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Published in: Malaria Journal 1/2018

Open Access 01-12-2018 | Research

External quality assessment of malaria microscopy diagnosis in selected health facilities in Western Oromia, Ethiopia

Authors: Getachew Sori, Olifan Zewdie, Geletta Tadele, Abdi Samuel

Published in: Malaria Journal | Issue 1/2018

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Abstract

Background

Accurate early diagnosis and prompt treatment are one of the key strategies to control and prevent malaria disease. External quality assessment is the most effective method for evaluation of the quality of malaria microscopy diagnosis. The aim of this study was to assess the quality of malaria microscopy diagnosis and its associated factors in selected public health facility laboratories in East Wollega Zone, Western Ethiopia.

Methods

Facility-based cross-sectional study design was conducted in 30 randomly selected public health facility laboratories from November 2014 to January 2015 in East Wollega Zone, Western Ethiopia. Ten validated stained malaria panel slides with known Plasmodium species, developmental stage and parasite density were distributed. Data were captured; cleaned and analyzed using SPSS version 20 statistical software-multivariate logistic regressions and the agreement in reading between the peripheral diagnostic centers and the reference laboratory were done using kappa statistics.

Results

A total of 30 health facility laboratories were involved in the study and the overall quality of malaria microscopy diagnosis was poor (62.3%). The associated predictors of quality in this diagnosis were in-service training [(AOR = 16, 95% CI (1.3, 1.96)], smearing quality [(AOR = 24, 95% CI (1.8, 3.13)], staining quality [(AOR = 15, 95% CI (2.35, 8.61), parasite detection [(AOR = 9, 95% CI (1.1, 8.52)] and identification skills [(AOR = 8.6, 95% CI (1.21, 1.63)]. Eighteen (60%) of health facility laboratories had in-service trained laboratory professionals on malaria microscopy diagnosis.

