Skip to main content
Key Specialties
Cardiology Diabetology Endocrinology Gastroenterology Geriatrics and Gerontology Gynecology and Obstetrics Hematology Infectious Disease Internal Medicine Nephrology Neurology Oncology Pediatrics Respiratory Medicine Rheumatology Surgery
Congress Coverage
2024 ACC Congress 2023 ESMO Congress 2023 EASD Congress 2023 ERS Congress 2023 ESC Congress 2023 EHA Congress 2023 ASCO Annual Meeting
Clinical Collections
Advances in Alzheimer’s

Keynote webinar | Spotlight on sleep in brain health

LIVE: Thursday 2nd May 2024, 18:00-19:30 (CEST)

Quality sleep is essential for health. But what happens to our brains when sleep patterns are disturbed? Join our experts to explore the interplay between sleep disruption and neurological diseases, and the questions that you need to be asking your patients to help you prevent the harmful effects of sleep deprivation.
ADVANCED SEARCH
Log in
Top
BMC Complementary Medicine and Therapies
Published in: BMC Complementary Medicine and Therapies 1/2016

Open Access 01-12-2016 | Research article

Antibacterial and antibiotic-modulation activity of six Cameroonian medicinal plants against Gram-negative multi-drug resistant phenotypes

Authors: Doriane E. Djeussi, Jaurès A. K. Noumedem, Bonaventure T. Ngadjui, Victor Kuete

Published in: BMC Complementary Medicine and Therapies | Issue 1/2016

Login to get access

Abstract

Background

Bacterial Infections involving multi-drug resistant (MDR) phenotypes constitute a worldwide health concern. The present work was designed to assess the antibacterial properties of the methanol extracts of six medicinal plants (Anthocleista schweinfurthii, Nauclea latifolia, Boehmeria platyphylla, Caucalis melanantha, Erigeron floribundus and Zehneria scobra) and the effects of their associations with antibiotics on MDR Gram-negative bacteria over-expressing active efflux pumps.

Methods

The antibacterial activities and the ability to potentiate antibiotic effects of the methanol extracts the tested plants were evaluated in vitro against twenty eight Gram-negative bacteria expressing MDR phenotypes, using broth microdilution method. The phytochemical screening of these extracts was also performed using standard methods.

Results

All tested extracts displayed moderate to low antibacterial activity on at least 14.3 % of the 28 tested bacteria, with MIC values ranged from 128 to 1024 μg/mL. The best antibacterial spectrum was observed with Naulcea latifolia bark extract. Extracts from A. schweinfurthii fruits, N. latifolia stem bark, Z. scobra and N. latifolia leaves showed synergistic effects with many antibiotics against MDR bacteria.

