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Open Access 01-12-2017 | Research

ORF1a of highly pathogenic PRRS attenuated vaccine virus plays a key role in neutralizing antibody induction in piglets and virus neutralization in vitro

Authors: Chaoliang Leng, Wuchao Zhang, Hongliang Zhang, Yunchao Kan, Lunguang Yao, Hongyue Zhai, Mingliang Li, Zhen Li, Chunxiao Liu, Tongqing An, Jinmei Peng, Qian Wang, Yumin Leng, Xuehui Cai, Zhijun Tian, Guangzhi Tong

Published in: Virology Journal | Issue 1/2017

Abstract

Background

Currently, porcine reproductive and respiratory syndrome virus (PRRSV) is one of the most economically important viral pathogens in swine in most countries, especially China. Two PRRSV attenuated live vaccine strains (HuN4-F112 and CH-1R) are currently widely used in China. Our previous study showed that HuN4-F112, but not CH-1R, induced high anti-nucleocapsid (N) antibody and neutralizing antibody (NA) titers. Additionally, sera from HuN4-F112 inoculated pigs induced low cross neutralization of CH-1R.

Methods

In the present study, 6 chimeric viruses through exchanging 5′ untranslated region (UTR) + open reading frame (ORF)1a, ORF1b, and ORF2–7 + 3’UTR between HuN4-F112 and CH-1R were constructed and rescued based on the infectious clones of rHuN4-F112 and rCH-1R. The characteristics of these viruses were investigated in vitro and vivo.

Results

All the three fragments, 5’UTR + ORF1a, ORF1b, and ORF2–7 + 3’UTR, could affect the replication efficiencies of rHuN4-F112 and rCH-1R in vitro. Additionally, both 5’UTR + ORF1a and ORF2–7 + 3’UTR affected the anti-N antibody and NA responses targeting rHuN4-F112 and rCH-1R in piglets.

Conclusions

The 5’UTR + ORF1a region of HuN4-F112 played a key role in inducing NAs in piglets. Furthermore, we confirmed for the first time that ORF1a contains a neutralization region. This study provides important information that can be used for further study of the generation of anti-PRRSV NAs.

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Title: ORF1a of highly pathogenic PRRS attenuated vaccine virus plays a key role in neutralizing antibody induction in piglets and virus neutralization in vitro
Authors: Chaoliang Leng
Wuchao Zhang
Hongliang Zhang
Yunchao Kan
Lunguang Yao
Hongyue Zhai
Mingliang Li
Zhen Li
Chunxiao Liu
Tongqing An
Jinmei Peng
Qian Wang
Yumin Leng
Xuehui Cai
Zhijun Tian
Guangzhi Tong
Publication date: 01-12-2017
Publisher: BioMed Central
Published in: Virology Journal / Issue 1/2017
Electronic ISSN: 1743-422X
DOI: https://doi.org/10.1186/s12985-017-0825-2

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ORF1a of highly pathogenic PRRS attenuated vaccine virus plays a key role in neutralizing antibody induction in piglets and virus neutralization in vitro

Abstract

Background

Methods

Results

Conclusions

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Abstract

Background

Methods

Results

Conclusions

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