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BMC Complementary Medicine and Therapies
Published in: BMC Complementary Medicine and Therapies 1/2017

Open Access 01-12-2017 | Research article

The protective effects of Resveratrol against radiation-induced intestinal injury

Authors: Heng Zhang, Hao Yan, Xiaoliang Zhou, Huaqing Wang, Yiling Yang, Junling Zhang, Hui Wang

Published in: BMC Complementary Medicine and Therapies | Issue 1/2017

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Abstract

Background

Intestinal injury is a potential cause of death after high-dose radiation exposure. The aim of the present study was to investigate the protective effects of resveratrol against radiation-induced small intestine injury.

Methods

C57BL/6 N mice were irradiated and treated with resveratrol and/or Ex527 (a potent Sirt1 inhibitor), and subsequent examining intestinal morphological changes, and crypt cell apoptosis. Then, the expression and enzyme activity of SOD2 in the small intestine were examined. Furthermore, Sirt1 and acetylated p53 expression was analysed.

Results

Compared to the vehicle control, treatment with resveratrol improved intestinal morphology, decreased apoptosis of crypt cells, maintained cell regeneration, and ameliorated SOD2 expression and activity. Resveratrol also regulated Sirt1 and acetylated p53 expression perturbed by irradiation in the small intestine. The protective effect of resveratrol against ionizing radiation induced small intestine injury was significantly inhibited by Ex527.

