Skip to main content
Key Specialties
Cardiology Diabetology Endocrinology Gastroenterology Geriatrics and Gerontology Gynecology and Obstetrics Hematology Infectious Disease Internal Medicine Nephrology Neurology Oncology Pediatrics Respiratory Medicine Rheumatology Surgery
Congress Coverage
2024 ACC Congress 2023 ESMO Congress 2023 EASD Congress 2023 ERS Congress 2023 ESC Congress 2023 EHA Congress 2023 ASCO Annual Meeting
Clinical Collections
Advances in Alzheimer’s

Keynote webinar | Spotlight on sleep in brain health

LIVE: Thursday 2nd May 2024, 18:00-19:30 (CEST)

Quality sleep is essential for health. But what happens to our brains when sleep patterns are disturbed? Join our experts to explore the interplay between sleep disruption and neurological diseases, and the questions that you need to be asking your patients to help you prevent the harmful effects of sleep deprivation.
ADVANCED SEARCH
Log in
Top
BMC Complementary Medicine and Therapies
Published in: BMC Complementary Medicine and Therapies 1/2016

Open Access 01-12-2015 | Research article

Assessment of cytotoxicity and genotoxicity of stem bark extracts from Canarium odontophyllum Miq. (dabai) against HCT 116 human colorectal cancer cell line

Authors: Dayang Fredalina Basri, Zafira Ayushah Zainul Alamin, Kok Meng Chan

Published in: BMC Complementary Medicine and Therapies | Issue 1/2016

Login to get access

Abstract

Background

Canarium odontophyllum Miq. is a plant species widely known as ‘dabai’ and can be vastly found in Sarawak. The aim of this study was to assess the cytotoxic and genotoxic effect of extracts from stem bark of C. odontophyllum against HCT 116 human colorectal cancer cell line.

Method

The IC50 values of the aqueous, methanol, and acetone extracts against HCT 116 cells as well as the acetone extract against human colon fibroblast cell line CCD-18co were determined using the MTT assay. The concentration of the extracts ranged from 12.5 to 200 μg/ml at treatment time of 24, 48 and 72 h. Annexin V-FITC/PI labelling assay was employed to determine mode of HCT 116 cell death induced by acetone extract at 48 h. The DNA damage induced by the extract in HCT 116 cells was detected using alkaline comet assay at 30 min of IC10 and IC25 treatment.

Results

Acetone extract exhibited the highest cytotoxic effect against HCT 116 cells compared to methanol and aqueous extract at 24, 48 and 72 h. Despite no cytotoxic effect by acetone extract against CCD-18co cells at 24 and 48 h, however at 72 h, CCD-18co cells proliferated. Apoptosis assessment using Annexin V-FITC/PI labelling assay revealed that the primary cell death was via apoptosis after 48 h treatment. Low doses of acetone extract from stem bark of C. odontophyllum showed significant DNA damage in HCT 116 cells with tail moment of 6.187 ± 0.718 A.U and 7.877 ± 0.142 A.U, respectively.

