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BMC Complementary Medicine and Therapies

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Open Access 01-12-2019 | Helminths | Research article

Antiaging effect of a Jianpi-yangwei formula in Caenorhabditis elegans

Authors: Liling Zeng, Zhimin Yang, Tianchan Yun, Shaoyi Fan, Zhong Pei, Ziwen Chen, Chen Sun, Fuping Xu

Published in: BMC Complementary Medicine and Therapies | Issue 1/2019

Abstract

Background

Jianpi-yangwei (JPYW), a traditional Chinese medicine (TCM), helps to nourish the stomach and spleen and is primarily used to treat functional declines related to aging. This study aimed to explore the antiaging effects and mechanism of JPYW by employing a Caenorhabditis elegans model.

Methods

Wild-type C. elegans N2 worms were cultured in growth medium with or without JPYW, and lifespan analysis, oxidative and heat stress resistance assays, and other aging-related assays were performed. The effects of JPYW on the levels of superoxide dismutase (SOD) and the expression of specific genes were examined to explore the underlying mechanism of JPYW.

Results

Compared to control worms, JPYW-treated wild-type worms showed increased survival times under both normal and stress conditions (P < 0.05). JPYW-treated worms also exhibited enhanced reproduction, movement and growth and decreased intestinal lipofuscin accumulation compared to controls (P < 0.05). Furthermore, increased activity of SOD, downregulated expression levels of the proaging gene clk-2 and upregulated expression levels of the antiaging genes daf-16, skn-1, and sir-2.1 were observed in the JPYW group compared to the control group.

Conclusion

Our findings suggest that JPYW extends the lifespan of C. elegans and exerts antiaging effects by increasing the activity of an antioxidant enzyme (SOD) and by regulating the expression of aging-related genes. This study not only indicates that this Chinese compound exerts antiaging effects by activating and repressing target genes but also provides a proven methodology for studying the biological mechanisms of TCMs.

Harman D. Aging: a theory based on free radical and radiation chemistry. J Gerontol. 1956;11(3):298–300.PubMedCrossRef

MSN N, Longo-Mbenza B, Adeniyi OV, et al. Ageing, exposure to pollution, and interactions between climate change and local seasons as oxidant conditions predicting incident hematologic malignancy at KINSHASA University clinics, Democratic Republic of CONGO (DRC). BMC Cancer. 2017;17(1):559.CrossRef

Bijnens EM, Zeegers MP, Derom C, et al. Telomere tracking from birth to adulthood and residential traffic exposure. BMC Med. 2017;15(1):205.PubMedPubMedCentralCrossRef

Calder PC, Bosco N, Bourdet-Sicard R, et al. Health relevance of the modification of low grade inflammation in ageing (inflammageing) and the role of nutrition. Ageing Res Rev. 2017;40:95–119.PubMedCrossRef

Villa F, Carrizzo A, Spinelli CC, et al. Genetic analysis reveals a longevity-associated protein modulating endothelial function and angiogenesis. Circ Res. 2015;117(4):333–45.PubMedPubMedCentralCrossRef

Krut'ko VN, Dontsov VI, Khalyavkin AV, Markova AM. Natural aging as as a sequential poly-systemic syndrome. Front Biosci (Landmark Ed). 2018;23:909–20.CrossRef

Gladyshev TV, Gladyshev VN. A disease or not a disease? Aging as a pathology. Trends Mol Med. 2016;22(12):995–6.PubMedPubMedCentralCrossRef

Bellantuono I. Find drugs that delay many diseases of old age. Nature. 2018;554(7692):293–5.PubMedCrossRef

Savage N. New tricks from old dogs join the fight against ageing. Nature. 2017;552(7684):S57–9.PubMedCrossRef

10.

Liu J, Peng L, Huang W, et al. Balancing between aging and Cancer: molecular genetics meets traditional Chinese medicine. J Cell Biochem. 2017;118(9):2581–6.PubMedCrossRef

11.

Chen KJ. Reflections on human longevity and Chinese medicine prevention and treatment of chronic diseases. Chin J Integr Med. 2015;21(9):643–7.PubMedCrossRef

12.

Liang ZH, Yin DZ. Preventive treatment of traditional Chinese medicine as antistress and antiaging strategy. Rejuvenation Res. 2010;13(2–3):248–52.PubMedCrossRef

13.

Zhou DH. Preventive geriatrics: an overview from traditional Chinese medicine. Am J Chin Med. 1982;10(1–4):32–9.PubMedCrossRef

14.