Conclusion

Our results suggest that resveratrol decreases the effects of radiation on intestinal injury at least partly via activation of Sirt1.
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Literature
1.
Thomas GM. Concurrent chemotherapy and radiation for locally advanced cervical cancer: the new standard of care. Semin Radiat Oncol. 2000;10(1):44–50.CrossRefPubMed
2.
Hauer-Jensen M, Wang J, Denham JW. Bowel injury: current and evolving management strategies. Semin Radiat Oncol. 2003;13(3):357–71.PubMed
3.
Wang J, Zheng H, Ou X, Albertson CM, Fink LM, Herbert JM, Hauer-Jensen M. Hirudin ameliorates intestinal radiation toxicity in the rat: support for thrombin inhibition as strategy to minimize side-effects after radiation therapy and as countermeasure against radiation exposure. J Thromb Haemostasis : JTH. 2004;2(11):2027–35.CrossRef
4.
Monti P, Wysocki J, van der Meeren A, Griffiths NM. The contribution of radiation-induced injury to the gastrointestinal tract in the development of multi-organ dysfunction syndrome or failure. BJR Suppl BIR. 2005;27:89–94.CrossRef
5.
Hirama T, Tanosaki S, Kandatsu S, Kuroiwa N, Kamada T, Tsuji H, Yamada S, Katoh H, Yamamoto N, Tsujii H, et al. Initial medical management of patients severely irradiated in the Tokai-mura criticality accident. Br J Radiol. 2003;76(904):246–53.CrossRefPubMed
6.
Das S, Das DK. Anti-inflammatory responses of resveratrol. Inflamm Allergy Drug Targets. 2007;6(3):168–73.CrossRefPubMed
7.
Bradamante S, Barenghi L, Villa A. Cardiovascular protective effects of resveratrol. Cardiovasc Drug Rev. 2004;22(3):169–88.CrossRefPubMed
8.
de la Lastra CA, Villegas I. Resveratrol as an anti-inflammatory and anti-aging agent: mechanisms and clinical implications. Mol Nutr Food Res. 2005;49(5):405–30.CrossRefPubMed
9.
Pervaiz S, Holme AL. Resveratrol: its biologic targets and functional activity. Antioxid Redox Signal. 2009;11(11):2851–97.CrossRefPubMed
10.
Zhang H, Zhai Z, Wang Y, Zhang J, Wu H, Li C, Li D, Lu L, Wang X, Chang J, et al. Resveratrol ameliorates ionizing irradiation-induced long-term hematopoietic stem cell injury in mice. Free Radic Biol Med. 2013;54:40–50.CrossRefPubMed
11.
12.
13.
Saunders LR, Verdin E. Sirtuins: critical regulators at the crossroads between cancer and aging. Oncogene. 2007;26(37):5489–504.CrossRefPubMed
14.
Ling S, Li J, Shan Q, Dai H, Lu D, Wen X, Song P, Xie H, Zhou L, Liu J, et al. USP22 mediates the multidrug resistance of hepatocellular carcinoma via the SIRT1/AKT/MRP1 signaling pathway. Mol Oncol. 2017;11(6):682–95.CrossRefPubMedPubMedCentral
15.
Wang X, Yang B, Ma B. The UCA1/miR-204/Sirt1 axis modulates docetaxel sensitivity of prostate cancer cells. Cancer Chemother Pharmacol. 2016;78(5):1025–31.CrossRefPubMed
16.
Xie Y, Zhang J, Ye S, He M, Ren R, Yuan D, Shao C. SirT1 regulates radiosensitivity of hepatoma cells differently under normoxic and hypoxic conditions. Cancer Sci. 2012;103(7):1238–44.CrossRefPubMed
17.
Fu Y, Wang Y, Du L, Xu C, Cao J, Fan T, Liu J, Su X, Fan S, Liu Q, et al. Resveratrol inhibits ionising irradiation-induced inflammation in MSCs by activating SIRT1 and limiting NLRP-3 inflammasome activation. Int J Mol Sci. 2013;14(7):14105–18.CrossRefPubMedPubMedCentral
18.
Said RS, El-Demerdash E, Nada AS, Kamal MM. Resveratrol inhibits inflammatory signaling implicated in ionizing radiation-induced premature ovarian failure through antagonistic crosstalk between silencing information regulator 1 (SIRT1) and poly(ADP-ribose) polymerase 1 (PARP-1). Biochem Pharmacol. 2016;103:140–50.CrossRefPubMed
19.
Booth C, Booth D, Williamson S, Demchyshyn LL, Potten CS. Teduglutide ([Gly2]GLP-2) protects small intestinal stem cells from radiation damage. Cell Prolif. 2004;37(6):385–400.CrossRefPubMed
20.
Driak D, Osterreicher J, Vavrova J, Rehakova Z, Vilasova Z. Morphological changes of rat jejunum after whole body gamma-irradiation and their impact in biodosimetry. Physiol Res Academia Sci Bohemoslov. 2008;57(3):475–9.
21.
Zhang H, Li J, Wang YY, Meng AM, Liu Q, Wang L, Chen FH, Wang XC, Zhai ZB, Fu Y, et al. Retinoblastoma 94 enhances radiation treatment of esophageal squamous cell carcinoma in vitro and in vivo. J Radiat Res. 2012;53(1):117–24.CrossRefPubMed
22.
Kim JS, Ryoo SB, Heo K, Kim JG, Son TG, Moon C, Yang K. Attenuating effects of granulocyte-colony stimulating factor (G-CSF) in radiation induced intestinal injury in mice. Food and Chem Toxicol Int J Published British Ind Biol Res Assoc. 2012;50(9):3174–80.CrossRef
23.
Kim GJ, Chandrasekaran K, Morgan WF. Mitochondrial dysfunction, persistently elevated levels of reactive oxygen species and radiation-induced genomic instability: a review. Mutagenesis. 2006;21(6):361–7.CrossRefPubMed
24.
Milne JC, Denu JM. The Sirtuin family: therapeutic targets to treat diseases of aging. Curr Opin Chem Biol. 2008;12(1):11–7.CrossRefPubMed
