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Journal of Translational Medicine
Published in: Journal of Translational Medicine 1/2022

Open Access 01-12-2022 | Myocardial Infarction | Research

Heart-specific DNA methylation analysis in plasma for the investigation of myocardial damage

Authors: Jie Ren, Lin Jiang, Xiaomeng Liu, Yuhan Liao, Xueyan Zhao, Fuchou Tang, Huimin Yu, Yibing Shao, Jizheng Wang, Lu Wen, Lei Song

Published in: Journal of Translational Medicine | Issue 1/2022

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Abstract

Background

Circulating cell-free DNA (cfDNA) can be released when myocardial damage occurs.

Methods

Here, we used the methylated CpG tandem amplification and sequencing (MCTA-seq) method for analyzing dynamic changes in heart-derived DNA in plasma samples from myocardial infarction (MI) patients.

Results

We identified six CGCGCGG loci showing heart-specific hypermethylation patterns. MCTA-seq deconvolution analysis combining these loci detected heart-released cfDNA in MI patients at hospital admission, and showed that the prominently elevated total cfDNA level after percutaneous coronary intervention (PCI) was derived from both the heart and white blood cells. Furthermore, for the top marker CORO6, we developed a digital droplet PCR (ddPCR) assay that clearly detected heart damage signals in cfDNA of MI patients at hospital admission.

Conclusions

Our study provides insights into MI pathologies and developed a new ddPCR assay for detecting myocardial damage in clinical applications.
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Metadata
Title
Heart-specific DNA methylation analysis in plasma for the investigation of myocardial damage
Authors
Jie Ren
Lin Jiang
Xiaomeng Liu
Yuhan Liao
Xueyan Zhao
Fuchou Tang
Huimin Yu
Yibing Shao
Jizheng Wang
Lu Wen
Lei Song
Publication date
01-12-2022
Publisher
BioMed Central
Published in
Journal of Translational Medicine / Issue 1/2022
Electronic ISSN: 1479-5876
DOI
https://doi.org/10.1186/s12967-022-03234-9

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