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BMC Complementary Medicine and Therapies
Published in: BMC Complementary Medicine and Therapies 1/2017

Open Access 01-12-2017 | Research article

Effect of solvents extraction on phytochemical components and biological activities of Tunisian date seeds (var. Korkobbi and Arechti)

Authors: Amira Thouri, Hassiba Chahdoura, Amira El Arem, Amel Omri Hichri, Rihab Ben Hassin, Lotfi Achour

Published in: BMC Complementary Medicine and Therapies | Issue 1/2017

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Abstract

Background

The interest in natural antioxidants, especially polyphenols, is growing more and more thanks to their positive contribution to human health. Thus, the prevention from the harmful action of oxidative stress which has been involved in many diseases such as cancer, inflammation diabetes, and cardiovascular illness.
Recent research proved the bioactive compounds richness of date seeds which could be a good biological matrix of natural antioxidants. Unfortunately, an important quantity of Tunisian dates seed is discarded yearly.

Methods

In this study, different solvents extraction (water, methanol, absolute acetone and aqueous acetone 80%) were used and the evaluation of its effect on phytochemical level, in vitro antioxidant activities, in vitro hyperglycemia key enzymes inhibition and in vivo anti-inflammatory proprieties were established for Tunisian date seeds.

Results

The result revealed that the polar solvent exhibited the highest amount of bioactive compounds. The correlation between polyphenol compounds and the antioxidant potentiality explains the powerful effect of used polar solvents on inflammation, TBARS and hyperglycemia inhibition. Furthermore, it showed its higher capacity to scavenge radicals.