Hsu YC, Chiu CJ, Wray LA, Beverly EA, Tseng SP. Impact of traditional Chinese medicine on age trajectories of health: evidence from the Taiwan longitudinal study on aging. J Am Geriatr Soc. 2015;63(2):351–7.PubMedCrossRef

15.

Wan F, Zhi D, Liu D, et al. Lifespan extension in Caenorhabiditis elegans by several traditional Chinese medicine formulas. Biogerontology. 2014;15(4):377–87.PubMedCrossRef

16.

Fan GR, Zong WJ, Wang XL. Effect of yishen jianpi drugs on T lymphocyte subsets, soluble interleukin-2 receptor and red blood cell immunity of senile deficiency syndrome patients. Zhongguo Zhong Xi Yi Jie He Za Zhi. 1995;15(1):18–20.PubMed

17.

Yang JY, Tao DQ, Liu S, Zhang S, Ma W, Shi ZH. Effects of three Wenyang Jianpi Tang on cell proliferation and apoptosis of nonalcoholic fatty liver cells. Zhongguo Zhong Yao Za Zhi. 2017;42(8):1591–6.PubMed

18.

Peng W, Zhang S, Zhang Z, et al. Jianpi Jiedu decoction, a traditional Chinese medicine formula, inhibits tumorigenesis, metastasis, and angiogenesis through the mTOR/HIF-1alpha/VEGF pathway. J Ethnopharmacol. 2018;224:140–8.PubMedCrossRef

19.

Huang C, Zhu Z, Cao X, et al. A Pectic Polysaccharide from Sijunzi Decoction Promotes the Antioxidant Defenses of SW480 Cells. Molecules. 2017;22(8):1341-12.PubMedCentralCrossRef

20.

Zhao AG, Zhao HL, Jin XJ, Yang JK, Tang LD. Effects of Chinese Jianpi herbs on cell apoptosis and related gene expression in human gastric cancer grafted onto nude mice. World J Gastroenterol. 2002;8(5):792–6.PubMedPubMedCentralCrossRef

21.

Gong YX, Sun Y, Lin AP. Comparative study on the anti-free-radical damage by vital energy-reinforcing method and blood-tonifying method. Zhongguo Zhong Yao Za Zhi. 1993;18(7):438–41 448.PubMed

22.

Shi XY, Zhao FZ, Dai X, Ma LS, Dong XY, Fang J. Effect of jianpiyiwei capsule on gastric precancerous lesions in rats. World J Gastroenterol. 2002;8(4):608–12.PubMedPubMedCentralCrossRef

23.

Guarente L, Kenyon C. Genetic pathways that regulate ageing in model organisms. Nature. 2000;408(6809):255–62.PubMedCrossRef

24.

Sluder AE, Baumeister R. From genes to drugs: target validation in Caenorhabditis elegans. Drug Discov Today Technol. 2004;1(2):171–7.PubMedCrossRef

25.

Munoz MJ, Riddle DL. Positive selection of Caenorhabditis elegans mutants with increased stress resistance and longevity. Genetics. 2003;163(1):171–80.PubMedPubMedCentral

26.

Zeng L, Sun C, Pei Z, et al. Liangyi Gao extends lifespan and exerts an antiaging effect in Caenorhabditis elegans by modulating DAF-16/FOXO. Biogerontology. 2019.

27.

Xian B, Shen J, Chen W, et al. WormFarm: a quantitative control and measurement device toward automated Caenorhabditis elegans aging analysis. Aging Cell. 2013;12(3):398–409.PubMedCrossRef

28.

Berdichevsky A, Nedelcu S, Boulias K, Bishop NA, Guarente L, Horvitz HR. 3-Ketoacyl thiolase delays aging of Caenorhabditis elegans and is required for lifespan extension mediated by sir-2.1. Proc Natl Acad Sci U S A. 2010;107(44):18927–32.PubMedPubMedCentralCrossRef

29.

Mukhopadhyay A, Oh SW, Tissenbaum HA. Worming pathways to and from DAF-16/FOXO. Exp Gerontol. 2006;41(10):928–34.PubMedCrossRef

30.

Chavez V, Mohri-Shiomi A, Maadani A, Vega LA, Garsin DA. Oxidative stress enzymes are required for DAF-16-mediated immunity due to generation of reactive oxygen species by Caenorhabditis elegans. Genetics. 2007;176(3):1567–77.PubMedPubMedCentralCrossRef

31.

