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Open Access 01-12-2018 | Research

PD1^hi cells associate with clusters of proliferating B-cells in marginal zone lymphoma

Authors: Katherine Wickenden, Nadia Nawaz, Sami Mamand, Deevia Kotecha, Amy L. Wilson, Simon D. Wagner, Matthew J. Ahearne

Published in: Diagnostic Pathology | Issue 1/2018

Abstract

Background

Abnormally sustained immune reactions drive B-cell proliferation in some cases of marginal zone lymphoma but the CD4⁺ T-cell subsets, which are likely to contribute to the B-cell responses in the tumour microenvironment, are not well characterised and neither has the spatial distribution of the different subsets in involved lymph nodes been investigated.

Methods

Employing a workflow of multiplex semi-automated immunohistochemistry combined with image processing we investigated association between infiltrating T-cells and proliferating lymphoma B-cells.

Results

Both total numbers of activating follicular helper (Tfh) cells (defined by high expression of PD1) and suppressive regulatory (Treg) T-cells (defined by FOXP3⁺ expression) and the Tfh:Treg ratio, assessed over relatively large areas of tissue, varied among cases of marginal zone lymphoma. We determined spatial distribution and demonstrated that PD1^hi cells showed significantly more clustering than did FOXP3⁺. To investigate the association of infiltrating T-cells with lymphoma B-cells we employed Pearson correlation and Morisita-Horn index, statistical measures of interaction. We demonstrated that PD1^hi cells were associated with proliferating B-cells and confirmed this by nearest neighbour analysis.

Conclusions

The unexpected architectural complexity of T-cell infiltration in marginal zone lymphoma, revealed in this study, further supports a key role for Tfh cells in driving proliferation of lymphoma B-cells. We demonstrate the feasibility of digital analysis of spatial architecture of T-cells within marginal zone lymphoma and future studies will be needed to determine the clinical importance of these observations.

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Title: PD1hi cells associate with clusters of proliferating B-cells in marginal zone lymphoma
Authors: Katherine Wickenden
Nadia Nawaz
Sami Mamand
Deevia Kotecha
Amy L. Wilson
Simon D. Wagner
Matthew J. Ahearne
Publication date: 01-12-2018
Publisher: BioMed Central
Published in: Diagnostic Pathology / Issue 1/2018
Electronic ISSN: 1746-1596
DOI: https://doi.org/10.1186/s13000-018-0750-8

Keynote webinar | Spotlight on sleep in brain health

Springer Medicine

PD1^hi cells associate with clusters of proliferating B-cells in marginal zone lymphoma

Abstract

Background

Methods

Results

Conclusions

Keynote webinar | Spotlight on sleep in brain health

Springer Medicine

Abstract

Background

Methods

Results

Conclusions

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