Conclusions

Therefore, this big waste of Tunisian seeds could be used as cheap source of natural antioxidant compounds which are considered as a health challenge for the poor countries.
Appendix
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Literature
1.
Xu Y, Fan M, Ran J, Zhang T, Sun H, Dong M, Zhang Z, Zheng H. Variation in phenolic compounds and antioxidant activity in apple seeds of seven cultivars. Saudi J Biol Sci. 2016;23:379–88.CrossRefPubMed
2.
Al AKH A, Hasan MA. Antioxidant Activity in Some Citrus Leaves and Seeds Ethanolic Extracts. International Conference on Advances in Agricultural, Biological & Environmental Sciences. 2015:22–3.
3.
Habib H, Ibrahim W. Effect of date seeds on oxidative damage and antioxidant status in vivo. J Sci Food Agric. 2011;91:674–1679.CrossRef
4.
Maritime AC, Sanders RA, Watkins JB. Diabetes, oxidative stress, and antioxidants: a review. J Biochem Mol Toxicol. 2003;17:24–38.CrossRef
5.
Kumar S, Kumar V, Rana M, Kumar D. Enzymes inhibitors from plants: an alternate approach to treat diabetes. Pharmacogn Commun. 2012;2:18–33.CrossRef
6.
Bray GA, Greenway FL. Current and potential drugs for treatment of obesity. Endocr Rev. 1999;20:805–79.CrossRefPubMed
7.
Riaz M, Zia-Ul-Haq M, Saad B. Anthocyanins and Human Health: Biomolecular and Therapeutic Aspects. Springer International Publishing; 2016.
8.
Zia-Ul-Haq M, Riaz M, DeFeo V, Jaafar HZE, Moga M. Rubus fruticosus L.: constituents, biological activities and health related uses. Molecules. 2014;19(8):10998–1029.CrossRefPubMed
9.
10.
Halliwell B, Gutteridge JMC. Free radicals in Biology and Medicine 3rd ed Oxford: Oxford University Press. 1999;23–27.
11.
Talhouk R, Karam C, Fostok S, El-Jouni W, Barbour E. Anti-inflammatory bioactivities in plant extracts. J Med Food. 2007;10:1–10.CrossRefPubMed
12.
Mohamed DA, Al-Okbi SY. In vivo evaluation of antioxidant and anti inflammatory activity of different extracts of date fruits in adjuvant arthritis. Pol J Food Nutr Sci. 2004;13(54):397–402.
13.
Kehili HE, Zerizer S, Beladjila KA, Kabouche Z. Anti-inflammatory effect ofAlgerian date fruit(Phoenix dactylifera). Food Agric Immunol. 2016:820–9.
14.
Arzi A, Azarbani S, Aghel N, Nazari Z, Rezaei M. The preventive effect of date palm (Phoenix dactylifera) seed and fruit Hydroalcoholic extracts on carrageenan-induced inflammation in Male rat’s hind paw. Jundishapur Sci Med J. 2014;13(5):495–502.
15.
Mercken EM, Crosby SD, Lamming DW, JeBailey L, Krzysik-Walker S, Villareal DT. Calorie restriction in humans inhibits the PI3K/AKT pathway and induces a younger transcription profile. Aging Cell. 2013;12:645–51.CrossRefPubMedPubMedCentral
16.
Al-Farsi MA, Lee CY. Optimization of phenolics and dietary fibre extraction from date seeds. Food Chem. 2008;108:977–85.CrossRefPubMed
17.
Rababah TM, Banat F, Rababah A, Ereifej K, Yang W. Optimization of extraction conditions of total phenolics, antioxidant activities, and anthocyanin of oregano, thyme, terebinth, and pomegranate. J Food Sci. 2010;75(7):626–32.CrossRef
18.
Pellegrini N, Colombi B, Salvatore S, Brenna OV, Galaverna G, Del Rio D, Bianchi M, Bennett R, Brighenti F. Evaluation of antioxidant capacity of some fruit and vegetable foods: efficiency of extraction of a sequence of solvents. J Sci Food Agric. 2007;87:103–11.CrossRef
19.
Paras S, Hardeep SG. Antioxidant and phenol oxidase activity of germinated barley and its milling fractions. Food Chem. 2010;120:673–8.CrossRef
20.
Reis FS, Heleno SA, Barros L, Sousa MJ, Martins A, Santos-Buelga C, Ferreira ICFR. Toward the antioxidant and chemical characterization of mycorrhizal mushrooms from Northeast Portugal. J Food Sci. 2011;76:824–30.CrossRef
21.
El Arem A, Saafi EB, Mechri B, Lahouar L, Issaoui M, Hammami M, Achour L. Effects of the ripening stage on phenolic profile, phytochemical composition and antioxidant activity of date palm fruit. J Agric Food Chem. 2012;60:10896–902.CrossRef
22.
Dogra NK. Phytochemical analysis and in vitro antioxidant studies of Plumeria obtusa L. Leaves Indian J Pharm Sci. 2016;78(1):169–71.CrossRefPubMed
23.
Tao Y, Zhang Y, Cheng Y, Wang Y. Rapid screening and identification of α-glucosidase inhibitors from mulberry leaves using enzyme-immobilized magnetic beads coupled with HPLC/MS and NMR. Biomed Chromatogr. 2013:27(2):148–55.
24.
Rengasamy KR, Aderogba MA, Amoo SO, Stirk WA, Van Staden J. Potential antiradical and alpha-glucosidase inhibitors from Ecklonia maxima (Osbeck) Papenfuss. Food Chem. 2013;141(2):1412–5.CrossRefPubMed
25.
Deguchi Y, Osada K, Watanuki M. Effect of guava leaf extract in combination with acarbose or voglibose on increased blood glucose level in sugar-loaded normal mice. J Jpn Soc Nutr Food Sci. 2003;56:207–12.CrossRef
26.
Kilkenny C, Browne WJ, Cuthill IC, Emerson M, Altman DG. Improving bioscience research reporting: the ARRIVE guidelines for reporting animal research. PLoS Biol. 2010;8:e1000412.CrossRefPubMedPubMedCentral
27.
Winter CA, Risley EA, Nuss GW. Carrageenan-induced oedema in the hind paw of rat as an assay for anti-inflammatory activity. ProcSoc Exp Biol Ther. 1962;111:544–7.CrossRef
28.
Harborne JB. Phytochemical methods a guide to modern techniques of plant analysis. Plant Pathol J. 1999;48:146.CrossRef
