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Virology Journal

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Open Access 01-12-2018 | Research

Ubiquitin-conjugating enzyme UBE2J1 negatively modulates interferon pathway and promotes RNA virus infection

Authors: Tingting Feng, Lei Deng, Xiaochuan Lu, Wen Pan, Qihan Wu, Jianfeng Dai

Published in: Virology Journal | Issue 1/2018

Abstract

Background

Viral infection activates innate immune pathways and interferons (IFNs) play a pivotal role in the outcome of a viral infection. Ubiquitin modifications of host and viral proteins significantly influence the progress of virus infection. Ubiquitin-conjugating enzyme E2s (UBE2) have the capacity to determine ubiquitin chain topology and emerge as key mediators of chain assembly.

Methods

In this study, we screened the functions of 34 E2 genes using an RNAi library during Dengue virus (DENV) infection. RNAi and gene overexpression approaches were used to study the gene function in viral infection and interferon signaling.

Results

We found that silencing UBE2J1 significantly impaired DENV infection, while overexpression of UBE2J1 enhanced DENV infection. Further studies suggested that type I IFN expression was significantly increased in UBE2J1 silenced cells and decreased in UBE2J1 overexpressed cells. Reporter assay suggested that overexpression of UBE2J1 dramatically suppressed RIG-I directed IFNβ promoter activation. Finally, we have confirmed that UBE2J1 can facilitate the ubiquitination and degradation of transcription factor IFN regulatory factor 3 (IRF3).

Conclusion

These results suggest that UBE2 family member UBE2J1 can negatively regulate type I IFN expression, thereby promote RNA virus infection.

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Title: Ubiquitin-conjugating enzyme UBE2J1 negatively modulates interferon pathway and promotes RNA virus infection
Authors: Tingting Feng
Lei Deng
Xiaochuan Lu
Wen Pan
Qihan Wu
Jianfeng Dai
Publication date: 01-12-2018
Publisher: BioMed Central
Published in: Virology Journal / Issue 1/2018
Electronic ISSN: 1743-422X
DOI: https://doi.org/10.1186/s12985-018-1040-5

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Ubiquitin-conjugating enzyme UBE2J1 negatively modulates interferon pathway and promotes RNA virus infection

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Background

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Results

Conclusion

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