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Virology Journal
Published in: Virology Journal 1/2019

Open Access 01-12-2019 | Research

Antiviral activity of Piscidin 1 against pseudorabies virus both in vitro and in vivo

Authors: Han Hu, Nan Guo, Shuhua Chen, Xiaozhen Guo, Xiaoli Liu, Shiyi Ye, Qingqing Chai, Yang Wang, Binlei Liu, Qigai He

Published in: Virology Journal | Issue 1/2019

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Abstract

Background

Swine-origin virus infection spreading widely could cause significant economic loss to porcine industry. Novel antiviral agents need to be developed to control this situation.

Methods

In this study, we evaluated the activities of five broad-spectrum antimicrobial peptides (AMPs) against several important swine-origin pathogenic viruses by TCID50 assay. Plaque reduction assay and cell apoptosis assay were also used to test the activity of the peptides. Protection effect of piscidin against pseudorabies virus (PRV) was also examined in mouse model.

Results

Piscidin (piscidin 1), caerin (caerin 1.1) and maculatin (maculatin 1.1) could inhibit PRV by direct interaction with the virus particles in a dose-dependent manner and they could also protect the cells from PRV-induced apoptosis. Among the peptides tested, piscidin showed the strongest activity against PRV. Moreover, in vivo assay showed that piscidin can reduce the mortality of mice infected with PRV.

Conclusion

In vitro and in vivo experiments indicate that piscidin has antiviral activity against PRV.
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Metadata
Title
Antiviral activity of Piscidin 1 against pseudorabies virus both in vitro and in vivo
Authors
Han Hu
Nan Guo
Shuhua Chen
Xiaozhen Guo
Xiaoli Liu
Shiyi Ye
Qingqing Chai
Yang Wang
Binlei Liu
Qigai He
Publication date
01-12-2019
Publisher
BioMed Central
Published in
Virology Journal / Issue 1/2019
Electronic ISSN: 1743-422X
DOI
https://doi.org/10.1186/s12985-019-1199-4

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