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Open Access 01-12-2017 | Research

Transcription profiles of the responses of chicken bursae of Fabricius to IBDV in different timing phases

Authors: Changbo Ou, Qiuxia Wang, Yanhong Zhang, Weili Kong, Shouping Zhang, Yan Yu, Jinyou Ma, Xingyou Liu, Xianghui Kong

Published in: Virology Journal | Issue 1/2017

Abstract

Background

Infectious bursal disease virus (IBDV) infection causes immunosuppression in chickens and increases their susceptibility to secondary infections. To explore the interaction between host and IBDV, RNA-Seq was applied to analyse the transcriptional profiles of the responses of chickens’ bursas of Fabricius in the early stage of IBDV infection.

Results

The results displayed that a total of 15546 genes were identified in the chicken bursa libraries. Among the annotated genes, there were 2006 and 4668 differentially expressed genes in the infection group compared with the mock group on day 1 and day 3 post inoculation (1 and 3 dpi), respectively. Moreover, there were 676 common up-regulated and 83 common down-regulated genes in the bursae taken from the chickens infected with IBDV on both 1 and 3 dpi. Meanwhile, there were also some characteristic differentially expressed genes on 1 and 3 dpi. On day 1 after inoculation with IBDV, host responses mainly displayed immune response processes, while metabolic pathways played an important role on day three post infection. Six genes were confirmed by quantitative reverse transcription-PCR.

Conclusions

In conclusion, the differential gene expression profile demonstrated with RNA-Seq might offer a better understanding of the molecular interactions between host and IBDV during the early stage of infection.

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Title: Transcription profiles of the responses of chicken bursae of Fabricius to IBDV in different timing phases
Authors: Changbo Ou
Qiuxia Wang
Yanhong Zhang
Weili Kong
Shouping Zhang
Yan Yu
Jinyou Ma
Xingyou Liu
Xianghui Kong
Publication date: 01-12-2017
Publisher: BioMed Central
Published in: Virology Journal / Issue 1/2017
Electronic ISSN: 1743-422X
DOI: https://doi.org/10.1186/s12985-017-0757-x

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