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Open Access 01-12-2019 | Pseudomonas | Research

Complete genome sequences of Aeromonas and Pseudomonas phages as a supportive tool for development of antibacterial treatment in aquaculture

Authors: Joanna Kazimierczak, Ewelina Agnieszka Wójcik, Jolanta Witaszewska, Arkadiusz Guziński, Elżbieta Górecka, Małgorzata Stańczyk, Edyta Kaczorek, Andrzej Krzysztof Siwicki, Jarosław Dastych

Published in: Virology Journal | Issue 1/2019

Abstract

Background

Aquaculture is the fastest growing sector of food production worldwide. However, one of the major reasons limiting its effectiveness are infectious diseases among aquatic organisms resulting in vast economic losses. Fighting such infections with chemotherapy is normally used as a rapid and effective treatment. The rise of antibiotic resistance, however, is limiting the efficacy of antibiotics and creates environmental and human safety concerns due to their massive application in the aquatic environment. Bacteriophages are an alternative solution that could be considered in order to protect fish against pathogens while minimizing the side-effects for the environment and humans. Bacteriophages kill bacteria via different mechanisms than antibiotics, and so fit nicely into the ‘novel mode of action’ concept desired for all new antibacterial agents.

Methods

The bacteriophages were isolated from sewage water and characterized by RFLP, spectrum of specificity, transmission electron microscopy (TEM) and sequencing (WGS). Bioinformatics analysis of genomic data enables an in-depth characterization of phages and the choice of phages. This allows an optimised choice of phage for therapy, excluding those with toxin genes, virulence factor genes, and genes responsible for lysogeny.

Results

In this study, we isolated eleven new bacteriophages: seven infecting Aeromonas and four infecting Pseudomonas, which significantly increases the genomic information of Aeromonas and Pseudomonas phages. Bioinformatics analysis of genomic data, assessing the likelihood of these phages to enter the lysogenic cycle with experimental data on their specificity towards large number of bacterial field isolates representing different locations.

Conclusions

From 11 newly isolated bacteriophages only 6 (25AhydR2PP, 50AhydR13PP, 60AhydR15PP, 22PfluR64PP, 67PfluR64PP, 71PfluR64PP) have a potential to be used in phage therapy due to confirmed lytic lifestyle and absence of virulence or resistance genes.

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Title: Complete genome sequences of Aeromonas and Pseudomonas phages as a supportive tool for development of antibacterial treatment in aquaculture
Authors: Joanna Kazimierczak
Ewelina Agnieszka Wójcik
Jolanta Witaszewska
Arkadiusz Guziński
Elżbieta Górecka
Małgorzata Stańczyk
Edyta Kaczorek
Andrzej Krzysztof Siwicki
Jarosław Dastych
Publication date: 01-12-2019
Publisher: BioMed Central
Keyword: Pseudomonas
Published in: Virology Journal / Issue 1/2019
Electronic ISSN: 1743-422X
DOI: https://doi.org/10.1186/s12985-018-1113-5

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Complete genome sequences of Aeromonas and Pseudomonas phages as a supportive tool for development of antibacterial treatment in aquaculture

Abstract

Background

Methods

Results

Conclusions

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Abstract

Background

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Results

Conclusions

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