Conclusion

The overall results of the present study provide information for the possible use of the studied plants, especially Nauclea latifolia in the control of Gram-negative bacterial infections including MDR species as antibacterials as well as resistance modulators.
Appendix
Available only for authorised users
Literature
1.
2.
Targant H. L’îlot de multirésistance aux antibiotiques, Salmonella Genomic Island 1 (SGI1): variabilité, diffusion inter - espèces et implication dans la virulence. Lyon: Université Claude Bernard de Lyon 1; 2010.
3.
Poole K. Efflux-mediated multiresistance in Gram-negative bacteria. Clin Microbiol Infect. 2004;10:12–26.CrossRefPubMed
4.
Kuete V. Potential of Cameroonian plants and derived products against microbial infections: a review. Planta Med. 2010;76:1479–91.CrossRefPubMed
5.
Noumedem J, Mihasan M, Kuiate J, Stefan M, Cojocaru D, Dzoyem J, Kuete V. In vitro antibacterial and antibiotic-potentiation activities of four edible plants against multidrug-resistant Gram-negative species. BMC Complement Altern Med. 2013; 13:190.
6.
Lacmata ST, Kuete V, Dzoyem JP, Tankeo SB, Teke GN, Kuiate JR, Pages J-M. Antibacterial activities of selected Cameroonian plants and their synergistic effects with antibiotics against bacteria expressing MDR phenotypes. Evid Based Complement Alternat Med. 2012;2012:623723.CrossRefPubMedPubMedCentral
7.
Nelson ML, Levy SB. Reversal of tetracycline resistance mediated by different bacterial tetracycline resistance determinants by an inhibitor of the Tet(B) antiport protein. Antimicrob Agents Chemother. 1999;43:1719–24.PubMedPubMedCentral
8.
German N, Wei P, Kaatz GW, Kerns RJ. Synthesis and evaluation of fluoroquinolone derivatives as substrate-based inhibitors of bacterial efflux pumps. Eur J Med Chem. 2008;43:2453–63.CrossRefPubMed
9.
Harbone JB. Phytochemical methods: A guide to modern techniques of plant analysis. 1973.
10.
Harbone J (ed.). Phytochemical methods: a guide to modern techniques of plant analysis. London: Chapman & Hall; 1973.
11.
Ngameni B, Fotso GW, Kamga J, Ambassa P, Abdou T, Fankam AG, Voukeng IK, Ngadjui BT, Abegaz BM, Kuete V. 9 - Flavonoids and Related Compounds from the Medicinal Plants of Africa. In: Kuete V, editor. Medicinal Plant Research in Africa. Oxford: Elsevier; 2013. p. 301–50.CrossRef
12.
Wansi JD, Devkota KP, Tshikalange E, Kuete V. 14 - Alkaloids from the Medicinal Plants of Africa. In: Kuete V, editor. Medicinal Plant Research in Africa. Oxford: Elsevier; 2013. p. 557–605.CrossRef
13.
Poumale HMP, Hamm R, Zang Y, Shiono Y, Kuete V. 8 - Coumarins and Related Compounds from the Medicinal Plants of Africa. In: Kuete V, editor. Medicinal Plant Research in Africa. Oxford: Elsevier; 2013. p. 261–300.CrossRef
14.
Eloff JN. A sensitive and quick microplate method to determine the minimal inhibitory concentration of plant extracts for bacteria. Planta Med. 1998;64:711–3.CrossRefPubMed
15.
Coutinho HD, Lima JG, Siqueira-Júnior JP. Additive effects of Hyptis martiusii Benth with aminoglycosides against Escherichia coli. Indian J Med Res. 2010;131:106–8.PubMed
16.
17.
Kuete V. Medicinal Plant Research in Africa. In: Kuete V, editor. Pharmacology and Chemistry. 2013. p. Oxford–Elsevier.
18.
Tran QT, Mahendran KR, Hajjar E, Ceccarelli M, Davin-Regli A, Winterhalter M, Weingart H, Pages JM. Implication of porins in beta-lactam resistance of Providencia stuartii. J Biol Chem. 2010;285:32273–81.CrossRefPubMedPubMedCentral
19.
Kuete V, Ngameni B, Tangmouo JG, Bolla JM, Alibert-Franco S, Ngadjui BT, Pages JM. Efflux pumps are involved in the defense of Gram-negative bacteria against the natural products isobavachalcone and diospyrone. Antimicrob Agents Chemother. 2010;54:1749–52.CrossRefPubMedPubMedCentral
