Skip to main content
Key Specialties
Cardiology Diabetology Endocrinology Gastroenterology Geriatrics and Gerontology Gynecology and Obstetrics Hematology Infectious Disease Internal Medicine Nephrology Neurology Oncology Pediatrics Respiratory Medicine Rheumatology Surgery
Congress Coverage
2024 ACC Congress 2023 ESMO Congress 2023 EASD Congress 2023 ERS Congress 2023 ESC Congress 2023 EHA Congress 2023 ASCO Annual Meeting
Clinical Collections
Advances in Alzheimer’s

Keynote webinar | Spotlight on sleep in brain health

LIVE: Thursday 2nd May 2024, 18:00-19:30 (CEST)

Quality sleep is essential for health. But what happens to our brains when sleep patterns are disturbed? Join our experts to explore the interplay between sleep disruption and neurological diseases, and the questions that you need to be asking your patients to help you prevent the harmful effects of sleep deprivation.
ADVANCED SEARCH
Log in
Top
BMC Complementary Medicine and Therapies
Published in: BMC Complementary Medicine and Therapies 1/2018

Open Access 01-12-2018 | Research article

Rhaponticum acaule (L) DC essential oil: chemical composition, in vitro antioxidant and enzyme inhibition properties

Authors: Habib Mosbah, Hassiba Chahdoura, Jannet Kammoun, Malek Besbes Hlila, Hanen Louati, Saoussen Hammami, Guido Flamini, Lotfi Achour, Boulbaba Selmi

Published in: BMC Complementary Medicine and Therapies | Issue 1/2018

Login to get access

Abstract

Background

α-glucosidase is a therapeutic target for diabetes mellitus (DM) and α-glucosidase inhibitors play a vital role in the treatments for the disease. Furthermore, xanthine oxidase (XO) is a key enzyme that catalyzes hypoxanthine and xanthine to uric acid which at high levels can lead to hyperuricemia which is an important cause of gout. Pancreatic lipase (PL) secreted into the duodenum plays a key role in the digestion and absorption of fats. For its importance in lipid digestion, PL represents an attractive target for obesity prevention.

Methods

The flowers essential oil of Rhaponticum acaule (L) DC (R. acaule) was characterized using gas chromatography-mass spectrometry (GC-MS). The antioxidant activities of R. acaule essential oil (RaEO) were also determined using 2,2’-azinobis-3-ethylbenzothiazoline-6-sulfonic acid (ABTS), reducing power, phosphomolybdenum, and DNA nicking assays. The inhibitory power of RaEO against α-glucosidase, xanthine oxidase and pancreatic lipase was evaluated. Enzyme kinetic studies using Michaelis-Menten and the derived Lineweaver-Burk (LB) plots were performed to understand the possible mechanism of inhibition exercised by the components of this essential oil.

Results

The result revealed the presence of 26 compounds (97.4%). The main constituents include germacrene D (49.2%), methyl eugenol (8.3%), (E)-β-ionone (6.2%), β-caryophyllene (5.7%), (E,E)-α-farnesene (4.2%), bicyclogermacrene (4.1%) and (Z)-α-bisabolene (3.7%). The kinetic inhibition study showed that the essential oil demonstrated a strong α-glucosidase inhibiton and it was a mixed inhibitor. On the other hand, our results evidenced that this oil exhibited important xanthine oxidase inhibitory effect, behaving as a non-competitive inhibitor. The essential oil inhibited the turkey pancreatic lipase, with maximum inhibition of 80% achieved at 2 mg/mL. Furthermore, the inhibition of turkey pancreatic lipase by RaEO was an irreversible one.