Conclusion

Overall quality of malaria microscopy diagnosis was poor and a significant gap in this service was observed that could impact on its diagnostic services.
Literature
1.
go back to reference WHO. World malaria report. Geneva: World Health Organization; 2017. WHO. World malaria report. Geneva: World Health Organization; 2017.
2.
go back to reference Cheesbrough M. District laboratory practice in tropical countries. Cambridge: Cambridge University Press; 2006. p. 454.CrossRef Cheesbrough M. District laboratory practice in tropical countries. Cambridge: Cambridge University Press; 2006. p. 454.CrossRef
3.
go back to reference Raghuveer C, Rajeev A, Bhandari P. Comparative study of peripheral blood smear, quantitative buffy coat and modified centrifuged blood smear in malaria diagnosis. Indian J Pathol Microbiol. 2008;51:108–12.CrossRefPubMed Raghuveer C, Rajeev A, Bhandari P. Comparative study of peripheral blood smear, quantitative buffy coat and modified centrifuged blood smear in malaria diagnosis. Indian J Pathol Microbiol. 2008;51:108–12.CrossRefPubMed
4.
go back to reference Kettelhut M, Chiodini P, Edwards H, Moody A. External quality assessment schemes raise standards: evidence from the UKNEQAS parasitology sub schemes. J Clin Pathol. 2003;56:927–32.CrossRefPubMedPubMedCentral Kettelhut M, Chiodini P, Edwards H, Moody A. External quality assessment schemes raise standards: evidence from the UKNEQAS parasitology sub schemes. J Clin Pathol. 2003;56:927–32.CrossRefPubMedPubMedCentral
5.
go back to reference WHO. Malaria microscopy quality assurance manual. Geneva: World Health Organization; 2009. WHO. Malaria microscopy quality assurance manual. Geneva: World Health Organization; 2009.
6.
go back to reference Annual East Wollega Zonal Health Department statistical report. June, 2014. Annual East Wollega Zonal Health Department statistical report. June, 2014.
7.
go back to reference Malaria laboratory diagnosis external quality assessment scheme guidelines. Ethiopian Federal Ministry of Health, September 2009. Malaria laboratory diagnosis external quality assessment scheme guidelines. Ethiopian Federal Ministry of Health, September 2009.
8.
go back to reference WHO-AFRO Laboratory Accreditation Assessment. Check list For Clinical and Public Health Laboratories- ISO 15189 document requirements for quality, and competence. 2012. WHO-AFRO Laboratory Accreditation Assessment. Check list For Clinical and Public Health Laboratories- ISO 15189 document requirements for quality, and competence. 2012.
9.
go back to reference Khan MA, Walley JD, Munir MA, Khan MA, Khobar NG, Tahir Z, et al. District level external quality assurance (EQA) of malaria microscopy in Pakistan: pilot implementation and feasibility. Malar J. 2011;10:45.CrossRefPubMedPubMedCentral Khan MA, Walley JD, Munir MA, Khan MA, Khobar NG, Tahir Z, et al. District level external quality assurance (EQA) of malaria microscopy in Pakistan: pilot implementation and feasibility. Malar J. 2011;10:45.CrossRefPubMedPubMedCentral
10.
go back to reference Hailegiorgis B, Girma S, Melaku Z, Teshi T, Demeke L, Gebresellasie S, et al. Laboratory malaria diagnostic capacity in health facilities in five administrative zones of Oromia Regional State, Ethiopia. Trop Med Int Health. 2010;15:1449–57.CrossRefPubMed Hailegiorgis B, Girma S, Melaku Z, Teshi T, Demeke L, Gebresellasie S, et al. Laboratory malaria diagnostic capacity in health facilities in five administrative zones of Oromia Regional State, Ethiopia. Trop Med Int Health. 2010;15:1449–57.CrossRefPubMed
11.
go back to reference Abreha T, Alemayehu B, Tadesse Y, Gebresillassie S, Tadesse A, et al. Malaria diagnostic capacity in health facilities in Ethiopia. Malar J. 2014;13:292.CrossRefPubMedPubMedCentral Abreha T, Alemayehu B, Tadesse Y, Gebresillassie S, Tadesse A, et al. Malaria diagnostic capacity in health facilities in Ethiopia. Malar J. 2014;13:292.CrossRefPubMedPubMedCentral
12.
go back to reference Zeleke B, Admasu G, Getachew T, Kebede E, Belay G, Abraha A, et al. External quality assessment of malaria microscopy in Hawassa health facilities, Southern Ethiopia. Clin Med Res. 2015;3:63–8.CrossRef Zeleke B, Admasu G, Getachew T, Kebede E, Belay G, Abraha A, et al. External quality assessment of malaria microscopy in Hawassa health facilities, Southern Ethiopia. Clin Med Res. 2015;3:63–8.CrossRef
13.
go back to reference Mukadi P, Gillet P, Lukuka A, Atua B, Sheshe N, Kanza A, et al. External quality assessment of Giemsa-stained blood film microscopy for the diagnosis of malaria and sleeping sickness in the Democratic Republic of the Congo. Bull World Health Organ. 2013;91:441–8.CrossRefPubMedPubMedCentral Mukadi P, Gillet P, Lukuka A, Atua B, Sheshe N, Kanza A, et al. External quality assessment of Giemsa-stained blood film microscopy for the diagnosis of malaria and sleeping sickness in the Democratic Republic of the Congo. Bull World Health Organ. 2013;91:441–8.CrossRefPubMedPubMedCentral
14.
go back to reference Frean J, Perovic O, Fensham V, McCarthy K, von Gottberg A, de Gouveia A, et al. External quality assessment of national public health laboratories in Africa, 2002–2009. Bull World Health Organ. 2012;90:191–9.CrossRefPubMedPubMedCentral Frean J, Perovic O, Fensham V, McCarthy K, von Gottberg A, de Gouveia A, et al. External quality assessment of national public health laboratories in Africa, 2002–2009. Bull World Health Organ. 2012;90:191–9.CrossRefPubMedPubMedCentral
15.
go back to reference Mitiku K, Mengistu G, Gelew B. The reliability of blood film examination for malaria at the peripheral health unit. Ethiop J Health Dev. 2003;17:197–204. Mitiku K, Mengistu G, Gelew B. The reliability of blood film examination for malaria at the peripheral health unit. Ethiop J Health Dev. 2003;17:197–204.
Metadata
Title
External quality assessment of malaria microscopy diagnosis in selected health facilities in Western Oromia, Ethiopia
Authors
Getachew Sori
Olifan Zewdie
Geletta Tadele
Abdi Samuel
Publication date
01-12-2018
Publisher
BioMed Central
Published in
Malaria Journal / Issue 1/2018
Electronic ISSN: 1475-2875
DOI
https://doi.org/10.1186/s12936-018-2386-2

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