29.
Liu X, Dong M, Chen X, Jiang M, Lv X, Yan G. Antioxidant activity and phenolics of an endophytic Xylaria sp. From Ginkgo biloba. Food Chem. 2007;105(2):548–54.CrossRef
30.
Razzaghi-Asl N, Garrido J, Khazraei H, Borges F, Firuzi O. Antioxidant properties of hydroxycinnamic acids: a review of structure-activity relationships. Curr Med Chem J. 2013;20(36):4436–50.CrossRef
31.
Zhou K, Yu L. Effects of extraction solvent on wheat bran antioxidant activity estimation. LWT-Food Sci Technol. 2004;37(7):717–21.CrossRef
32.
WHO. Global Strategy on Diet, Physical Activity and Health, Annual Report, Geneva, Switzerland. 2006;1–2.
33.
El-Kaissi S, Sherbeeni S. Pharmacological management of type 2 diabetes mellitus: an update. Curr Diabetes Rev. 2011;7(6):392–405.CrossRefPubMed
34.
Ranilla LG, Kwon YI, Apostolidis E, Shetty K. Phenolic compounds, antioxidant activity and in vitro inhibitory potential against key enzymes relevant for hyperglycemia and hypertension of commonly used medicinal plants, herbs and spices in Latin America. Bioresour Technol. 2010;101(12):4676–89.CrossRefPubMed
35.
Wild S, Roglic G, Green A, Sicree R, King H. Global prevalence of diabetes estimates for the year 2000 and projections for 2030. Diabetes Care. 2004;27:1047–53.CrossRefPubMed
36.
Fowler MJ. Diabetes treatment, part 2: oral agents for glycemic management. Clin Diabetes. 2007;25:131–4.CrossRef
37.
Valiathan MS. Healing plants. Curr Sci. 1998;75(11):1122–7.
38.
Sales PM, Souza PM, Simeoni LA, Silveira D. A-amylase inhibitors: a review of raw material and isolated compounds from plant source. J Pharm Pharm Sci. 2012;15:141–83.CrossRefPubMed
39.
Brahmachari G. Bio-flavonoids with promising antidiabetic potentials: a critical survey. In: Tiwari VK, Mishra BB, editors. Opportunity, challenge and scope of natural products in medicinal chemistry, Trivandrum: research signpost, vol. 2; 2011. p. 187–212.CrossRef
40.
Tundis R, Loizzo MR, Menichini F. Natural products as alpha-amylase and alpha-glucosidase inhibitors and their hypoglycemic potential in the treatment of diabetes: an update. Mini Rev Med Chem. 2010;10(4):315–31.CrossRefPubMed
41.
Rohn S, Rawel HM, Kroll J. Inhibitory effects of plant phenols on the activity of selected enzymes. J Agric Food Chem. 2002;50:3566–71.CrossRefPubMed
42.
Cazarolli LH, Zanatta L, Alberton EH, Figueiredo MS, Folador P, Damazio RG. Flavonoids: prospective drug candidates. Mini Rev Med Chem. 2008;8:1429–40.CrossRefPubMed
43.
Kim JS, Kwon CS, Son KH. Inhibition of alpha-glucosidase and amylase by luteolin, a flavonoid. Biosci Biotechnol Biochem. 2000;64:2458–61.CrossRefPubMed
44.
Khan SA, Al Kiyumi AR, Al Sheidi MS, Al Khusaibi TS, Al Shehhi NM, Alam T. In vitro inhibitory effects on a-glucosidase and a-amylase level and antioxidant potential of seeds of Phoenix dactylifera L. Asian Pac J Trop Biomed. 2016;6(4):322–9.CrossRef
45.
El-Far AH, Shaheen HM, Abdel-Daim MM, Al Jaouni SK, Mousa SA. Date palm (Phoenix dactylifera): protection and remedy food. Current Trends in Nutraceuticals. 2016;1:2–9.
46.
Sosa S, Balick MJ, Arvigo R, Esposito RG, Pizza C, Altinier G, Tubaro A. Screening of the topical antiinfl ammatory activity of some central American plants. J Ethnopharmacol. 2002;81:211–5.CrossRefPubMed
47.
Vinegar R, Truax JF, Selph JH, Johnsstone PR, Venable AL, Mckenzie KK. Pathway to carrageenan induced inflammation of the hind limb of the rat. Fed Proc. 1987;6:118–26.
48.
Antonio MA, Brito ARMS. Oral anti-inflammatory and anti-ulcerogenic activities of a hydroalcoholic extract and partitioned fractions of Turnera ulmifolia (Turneraceae). J Ethnopharmacol. 1998;61:215–28.CrossRefPubMed
49.
Lope ER, Chapadeiro E, Raso P, Tafuri WL. Bogliolo – Patologia. 4. ed. Belo Horizonte: Guanabara Koogan. 1987;67–112.
50.
Xiao J, Jiang X, Chen X. Antibacterial, anti-inflammatory and diuretic effect of flavonoids from Marchantia convoluta. Afr J Traditional Complementary Altern Med. 2005;2:244–52.
51.
Delaporte RH, Sanchez GM, Cuellar AC, Giuliani A, Palazzo de Mello JC. Anti-inflammatory activity and lipid peroxidation inhibition of iridoid lamiide isolated from Bouchea fluminensis (Vell.) Mold. (Verbenaceae). J Ethnopharmacol. 2002;82:127–30.CrossRefPubMed
52.
Geronikaki AA, Gavalas AM. Antioxidants and anti-inflammatory diseases: synthetic and natural antioxidants with anti-inflammatory activity. Combinatorial Chemistry & High Through put Screening. 2006;9:425–42.CrossRef
53.
Baliga MS, Baliga BRV, Kandathil SM, Bhat HP, Vayalil PK. A review of the chemistry and pharmacology of the date fruits (Phoenix dactylifera L.). Food Res Int. 2011;44:1812–22.CrossRef
Metadata
Title
Effect of solvents extraction on phytochemical components and biological activities of Tunisian date seeds (var. Korkobbi and Arechti)
Authors
Amira Thouri
Hassiba Chahdoura
Amira El Arem
Amel Omri Hichri
Rihab Ben Hassin
Lotfi Achour
Publication date
01-12-2017
Publisher
BioMed Central
Published in
BMC Complementary Medicine and Therapies / Issue 1/2017
Electronic ISSN: 2662-7671
DOI
https://doi.org/10.1186/s12906-017-1751-y

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