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Open Access 01-12-2020 | Research

Regulation of [Ca²⁺]_i oscillations and mitochondrial activity by various calcium transporters in mouse oocytes

Authors: Feng Wang, Ang Li, Tie-Gang Meng, Le-Yun Wang, Li-Juan Wang, Yi Hou, Heide Schatten, Qing-Yuan Sun, Xiang-Hong Ou

Published in: Reproductive Biology and Endocrinology | Issue 1/2020

Abstract

Oocyte activation inefficiency is one of the reasons for female infertility and Ca²⁺ functions play a critical role in the regulation of oocyte activation. We used various inhibitors of Ca²⁺ channels located on the membrane, including sarcoplasmic/ endoplasmic reticulum Ca²⁺ATPases (SERCAs, the main Ca²⁺ pumps which decrease the intracellular Ca²⁺ level by refilling Ca²⁺ into the sarcoplasmic reticulum), transient receptor potential (TRP) ion channel subfamily member 7 (TRPM7, a Ca²⁺/Mg²⁺-permeable non-selective cation channel), T-type Ca²⁺ channels and calcium channel Orai1, to investigate their roles in [Ca²⁺]_i oscillation patterns and mitochondrial membrane potential during oocyte activation by real-time recording. Our results showed that SERCAs, TRPM7 and T-type Ca²⁺ channels were important for initiation and maintenance of [Ca²⁺]_i oscillations, which was required for mitochondrial membrane potential elevation during oocyte activation, as well as oocyte cytoskeleton stability and subsequent embryo development. Increasing the knowledge of calcium transport may provide a theoretical basis for improving oocyte activation in human assisted reproduction clinics.

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Title: Regulation of [Ca2+]i oscillations and mitochondrial activity by various calcium transporters in mouse oocytes
Authors: Feng Wang
Ang Li
Tie-Gang Meng
Le-Yun Wang
Li-Juan Wang
Yi Hou
Heide Schatten
Qing-Yuan Sun
Xiang-Hong Ou
Publication date: 01-12-2020
Publisher: BioMed Central
Published in: Reproductive Biology and Endocrinology / Issue 1/2020
Electronic ISSN: 1477-7827
DOI: https://doi.org/10.1186/s12958-020-00643-7

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Regulation of [Ca²⁺]_i oscillations and mitochondrial activity by various calcium transporters in mouse oocytes

Abstract

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Abstract

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