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Open Access 01-12-2017 | Short report

Transcriptional profiling of host cell responses to encephalomyocarditis virus (EMCV)

Authors: Jia Wei, Haixia Zhang, Xiangrong Li, Qiongyi Li, Zhongren Ma, Jialin Bai, Zilin Qiao, Ruofei Feng

Published in: Virology Journal | Issue 1/2017

Abstract

Backgroud

Encephalomyocarditis virus (EMCV) has been discovered on pig farms worldwide and can cause myocarditis in piglets and reproductive failure in sows. However, little is known about the host transcriptional responses to infection and host-pathogen interactions.

Methods

In this study, transcription profiling was performed by Illumina RNA-Sequencing (RNA-seq) to identify EMCV induced differentially expressed genes in BHK-21 cells at serial time points (12, 24, and 30 h post infection (hpi)), using mock infected cells as control.

Results

We identified 237, 241, and 207 differentially expressed genes (DEGs) respectively, majority of which were up-regulated. A large number of DEGs clustered into host defense, cellular signaling and metabolism categories. Moreover, short time series expression analysis revealed that 12 hpi was an important time point for expression change, indicating host virus resistance.

Conclusions

This RNA-seq analysis provides the first data for understanding the network of virus host interactions under EMCV infection in vitro, and for identifying host components which involved in the virus infection course.

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Title: Transcriptional profiling of host cell responses to encephalomyocarditis virus (EMCV)
Authors: Jia Wei
Haixia Zhang
Xiangrong Li
Qiongyi Li
Zhongren Ma
Jialin Bai
Zilin Qiao
Ruofei Feng
Publication date: 01-12-2017
Publisher: BioMed Central
Published in: Virology Journal / Issue 1/2017
Electronic ISSN: 1743-422X
DOI: https://doi.org/10.1186/s12985-017-0718-4

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Transcriptional profiling of host cell responses to encephalomyocarditis virus (EMCV)

Abstract

Backgroud

Methods

Results

Conclusions

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Abstract

Backgroud

Methods

Results

Conclusions

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