20.
Kuete V, Alibert-Franco S, Eyong KO, Ngameni B, Folefoc GN, Nguemeving JR, Tangmouo JG, Fotso GW, Komguem J, Ouahouo BM, Bolla JM, Chevalier J, Ngadjui BT, Nkengfack AE, Pagès JM. Antibacterial activity of some natural products against bacteria expressing a multidrug-resistant phenotype. Int J Antimicrob Agents. 2011;37:156–61.CrossRefPubMed
21.
Ngbolua K-t-N, Mubindukila REN, Mpiana PT, Ashande MC, Baholy R, Ekutsu GE, Gbolo ZB, Fatiany PR. In vitro assessment of antibacterial and antioxidant activities of a Congolese medicinal plant species Anthocleista schweinfurthii Gilg (Gentianaceae). J Modern Drug Discov Drug Deliv Res. 2014;3:1-6.
22.
Mbaveng AT, Hamm R, Kuete V. 19 - Harmful and Protective Effects of Terpenoids from African Medicinal Plants. In: Kuete V, editor. Toxicological Survey of African Medicinal Plants. Oxford: Elsevier; 2014. p. 557-576.
23.
24.
Bruneton J. Toxic plants. Dangerous to humans and animals. Paris: Tec & Doc; 1999.
25.
Al-Shamma A, Drake SD, Guagliardi LE, Mitscher LA, Swayze JK. Antimicrobial alkaloids from Boehmeria cylindrica. Phytochemistry. 1982;21:485–7.CrossRef
26.
Choudhary AN, Juyal V. Synthesis of chalcone and their derivatives as antimicrobial agents. Int J Pharm Pharmaceut Sci. 2011;3:125–8.
27.
Hart N, Johns S, Lamberton J. 3,4-Dimethoxy-ω-(2'-piperidyl)acetophenone, a new alkaloid from Boehmeria platyphylla Don. (family Urticaceae). Aust J Chem. 1968;21:1397–8.CrossRef
28.
Bhattarai KR, Maren IE, Chaudhary RP. Medicinal plant knowledge of the Panchase region in the middle hills of the Nepalese Himalayas. Banko Janakari. 2011;21(2):31–9.
29.
Kuiate J, Kuate S, Kemadjou N, Djokoua S, Zifack F, Foko J. Antidermatophitic activities of nine (9) essential oils. East Centr Afr J Pharmaceut Sci. 2006;7:6–9.
30.
Kuiate J-R, Tsona AA, Foko J, Bessiere JM, Menut C, Zollo P-HA. Chemical composition and in vitro antifungal properties of essential oils from leaves and flowers of Erigeron floribundus (H.B. et K.) Sch. Bip. From Cameroon. J Essent Oil Res. 2005;17:261–4.CrossRef
31.
Tchoumbougnang F, Jazet DPM, Wouatsa NAV, Fekam BF, Sameza ML, Amvam ZPH, Menut C. Composition and antifungal properties of essential oils from five plants growing in the mountainous area of the West Cameroon. J Essent Oil Bear Plants. 2013;16:679–88.CrossRef
32.
Bi FT, Koné M, Kouamé N. Antifungal activity of Erigeron floribundus (Asteraceae) from Cote d´Ivoire, West Africa. Trop J Pharmaceut Res. 2008;7:975–9.
33.
Akoachere J-FTK, Suylika Y, Mbah AJ, Ayimele AG, Assob JCN, Chegaing Fodouop SP, Kodjio N, Gatsing D. In vitro antimicrobial activity of agents from Spilanthes filicaulis and Laportea ovalifolia against some drug resistant bacteria. Br J Pharmaceut Res. 2015;6:76–87.CrossRef
34.
Okwulehie IC, Akanwa FE. Antimicrobial activity of ethanol extract of four indigenous plants from South Eastern Nigeria. ARPN J Sci Technol. 2013;3:350–5.
35.
Mbouangouere R, Tane P, Ngamga D, Khan S, Choudhary M, Ngadjui B. A New Steroid and Î-glucosidase Inhibitors from Anthocleista schweinfurthii. Res J Med Plant. 2007;1:106.CrossRef
36.
Rajbhandari KR. Ethnobotany of Nepal, 1st edition edn. Kathmandu: Ethnobotanical Society of Nepal; 2001.
37.
Narayan EM. Plants and people of Nepal. Narayan E Manandhar. Portland: Timber Press, Oregon; 2002.
38.
Kidane B, Andel T, Maesen LJG, Asfaw Z. Use and management of traditional medicinal plants by male and Ari ethnic communities in southern Ethiopia. J Ethnobiol Ethnomed. 2014;10:46.CrossRefPubMedPubMedCentral
39.
Yineger H, Kelbess E, Bekel T, Luleka E. Ethnoveterinary medicinal plants at Bale Mountains National Park, Ethiopia. J Ethnopharmacol. 2007;112:55–70.CrossRefPubMed
