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Malaria Journal
Published in: Malaria Journal 1/2020

01-12-2020 | Plasmodium Falciparum | Research

Identification of Plasmodium falciparum proteoforms from liver stage models

Authors: Benjamin Winer, Kimberly A. Edgel, Xiaoyan Zou, Julie Sellau, Sri Hadiwidjojo, Lindsey S. Garver, Christin E. McDonough, Neil L. Kelleher, Paul M. Thomas, Eileen Villasante, Alexander Ploss, Vincent R. Gerbasi

Published in: Malaria Journal | Issue 1/2020

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Abstract

Background

Immunization with attenuated malaria sporozoites protects humans from experimental malaria challenge by mosquito bite. Protection in humans is strongly correlated with the production of T cells targeting a heterogeneous population of pre-erythrocyte antigen proteoforms, including liver stage antigens. Currently, few T cell epitopes derived from Plasmodium falciparum, the major aetiologic agent of malaria in humans are known.

Methods

In this study both in vitro and in vivo malaria liver stage models were used to sequence host and pathogen proteoforms. Proteoforms from these diverse models were subjected to mild acid elution (of soluble forms), multi-dimensional fractionation, tandem mass spectrometry, and top-down bioinformatics analysis to identify proteoforms in their intact state.

Results

These results identify a group of host and malaria liver stage proteoforms that meet a 5% false discovery rate threshold.

Conclusions

This work provides proof-of-concept for the validity of this mass spectrometry/bioinformatic approach for future studies seeking to reveal malaria liver stage antigens towards vaccine development.
Appendix
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Metadata
Title
Identification of Plasmodium falciparum proteoforms from liver stage models
Authors
Benjamin Winer
Kimberly A. Edgel
Xiaoyan Zou
Julie Sellau
Sri Hadiwidjojo
Lindsey S. Garver
Christin E. McDonough
Neil L. Kelleher
Paul M. Thomas
Eileen Villasante
Alexander Ploss
Vincent R. Gerbasi
Publication date
01-12-2020
Publisher
BioMed Central
Published in
Malaria Journal / Issue 1/2020
Electronic ISSN: 1475-2875
DOI
https://doi.org/10.1186/s12936-019-3093-3

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