Conclusion

The results revealed that the RaEO is a new promising potential source of antioxidant compounds, endowed with good practical applications for human health.
Literature
1.
Bakkali F, Averbeck S, Averbeck D, Idaomar M. Biological effects of essential oils–a review. Food Chem Toxicol. 2008;46(2):446–75.CrossRefPubMed
2.
Zhang D-Y, Yao X-H, Duan M-H, Wei F-Y, Wu G-H, Li L. Variation of essential oil content and antioxidant activity of Lonicera species in different sites of China. Ind Crop Prod. 2015;77:772–9.CrossRef
3.
Loizzo MR, Menichini F, Tundis R, Bonesi M, Conforti F, Nadjafi F, Statti GA, Frega NG, Menichini F. In vitro biological activity of Salvia leriifolia Benth essential oil relevant to the treatment of Alzheimer’s disease. J Oleo Sci. 2009;58(8):443–6.CrossRefPubMed
4.
Loizzo MR, Jemia MB, Senatore F, Bruno M, Menichini F, Tundis R. Chemistry and functional properties in prevention of neurodegenerative disorders of five cistus species essential oils. Food Chem Toxicol. 2013;59:586–94.CrossRefPubMed
5.
Tongnuanchan P, Benjakul S. Essential oils: extraction, bioactivities, and their uses for food preservation. J Food Sci. 2014;79(7):R1231–49.CrossRefPubMed
6.
Yanishlieva NV, Marinova E, Pokorný J. Natural antioxidants from herbs and spices. Eur J Lipid Sci Technol. 2006;108(9):776–93.CrossRef
7.
Souza E, Stamford T, Lima E, Trajano V. Effectiveness of Origanum vulgare L. essential oil to inhibit the growth of food spoiling yeasts. Food Control. 2007;18(5):409–13.CrossRef
8.
Adorjan B, Buchbauer G. Biological properties of essential oils: an updated review. Flavour Fragrance J. 2010;25(6):407–26.CrossRef
9.
Buchbauer G, Jirovetz L, Jager W, Plank C, Dietrich H. Fragrance compounds and essential oils with sedative effects upon inhalation. J Pharm Sci. 1993;82(6):660–4.CrossRefPubMed
10.
Franz CM. Essential oil research: past, present and future. Flavour Fragrance J. 2010;25(3):112–3.CrossRef
11.
Firenzuoli F, Jaitak V, Horvath G, Bassolé IHN, Setzer WN, Gori L. Essential oils: new perspectives in human health and wellness. Evid Based Complement Alternat Med. 2014;2014:2.CrossRef
12.
Abad MJ, Bedoya LM, Bermejo P. Essential oils from the Asteraceae family active against multidrug-resistant bacteria. In: Fighting multidrug resistance with herbal extracts, essential oils and their component. edn. London: Academic Press; 2013. p. 205–19.CrossRef
13.
Zhang YH, Cheng JK, Yang L, Cheng DL. Triterpenoids from Rhaponticum uniflorum. J Chin Chem Soc. 2002;49(1):117–24.CrossRef
14.
Petkov V, Roussinov K, Todorov S, Lazarova M, Yonkov D, Draganova S. Pharmacological investigations on Rhaponticum carthamoides. Planta Med. 1984;50(03):205–9.CrossRefPubMed
15.
Kokoska L, Janovska D. Chemistry and pharmacology of Rhaponticum carthamoides: a review. Phytochemistry. 2009;70(7):842–55.CrossRefPubMed
16.
Boussaada O, Ammar S, Saidana D, Chriaa J, Chraif I, Daami M, Helal A, Mighri Z. Chemical composition and antimicrobial activity of volatile components from capitula and aerial parts of Rhaponticum acaule DC growing wild in Tunisia. Microbiol Res. 2008;163(1):87–95.CrossRefPubMed
17.
Denny EFK. Hydro-distillation of oils from aromatic herbs. Perfum Flavor. 1989;14:57–63.
18.
Masada Y: Analysis of essential oils by gas chromatography and mass spectrometry. 1976.
19.
Jennings W. Qualitative analysis of flavor and fragrance volatiles by glass capillary gas chromatography. New York: Academic Press; 2012.
20.
Davies NW. Gas chromatographic retention indices of monoterpenes and sesquiterpenes on methyl silicon and Carbowax 20M phases. J Chromatogr A. 1990;503:1–24.CrossRef
21.
Adams RP. Identification of essential oil components by gas chromatography/mass spectrometry. 4th ed. Carol Stream: Allured Publ.; 2007.
22.
