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Open Access 01-12-2019 | Polymerase Chain Reaction | Short report

Development of a duplex SYBR Green I-based quantitative real-time PCR assay for the rapid differentiation of goose and Muscovy duck parvoviruses

Authors: Su Lin, Shao Wang, Xiaoxia Cheng, Shifeng Xiao, Xiuqin Chen, Shilong Chen, Shaoying Chen, Fusong Yu

Published in: Virology Journal | Issue 1/2019

Abstract

Background

Waterfowl parvoviruses, including goose parvovirus (GPV) and Muscovy duck parvovirus (MDPV), can cause seriously diseases in geese and ducks. Developing a fast and precise diagnosis assay for these two parvoviruses is particularly important.

Results

A duplex SYBR Green I-based quantitative real-time PCR assay was developed for the simultaneous detection and differentiation of GPV and MDPV. The assay yielded melting curves with specific single peak (Tm = 87.3 ± 0.26 °C or Tm = 85.4 ± 0.23 °C) when GPV or MDPV was evaluated, respectively. When both parvoviruses were assessed in one reaction, melting curves with specific double peaks were yielded.

Conclusion

This duplex quantitative RT-PCR can be used to rapid identify of GPV and MDPV in field cases and artificial trials, which make it a powerful tool for diagnosing, preventing and controlling waterfowl parvovirus infections.

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Title: Development of a duplex SYBR Green I-based quantitative real-time PCR assay for the rapid differentiation of goose and Muscovy duck parvoviruses
Authors: Su Lin
Shao Wang
Xiaoxia Cheng
Shifeng Xiao
Xiuqin Chen
Shilong Chen
Shaoying Chen
Fusong Yu
Publication date: 01-12-2019
Publisher: BioMed Central
Keyword: Polymerase Chain Reaction
Published in: Virology Journal / Issue 1/2019
Electronic ISSN: 1743-422X
DOI: https://doi.org/10.1186/s12985-018-1111-7

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Development of a duplex SYBR Green I-based quantitative real-time PCR assay for the rapid differentiation of goose and Muscovy duck parvoviruses

Abstract

Background

Results

Conclusion

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Abstract

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Conclusion

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