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Open Access 01-12-2022 | Chronic Kidney Disease | Research

Anthocyanin improves kidney function in diabetic kidney disease by regulating amino acid metabolism

Authors: Yi-Xi Li, Yong-Ping Lu, Donge Tang, Bo Hu, Ze-Yu Zhang, Hong-Wei Wu, Li-Jing Fan, Kai-Wen Cai, Chun Tang, Yi-Qing Zhang, Ling Hong, Jing-jing Dong, Bao-zhang Guan, Liang-Hong Yin, Yong Dai, Wei-bin Bai, Zhi-Hua Zheng, Ting Zhu

Published in: Journal of Translational Medicine | Issue 1/2022

Abstract

Background

Diabetic kidney disease (DKD) is among the most important causes for chronic kidney disease. Anthocyanins (ANT) are polyphenolic compounds present in various food and play an important role in ameliorating hyperglycemia and insulin sensitivity. However, the effects of ANT in DKD are still poorly understood. This study aimed to investigate the effect of ANT (cyanidin-3-O-glucoside [C3G]) on the renal function of DKD, and whether the anti-DKD effect of ANT is related to metabolic pathways.

Methods

To explore the role of ANT in DKD, we performed the examination of blood glucose, renal function, and histopathology. As for the mechanism, we designed the label-free quantification proteomics and nontargeted metabolomics analysis for kidney and serum. Subsequently, we revealed the anti-DKD effect of ANT through the bioinformatic analysis.

Results

We showed that the fasting blood glucose level (− 6.1 mmol/L, P = 0.037), perimeter of glomerular lesions (− 24.1 μm, P = 0.030), fibrosis score of glomerular (− 8.8%, P = 0.002), and kidney function (Cystatin C: − 701.4 pg/mL, P = 0.043; urine creatinine: − 701.4 mmol/L, P = 0.032) were significantly alleviated in DKD mice after ANT treatment compared to untreated in the 20th week. Further, proteins and metabolites in the kidneys of DKD mice were observed to be dramatically altered due to changes in amino acid metabolism with ANT treatment; mainly, taurine and hypotaurine metabolism pathway was upregulated (P = 0.0001, t value = 5.97). Furthermore, upregulated tryptophan metabolism (P < 0.0001, t value = 5.94) and tyrosine metabolism (P = 0.0037, t value = 2.91) pathways had effects on serum of DKD mice as responsed ANT regulating.

Conclusions

Our results suggested that prevention of the progression of DKD by ANT could be related to the regulation of amino acid metabolism. The use of dietary ANT may be one of the dietary strategies to prevent and treat DKD.

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Title: Anthocyanin improves kidney function in diabetic kidney disease by regulating amino acid metabolism
Authors: Yi-Xi Li
Yong-Ping Lu
Donge Tang
Bo Hu
Ze-Yu Zhang
Hong-Wei Wu
Li-Jing Fan
Kai-Wen Cai
Chun Tang
Yi-Qing Zhang
Ling Hong
Jing-jing Dong
Bao-zhang Guan
Liang-Hong Yin
Yong Dai
Wei-bin Bai
Zhi-Hua Zheng
Ting Zhu
Publication date: 01-12-2022
Publisher: BioMed Central
Keyword: Chronic Kidney Disease
Published in: Journal of Translational Medicine / Issue 1/2022
Electronic ISSN: 1479-5876
DOI: https://doi.org/10.1186/s12967-022-03717-9

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