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Reproductive Biology and Endocrinology
Published in: Reproductive Biology and Endocrinology 1/2017

Open Access 01-12-2017 | Research

The decreased expression of TIPE2 protein in the decidua of patients with missed abortion and possible significance

Authors: Yingshuo Sun, Xiaoyan Wang, Yue Li, Han Sun, Lu Wan, Xishuang Wang, Lining Zhang, Zhenghui Fang, Zengtao Wei

Published in: Reproductive Biology and Endocrinology | Issue 1/2017

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Abstract

Background

Missed abortion is a common occurrence for otherwise healthy women. Immunological factor is one of the most important reasons. Tumor necrosis factor-α-induced protein-8 like-2 (TIPE2) is a novel negative immune regulator related to several human diseases. However, the expression level and clinical significance of TIPE2 in missed abortion remain unclear.

Methods

The expression of TIPE2 mRNA and protein in decidua and chorion from 36 missed abortion patients and 36 healthy controls was detected using quantitative real-time PCR, western blot and immunohistochemistry. In addition, serum TNF-ɑ and IL-10 levels were measured using flow cytometry. Serum estradiol and progesterone levels were measured by radioimmunoassay test. The correlations of TIPE2 protein levels with TNF-ɑ, IL-10, estradiol and progesterone were further analyzed.

Results

TIPE2 protein levels were significantly lower in decidual tissues of missed abortion patients than those in healthy controls. The patients with missed abortion had significantly higher levels of serum TNF-ɑ, and lower levels of serum IL-10, estradiol and progesterone compared with healthy controls. The TIPE2 protein levels were positively related to serum IL-10 levels.

Conclusion

Our data indicate TIPE2 could play important roles in maintaining the maternal-fetal tolerance and decreased TIPE2 expression in the decidua may be related to the development of missed abortion.
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Metadata
Title
The decreased expression of TIPE2 protein in the decidua of patients with missed abortion and possible significance
Authors
Yingshuo Sun
Xiaoyan Wang
Yue Li
Han Sun
Lu Wan
Xishuang Wang
Lining Zhang
Zhenghui Fang
Zengtao Wei
Publication date
01-12-2017
Publisher
BioMed Central
Published in
Reproductive Biology and Endocrinology / Issue 1/2017
Electronic ISSN: 1477-7827
DOI
https://doi.org/10.1186/s12958-017-0285-y

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