropoietin on biomechanical properties of the Achilles tendon during the healing process: an experimental study

Authors: Okkes Bilal, Ahmet Guney, Ali Murat Kalender, Ibrahim Halil Kafadar, Muzaffer Yildirim, Nuh Dundar

Published in: Journal of Orthopaedic Surgery and Research | Issue 1/2016

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Abstract

Background

The aim of this study was to examine the potential biomechanical and histological benefits of systemic erythropoietin administration during the healing of Achilles tendon injury in a rat experimental model.

Methods

Eighty Sprague-Dawley female rats were included in this study. Animals were randomly assigned into two groups with 40 animals in each: erythropoietin group and control group. Then each group was further divided into four subgroups corresponding to four time points with 10 animals in each. A full-thickness cut was made on the Achilles tendon of each animal and then the tendon was sutured with modified Kessler method. Erythropoietin groups received intraperitoneal erythropoietin (500 IU/kg/day) every day at same time throughout the study period, and the control groups received saline in a similar manner. Animals were sacrificed at four time points, and tensile test was performed on each tendon sample to assess maximum load for each sample. In addition, histopathological examination and scoring was done.

Results

Both groups had improvement on tensile test (maximum load) over time. However, groups did not differ with regard to maximum load in any of the time points. Similarly, groups did not differ with regard to any of the histopathological scores over time.

Conclusions

The findings of this study do not support the benefit of systemic erythropoietin administration in Achilles tendon healing process. Further evidence from larger experimental studies is required to justify any such potential benefit.
Appendix
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Metadata
Title
The effect of erythropoietin on biomechanical properties of the Achilles tendon during the healing process: an experimental study
Authors
Okkes Bilal
Ahmet Guney
Ali Murat Kalender
Ibrahim Halil Kafadar
Muzaffer Yildirim
Nuh Dundar
Publication date
01-12-2016
Publisher
BioMed Central
Published in
Journal of Orthopaedic Surgery and Research / Issue 1/2016
Electronic ISSN: 1749-799X
DOI
https://doi.org/10.1186/s13018-016-0390-1

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