Re R, Pellegrini N, Proteggente A, Pannala A, Yang M, Rice-Evans C. Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radic Biol Med. 1999;26(9):1231–7.CrossRefPubMed
23.
Oyaizu M. Studies on products of browning reaction. Japanese J Nutr Diet. 1986;44(6):307–15.CrossRef
24.
Prieto P, Pineda M, Aguilar M. Spectrophotometric quantitation of antioxidant capacity through the formation of a phosphomolybdenum complex: specific application to the determination of vitamin E. Anal Biochem. 1999;269(2):337–41.CrossRefPubMed
25.
Lee J-C, Kim H-R, Kim J, Jang Y-S. Antioxidant property of an ethanol extract of the stem of Opuntia ficus-indica var. saboten. J Agric Food Chem. 2002;50(22):6490–6.CrossRefPubMed
26.
Tao Y, Zhang Y, Cheng Y, Wang Y. Rapid screening and identification of α-glucosidase inhibitors from mulberry leaves using enzyme-immobilized magnetic beads coupled with HPLC/MS and NMR. Biomed Chromatogr. 2013;27(2):148–55.CrossRefPubMed
27.
Rengasamy KR, Aderogba MA, Amoo SO, Stirk WA, Van Staden J. Potential antiradical and alpha-glucosidase inhibitors from Ecklonia maxima (Osbeck) Papenfuss. Food Chem. 2013;141(2):1412–5.CrossRefPubMed
28.
Kong L, Cai Y, Huang W, Cheng CH, Tan R. Inhibition of xanthine oxidase by some Chinese medicinal plants used to treat gout. J Ethnopharmacol. 2000;73(1):199–207.CrossRefPubMed
29.
Rathelot J, Julien R, Canioni P, Coeroli C, Sarda L. Studies on the effect of bile salt and colipase on enzymatic lipolysis. Improved method for the determination of pancreatic lipase and colipase. Biochimie. 1975;57(10):1117–22.CrossRefPubMed
30.
Gounder DK, Lingamallu J. Comparison of chemical composition and antioxidant potential of volatile oil from fresh, dried and cured turmeric (Curcuma longa) rhizomes. Ind Crop Prod. 2012;38:124–31.CrossRef
31.
Shimada K, Fujikawa K, Yahara K, Nakamura T. Antioxidative properties of xanthan on the autoxidation of soybean oil in cyclodextrin emulsion. J Agric Food Chem. 1992;40(6):945–8.CrossRef
32.
Murata K, Nakao K, Hirata N, Namba K, Nomi T, Kitamura Y, Moriyama K, Shintani T, Iinuma M, Matsuda H. Hydroxychavicol: a potent xanthine oxidase inhibitor obtained from the leaves of betel, Piper betle. J Nat Med. 2009;63(3):355–9.CrossRefPubMed
33.
Fendri A, Sayari A, Gargouri Y. Kinetic properties of Turkey pancreatic lipase: a comparative study with emulsified tributyrin and monomolecular dicaprin. Chirality. 2005;17(1):57–62.CrossRefPubMed
34.
Skała E, Rijo P, Garcia C, Sitarek P, Kalemba D, Toma M, Szemraj J, Pytel D, Wysokińska H, Śliwiński T. The Essential Oils of Rhaponticum carthamoides Hairy Roots and Roots of Soil-Grown Plants: Chemical Composition and Antimicrobial, Anti-Inflammatory, and Antioxidant Activities. Oxidative Med Cell Longev. 2016;2016:1–10.
35.
Havlik J, Budesinsky M, Kloucek P, Kokoska L, Valterova I, Vasickova S, Zeleny V. Norsesquiterpene hydrocarbon, chemical composition and antimicrobial activity of Rhaponticum carthamoides root essential oil. Phytochemistry. 2009;70(3):414–8.CrossRefPubMed
36.
Benyelles B, Allali H, Dib MEA, Djabou N, Tabti B, Costa J. Essential oil from Rhaponticum acaule L. roots: comparative study using HS-SPME/GC/GC–MS and hydrodistillation techniques. J Saudi Chem Soc. 2014;18(6):972–6.CrossRef
37.
Filizadeh Y, Goodarzi G. Essential oils from hairy root cultures and field cultivated roots of valerian (Valeriana sisymbriifolium). J Med Plant Res. 2010;9:120–8.
38.
Zhang L, Zhang X-Q, Sun M. Comparative analysis of the essential oils from normal and hairy roots of Panax japonicas CA Meyer. Afr J Biotechnol. 2011;10(13):2440–5.
39.
Afoulous S, Ferhout H, Raoelison EG, Valentin A, Moukarzel B, Couderc F, Bouajila J. Chemical composition and anticancer, antiinflammatory, antioxidant and antimalarial activities of leaves essential oil of Cedrelopsis grevei. Food Chem Toxicol. 2013;56:352–62.CrossRefPubMed
40.