40.
Focho DA, Ndam WT, Fonge BA. Medicinal plants of Aguambu – Bamumbu in the Lebialem highlands, southwest province of Cameroon. Afr J Pharm Pharmacol. 2009;3(1):1–13.
41.
Yapo F, Yapi F, Ahiboh H, Hauhouot-Attounbre M-L, Guédé N, Djaman J, Monnet D. Immunomodulatory effect of the aqueous extract of Erigeron floribundus (Kunth) Sch Beep (Asteraceae) Leaf in Rabbits. Trop J Pharmaceut Res. 2011;10:187–93.
42.
Asongalem E, Foyet H, Ngogang J, Folefoc G, Dimo T, Kamtchouing P. Analgesic and antiinflammatory activities of Erigeron floribundus. J Ethnopharmacol. 2004;91:301–8.CrossRefPubMed
43.
Berto C, Maggi F, Nya P, Pettena A, Boschiero I, Dall'Acqua S. Phenolic constituents of Erigeron floribundus (Asteraceae), a Cameroonian medicinal plant. Nat Prod Commun. 2014;9:1691–4.PubMed
44.
Tra Bi F, Koné M, Kouamé N. Antifungal activity of Erigeron floribundus (Asteraceae) from Côte d’Ivoire, West Africa. Trop J Pharmaceut Res. 2008;7:975–9.
45.
Abbiw DK. Useful plants of Ghana: West African uses of wild and cultivated plants. London: Intermediate Technology Publications; 1990.
46.
Akabue P, Mittal H. Clinical evaluation of a traditional herbal practice in Nigeria: A preliminary report. J Ethnopharmacol. 1982;6:355–9.CrossRef
47.
Madubunyi I. Anti- hepatotoxic and trypanocidal activities of the ethanolic extract of Nauclea latifolia root bark. J Herbs Spices Med Plants. 1995;3:23–53.CrossRef
48.
Elujoba A. Female infertility in the hands of traditional birth attendants in South-West Nigeria. Fitoterapia. 1995;66:239–48.
49.
Anowi CF, Nnabuife CC, Mbah C, Onyekaba T. Antimicrobial properties of the methanolic extract of the leaves of Nauclea latifolia. Int J Drug Res Technol. 2012;2:45–55.
50.
Shigemori H, Kagata T, Ishiyama H, Morah F, Ohsaki A, Kobayashi J. Naucleamides A-E: new monoterpene indole alkaloids from Nauclea latifolia. Chem Pharmaceut Bull. 2003;51:58–61.CrossRef
51.
Tegenu G. Antimicrobial activity of solvent extracts of Cucumis ficifolius and Zehneria scabra on test microorganisms. Ethiopia: Addis Ababa University; 2011.
52.
Arulappan MT, Britto JS, Ruckmani K, Kumar MR. Antimicrobial and antifungal activities of Zehneria scabra (L.F.) sond against human pathogens. Int J Develop Res. 2015;5:3852–9.
Metadata
Title
Antibacterial and antibiotic-modulation activity of six Cameroonian medicinal plants against Gram-negative multi-drug resistant phenotypes
Authors
Doriane E. Djeussi
Jaurès A. K. Noumedem
Bonaventure T. Ngadjui
Victor Kuete
Publication date
01-12-2016
Publisher
BioMed Central
Published in
BMC Complementary Medicine and Therapies / Issue 1/2016
Electronic ISSN: 2662-7671
DOI
https://doi.org/10.1186/s12906-016-1105-1

Other articles of this Issue 1/2016

BMC Complementary Medicine and Therapies 1/2016 Go to the issue

Research article

Methanolic extract of Agerantum conyzoides exhibited toxicity and growth disruption activities against Anopheles gambiae sensu stricto and Anopheles arabiensis larvae

Research article

Effects of phenolic-rich extracts of Clinacanthus nutans on high fat and high cholesterol diet-induced insulin resistance

Research article

Bo-Gan-Whan regulates proliferation and migration of vascular smooth muscle cells

Research article

Evaluation of the anti-diarrheal activity of the leaf extract of Croton macrostachyus Hocsht. ex Del. (Euphorbiaceae) in mice model

Research article

Danshen enhanced the estrogenic effects of Qing E formula in ovariectomized rats

Research article

Paeoniflorin inhibits excitatory amino acid agonist-and high-dose morphine-induced nociceptive behavior in mice via modulation of N-methyl-D-aspartate receptors