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Cancer Cell International
Published in: Cancer Cell International 1/2016

Open Access 01-12-2016 | Primary research

Success of tumorsphere isolation from WHO grade IV gliomas does not correlate with the weight of fresh tumor specimens: an immunohistochemical characterization of tumorsphere differentiation

Authors: Kyoung Su Sung, Jin-Kyoung Shim, Ji-Hyun Lee, Se Hoon Kim, Sohee Park, Tae-Hoon Roh, Ju Hyung Moon, Eui-Hyun Kim, Sun Ho Kim, Su Jae Lee, Yong Min Huh, Seok-Gu Kang, Jong Hee Chang

Published in: Cancer Cell International | Issue 1/2016

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Abstract

Background

A trend of stage-by-stage increase in tumorsphere (TS) formation from glioma samples has been reported. Despite this trend, not all surgical specimens give rise to TSs, even World Health Organization (WHO) grade IV gliomas. Furthermore, it has been reported that differences in overall survival of primary glioblastoma patients depends on the propensity of their tumors to form TSs. However, the weights of fresh specimens vary from one surgical isolate to the next.

Methods

Accordingly, we evaluated the relationship between the weights of surgical specimens in WHO grade IV gliomas with the capacity to isolate TSs. Thirty-five fresh WHO grade IV glioma specimens were separated into two groups, based on whether they were positive or negative for TS isolation, and the relationship between TS isolation and weight of surgical specimens was assessed.

Results

We observed no significant difference in the weights of surgical samples in the two groups, and found that the optimal weight of specimens for TSs isolation was 500 mg.

Conclusion

Thus, contrary to our expectations, the ability to isolate TSs from WHO grade IV glioma specimens was not related to the weight of fresh specimens.
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Metadata
Title
Success of tumorsphere isolation from WHO grade IV gliomas does not correlate with the weight of fresh tumor specimens: an immunohistochemical characterization of tumorsphere differentiation
Authors
Kyoung Su Sung
Jin-Kyoung Shim
Ji-Hyun Lee
Se Hoon Kim
Sohee Park
Tae-Hoon Roh
Ju Hyung Moon
Eui-Hyun Kim
Sun Ho Kim
Su Jae Lee
Yong Min Huh
Seok-Gu Kang
Jong Hee Chang
Publication date
01-12-2016
Publisher
BioMed Central
Published in
Cancer Cell International / Issue 1/2016
Electronic ISSN: 1475-2867
DOI
https://doi.org/10.1186/s12935-016-0350-1

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