25.
Napper AD, Hixon J, McDonagh T, Keavey K, Pons JF, Barker J, Yau WT, Amouzegh P, Flegg A, Hamelin E, et al. Discovery of indoles as potent and selective inhibitors of the deacetylase SIRT1. J Med Chem. 2005;48(25):8045–54.CrossRefPubMed
26.
Potten CS, Grant HK. The relationship between ionizing radiation-induced apoptosis and stem cells in the small and large intestine. Br J Cancer. 1998;78(8):993–1003.CrossRefPubMedPubMedCentral
27.
Ishihara H, Tanaka I, Yakumaru H, Tanaka M, Yokochi K, Akashi M. Pharmaceutical drugs supporting regeneration of small-intestinal mucosa severely damaged by ionizing radiation in mice. J Radiat Res. 2013;54(6):1057–64.CrossRefPubMedPubMedCentral
28.
Szabo S, Sandor Z, Vincze A, Gombos Z, Mohiuddin A, Viravathana T. Radiation-induced enterocolitis: basic and applied science. Eur J Surg Suppl Acta Chir Suppl. 1998;582:85–9.
29.
Ishihara H, Tanaka I, Yakumaru H, Tanaka M, Satoh A, Ishiwata A, Yokochi K, Kurematsu A, Ueda J, Shibata T, et al. Acceleration of regeneration of mucosa in small intestine damaged by ionizing radiation using anabolic steroids. Radiat Res. 2011;175(3):367–74.CrossRefPubMed
30.
Kao CL, Chen LK, Chang YL, Yung MC, Hsu CC, Chen YC, Lo WL, Chen SJ, Ku HH, Hwang SJ. Resveratrol protects human endothelium from H(2)O(2)-induced oxidative stress and senescence via SirT1 activation. J Atheroscler Thromb. 2010;17(9):970–9.CrossRefPubMed
31.
Anekonda TS, Adamus G. Resveratrol prevents antibody-induced apoptotic death of retinal cells through upregulation of Sirt1 and Ku70. BMC Res Notes. 2008;1:122.CrossRefPubMedPubMedCentral
32.
Bass TM, Weinkove D, Houthoofd K, Gems D, Partridge L. Effects of resveratrol on lifespan in Drosophila Melanogaster and Caenorhabditis Elegans. Mech Ageing Dev. 2007;128(10):546–52.CrossRefPubMed
33.
34.
Aquilano K, Baldelli S, Pagliei B, Cannata SM, Rotilio G, Ciriolo MR. p53 orchestrates the PGC-1alpha-mediated antioxidant response upon mild redox and metabolic imbalance. Antioxid Redox Signal. 2013;18(4):386–99.CrossRefPubMedPubMedCentral
35.
36.
Lagouge M, Argmann C, Gerhart-Hines Z, Meziane H, Lerin C, Daussin F, Messadeq N, Milne J, Lambert P, Elliott P, et al. Resveratrol improves mitochondrial function and protects against metabolic disease by activating SIRT1 and PGC-1alpha. Cell. 2006;127(6):1109–22.CrossRefPubMed
37.
Shih AY, Johnson DA, Wong G, Kraft AD, Jiang L, Erb H, Johnson JA, Murphy TH. Coordinate regulation of glutathione biosynthesis and release by Nrf2-expressing glia potently protects neurons from oxidative stress. J Neurosci Official J Society Neurosci. 2003;23(8):3394–406.
38.
Travis EL, Rachakonda G, Zhou X, Korhonen K, Sekhar KR, Biswas S, Freeman ML. NRF2 deficiency reduces life span of mice administered thoracic irradiation. Free Radic Biol Med. 2011;51(6):1175–83.CrossRefPubMedPubMedCentral
39.
He X, Wang L, Szklarz G, Bi Y, Ma Q. Resveratrol inhibits paraquat-induced oxidative stress and fibrogenic response by activating the nuclear factor erythroid 2-related factor 2 pathway. J Pharmacol Exp Ther. 2012;342(1):81–90.CrossRefPubMedPubMedCentral
40.
Huang EY, Wang FS, Lin IH, Yang KD. Aminoguanidine alleviates radiation-induced small-bowel damage through its antioxidant effect. Int J Radiat Oncol Biol Phys. 2009;74(1):237–44.CrossRefPubMed
41.
Harris JW, Phillips TL. Radiobiolgoical and biochemical studies of thiophosphate radioprotective compounds related to cysteamine. Radiat Res. 1971;46(2):362–79.CrossRefPubMed
42.
Huang EY, Wang FS, Chen YM, Chen YF, Wang CC, Lin IH, Huang YJ, Yang KD. Amifostine alleviates radiation-induced lethal small bowel damage via promotion of 14-3-3sigma-mediated nuclear p53 accumulation. Oncotarget. 2014;5(20):9756–69.CrossRefPubMedPubMedCentral
43.
Milas L, Hunter N, Reid BO, Thames HD Jr. Protective effects of S-2-(3-aminopropylamino)ethylphosphorothioic acid against radiation damage of normal tissues and a fibrosarcoma in mice. Cancer Res. 1982;42(5):1888–97.PubMed
44.
Carsten RE, Bachand AM, Bailey SM, Ullrich RL. Resveratrol reduces radiation-induced chromosome aberration frequencies in mouse bone marrow cells. Radiat Res. 2008;169(6):633–8.CrossRefPubMedPubMedCentral
45.
Yang Y, Paik JH, Cho D, Cho JA, Kim CW. Resveratrol induces the suppression of tumor-derived CD4+CD25+ regulatory T cells. Int Immunopharmacol. 2008;8(4):542–7.CrossRefPubMed
46.
Metadata
Title
The protective effects of Resveratrol against radiation-induced intestinal injury
Authors
Heng Zhang
Hao Yan
Xiaoliang Zhou
Huaqing Wang
Yiling Yang
Junling Zhang
Hui Wang
Publication date
01-12-2017
Publisher
BioMed Central
Published in
BMC Complementary Medicine and Therapies / Issue 1/2017
Electronic ISSN: 2662-7671
DOI
https://doi.org/10.1186/s12906-017-1915-9

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