Yang Y, Ren C, Zhang Y, Wu X. Ginseng: an nonnegligible natural remedy for healthy aging. Aging Dis. 2017;8(6):708–20.PubMedPubMedCentralCrossRef

32.

Lee JH, Choi SH, Kwon OS, et al. Effects of ginsenosides, active ingredients of Panax ginseng, on development, growth, and life span of Caenorhabditis elegans. Biol Pharm Bull. 2007;30(11):2126–34.PubMedCrossRef

33.

Hu W, Jing P, Wang L, Zhang Y, Yong J, Wang Y. The positive effects of Ginsenoside Rg1 upon the hematopoietic microenvironment in a D-Galactose-induced aged rat model. BMC Complement Altern Med. 2015;15:119.PubMedPubMedCentralCrossRef

34.

Zhou Y, Liu J, Cai S, Liu D, Jiang R, Wang Y. Protective effects of ginsenoside Rg1 on aging Sca-1(+) hematopoietic cells. Mol Med Rep. 2015;12(3):3621–8.PubMedCrossRef

35.

Lee SG, Kim MM. Pachymic acid promotes induction of autophagy related to IGF-1 signaling pathway in WI-38 cells. Phytomedicine. 2017;36:82–7.PubMedCrossRef

36.

Bi J, Zhang H, Lu J, Lei W. Nobiletin ameliorates isoflurane-induced cognitive impairment via antioxidant, anti-inflammatory and anti-apoptotic effects in aging rats. Mol Med Rep. 2016;14(6):5408–14.PubMedCrossRef

37.

Yu YB, Dosanjh L, Lao L, Tan M, Shim BS, Luo Y. Cinnamomum cassia bark in two herbal formulas increases life span in Caenorhabditis elegans via insulin signaling and stress response pathways. PLoS One. 2010;5(2):e9339.PubMedPubMedCentralCrossRef

38.

Rho HS, Hong SH, Park J, et al. Kojyl cinnamate ester derivatives promote adiponectin production during adipogenesis in human adipose tissue-derived mesenchymal stem cells. Bioorg Med Chem Lett. 2014;24(9):2141–5.PubMedCrossRef

39.

Finkel T, Holbrook NJ. Oxidants, oxidative stress and the biology of ageing. Nature. 2000;408(6809):239–47.PubMedCrossRef

40.

Bokov A, Chaudhuri A, Richardson A. The role of oxidative damage and stress in aging. Mech Ageing Dev. 2004;125(10–11):811–26.PubMedCrossRef

41.

Murphy CT, McCarroll SA, Bargmann CI, et al. Genes that act downstream of DAF-16 to influence the lifespan of Caenorhabditis elegans. Nature. 2003;424(6946):277–83.PubMedCrossRef

42.

Tissenbaum HA, Guarente L. Increased dosage of a sir-2 gene extends lifespan in Caenorhabditis elegans. Nature. 2001;410(6825):227–30.PubMedCrossRef

43.

Berdichevsky A, Viswanathan M, Horvitz HR, Guarente L. C. elegans SIR-2.1 interacts with 14-3-3 proteins to activate DAF-16 and extend life span. Cell. 2006;125(6):1165–77.PubMedCrossRef

44.

Park SK, Tedesco PM, Johnson TE. Oxidative stress and longevity in Caenorhabditis elegans as mediated by SKN-1. Aging Cell. 2009;8(3):258–69.PubMedCrossRef

45.

Benard C, McCright B, Zhang Y, Felkai S, Lakowski B, Hekimi S. The C. Elegans maternal-effect gene clk-2 is essential for embryonic development, encodes a protein homologous to yeast Tel2p and affects telomere length. Development. 2001;128(20):4045–55.PubMed

Title: Antiaging effect of a Jianpi-yangwei formula in Caenorhabditis elegans
Authors: Liling Zeng
Zhimin Yang
Tianchan Yun
Shaoyi Fan
Zhong Pei
Ziwen Chen
Chen Sun
Fuping Xu
Publication date: 01-12-2019
Publisher: BioMed Central
Keyword: Helminths
Published in: BMC Complementary Medicine and Therapies / Issue 1/2019
Electronic ISSN: 2662-7671
DOI: https://doi.org/10.1186/s12906-019-2704-4

Keynote webinar | Spotlight on sleep in brain health

Springer Medicine

Antiaging effect of a Jianpi-yangwei formula in Caenorhabditis elegans

Abstract

Background

Methods

Results

Conclusion

Keynote webinar | Spotlight on sleep in brain health

Springer Medicine

Abstract

Background

Methods

Results

Conclusion

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