Mattazi N, Farah A, Fadil M, Chraibi M, Benbrahim K. Essential oils analysis and antibacterial activity of the leaves of Rosmarinus officinalis, Salvia officinalis and Mentha piperita cultivated in Agadir (Morocco). Int J Pharm Pharm Sci. 2015;7(9):73–9.
41.
42.
Maisuthisakul P, Suttajit M, Pongsawatmanit R. Assessment of phenolic content and free radical-scavenging capacity of some Thai indigenous plants. Food Chem. 2007;100:1409–18.CrossRef
43.
Kaurinovic B, Vlaisavljevic S, Popovic M, Vastag D, Djurendic-Brenesel M. Antioxidant properties of Marrubium peregrinum L. (Lamiaceae) essential oil. Molecules. 2010;15(9):5943–55.CrossRef
44.
Rajabian A, Khayyat MH, Emami SA, Tayarani-Najaran Z, Oskooie RR, Asili J: Phytochemical evaluation and antioxidant activity of essential oil, and aqueous and organic extracts of Artemisia dracunculus. 2016.
45.
Johnson TO, Ermolieff J, Jirousek MR. Protein tyrosine phosphatase 1B inhibitors for diabetes. Nat Rev Drug Discov. 2002;1(9):696–709.CrossRefPubMed
46.
Reddy NVLS, Anarthe SJ, Raghavendra NM. In vitro antioxidant and antidiabetic activity of Asystasia gangetica (Chinese violet) Linn. (Acanthaceae). J Res Biomed Sci. 2011;1(2):72–5.
47.
Sarikurkcu C, Uren MC, Kocak MS, Cengiz M, Tepe B. Chemical composition, antioxidant, and enzyme inhibitory activities of the essential oils of three Phlomis species as well as their fatty acid compositions. Food Sci Biotechnol. 2016;25(3):687–93.CrossRef
48.
Afifi FU, Kasabri V, Al-Jaber HI, Abu-Irmaileh BE, Al-Qudah MA, Abazaa I. Composition and biological activity of volatile oil from Salvia judaica and S. multicaulis from Jordan. Nat Prod Commun. 2016;11(4):535.PubMed
49.
Gargouri Y, Chahinian H, Moreau H, Ransac S, Verger R. Inactivation of pancreatic and gastric lipases by THL and C12: 0-TNB: a kinetic study with emulsified tributyrin. Biochim Biophys Acta. 1991;1085(3):322–8.CrossRefPubMed
50.
Gargouri Y, Pieroni G, Rivière C, Sugihara A, Sarda L, Verger R. Inhibition of lipases by proteins. A kinetic study with dicaprin monolayers. J Biol Chem. 1985;260(4):2268–73.PubMed
51.
Gargouri Y, Pieroni G, Riviere C, Sarda L, Verger R. Inhibition of lipases by proteins: a binding study using dicaprin monolayers. Biochemistry. 1986;25(7):1733–8.CrossRefPubMed
Metadata
Title
Rhaponticum acaule (L) DC essential oil: chemical composition, in vitro antioxidant and enzyme inhibition properties
Authors
Habib Mosbah
Hassiba Chahdoura
Jannet Kammoun
Malek Besbes Hlila
Hanen Louati
Saoussen Hammami
Guido Flamini
Lotfi Achour
Boulbaba Selmi
Publication date
01-12-2018
Publisher
BioMed Central
Published in
BMC Complementary Medicine and Therapies / Issue 1/2018
Electronic ISSN: 2662-7671
DOI
https://doi.org/10.1186/s12906-018-2145-5

Other articles of this Issue 1/2018

BMC Complementary Medicine and Therapies 1/2018 Go to the issue

Research article

Concurrent regulation of LKB1 and CaMKK2 in the activation of AMPK in castrate-resistant prostate cancer by a well-defined polyherbal mixture with anticancer properties

Research article

Antiproliferative activities and phenolic acid content of water and ethanolic extracts of the powdered formula of Houttuynia cordata Thunb. fermented broth and Phyllanthus emblica Linn. fruit

Research article

Stimulatory effect of icariin on the proliferation of neural stem cells from rat hippocampus

Research article

Curcumin derivative, 2,6-bis(2-fluorobenzylidene)cyclohexanone (MS65) inhibits interleukin-6 production through suppression of NF-κB and MAPK pathways in histamine-induced human keratinocytes cell (HaCaT)

Research article

Emotional release and physical symptom improvement: a qualitative analysis of self-reported outcomes and mechanisms in patients treated with neural therapy

Research article

Antibacterial efficacy of leaf extracts of Combretum album Pers. against some pathogenic bacteria