Conclusions

Acetone extract from stem bark of C. odontophyllum has high potential in the development of anticancer agent against HCT 116 cells with no cytotoxic effect against human colon fibroblast cells.
Literature
1.
Underwood JCE, Cross SS. General and Systemic Pathology. Churchill Livingstone: Elsevier; 2009. p. 395.
2.
Cancer Genome Atlas Network. Comprehensive molecular characterization of human colon and rectal cancer. Nature. 2012;487(7407):330–7.CrossRef
3.
4.
Creţu E, Trifan A, Vasincu A, Miron A. Plant-derived anticancer agents-curcumin in cancer prevention and treatment. Rev Med Chir Soc Med Nat lasi. 2012;116(4):1223–9.
5.
Ahmed M, Khan MI, Khan MR, Muhammad N, Khan AU. Role of medicinal plants in oxidative stress and cancer. Open Access Sci Rep. 2013;2(2):641.
6.
Azlan A, Nasir NNM, Amom Z, Ismail A. Physical properties of skin, flesh, and kernel of Canarium odontophyllum fruit. J Food Agric Environ. 2009;7:55–7.
7.
Chua HP, Nicholas D. Dabai - Speciality fruit of Sarawak. Agromedia. 2009;30:28–30.
8.
Chew LY, Khoo HE, Amin I, Azrina A, Lau CY. Analysis of phenolic compounds of dabai (Canarium odontophyllum Miq.) fruits by high-performance liquid chromatography. Food Anal Method. 2012;5(1):126–37.CrossRef
9.
Basri DF, Saidi N, Mahari H, Saari S, Santhanam J. Preliminary screening for antimicrobial activity of the pulp of C. odontophyllum Miq. (Dabai) fruit. Global J Pharmacol. 2014;8(2):213–20.
10.
Basri DF, Ishak SF, Zin NM. Shell extract of seed from Canarium odontophyllum Miq. (dabai) fruit as potential source of antibacterial agent. Int J Pharm Sci Rev Res. 2014;28(2):257–62.
11.
Basri DF, Shabry ASM, Meng CK. Leaves extract from Canarium odontophyllum Miq. (dabai) exhibits cytotoxic activity against human colorectal cancer cell HCT 116. Nat Prod Chem Res. 2015;3(2):1–4.
12.
Basri DF, Mohd MAAR, Meng CK, Latif ES, Huyop FZ. Cytotoxic Activity of Stem Bark Extracts from Canarium odontophyllum Miq. (Dabai) against Human Colorectal Carcinoma HCT 116 Cell Line. Am J Plant Sci. 2014;5:3925–33.CrossRef
13.
Wouters MD, Van Gent DC, Hoeijmakers JH, Pothof J. MicroRNAs, the DNA damage response and cancer. Mutat Res. 2011;717(1):54–66.PubMedCrossRef
14.
Roos WP, Kaina B. DNA damage-induced cell death: from specific DNA lesions to the DNA damage response and apoptosis. Cancer Lett. 2013;332(2):237–48.PubMedCrossRef
15.
Liao W, McNutt MA, Zhu WG. The comet assay: a sensitive method for detecting DNA damage in individual cells. Methods. 2009;48(1):46–53.PubMedCrossRef
16.
Mosmann T. Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J Immunol Methods. 1983;65(1):55–63.PubMedCrossRef
17.
Vermes I, Haanen C, Steffens-Nakken H, Reutellingsperger C. A novel assay for apoptosis flow cytometric detection of phosphatidylserine expression on early apoptotic cells using fluorescein labelled Annexin V. J Immunol Methods. 1995;184(1):39–51.PubMedCrossRef
18.
Singh NP, McCoy MT, Tice RR, Schneider EL. A simple technique for quantitation of low levels of DNA damage in individual cells. Exp Cell Res. 1988;175(1):184–91.PubMedCrossRef
19.
Ali R, Mirza Z, Ashraf GM, Kamal MA, Ansari SA, Damanhouri GA, et al. New anticancer agents: recent developments in tumor therapy. Anticancer Res. 2012;32(7):2999–3005.PubMed
20.
Centoducati G, Santacroce MP, Lestingi A, Casalino E, Crescenzo G. Characterization of the cellular damage induced by aflatoxin B1 in sea bream (Sparus aurata Linnaeus, 1758) hepatocytes. Ital J Anim Sci. 2010;8 Suppl 2:848–50.CrossRef
21.
Bouayed J, Bohn T. Exogenous antioxidants-Double-edged swords in cellular redox state. Health beneficial effects at physiologic doses versus deleterious effects at high doses. Oxid Med Cell Longev. 2010;3(4):228–37.PubMedPubMedCentralCrossRef
22.
Kashani LMT, Majdzadeh M, Khanavi M, Taghizadeh M, Sadati N, Kahkeshani N, et al. Cytotoxic activity of selected Iranian traditional medicinal plants on colon, colorectal and breast cancer cell lines. Archives of Breast Cancer. 2014;1(3):19–22.
23.
Lu JJ, Dang YY, Huang M, Xu WS, Chen XP, Wang YT. Anti-cancer properties of terpenoids isolated from Rhizoma Curcumae-A review. J Ethnopharmacol. 2012;143(2):406–11.PubMedCrossRef
24.
Kanadaswami C, Lee LT, Lee PPH, Hwang JJ, Ke FC, Huang YT, et al. The antitumor activities of flavonoids. In vivo. 2005;19(5):895–909.PubMed
25.
Taraphdar AK, Roy M, Bhattacharya RK. Natural products as inducers of apoptosis: Implication for cancer therapy and prevention. Curr Sci. 2001;80(11):1387–96.
26.
Li L, Adams LS, Chen S, Killian C, Ahmed A, Seeram NP. Eugenia jambolana Lam. berry extract inhibits growth and induces apoptosis of human breast cancer but not non-tumorigenic breast cells. J Agric Food Chem. 2009;57(3):826–31.PubMedPubMedCentralCrossRef
27.
Ohyama K, Akaike T, Hirobe C, Yamakawa T. Cytotoxicity and apoptotic inducibility of Vitex agnus-castus fruit extract in cultured human normal and cancer cells and effect on growth. Biol Pharm Bull. 2003;26(1):10–8.PubMedCrossRef
28.
Fesik SW. Promoting apoptosis as a strategy for cancer drug discovery. Nat Rev Cancer. 2005;5(11):876–85.PubMedCrossRef
29.
Ahmad NH, Rahim RA, Mat I. Catharanthus roseus aqueous extract is cytotoxic to Jurkat leukaemic T-cells but induces the proliferation of normal peripheral blood mononuclear cells. Trop Life Sci Res. 2010;21(2):101–13.PubMedPubMedCentral
30.
Stone SC, Vasconcellos FA, Lenardão EJ, do Amaral RC, Jacob RG, Leite FL. Evaluation of potential use of Cymbopogon sp. essential oils, (R)-citronellal and N-citronellylamine in cancer chemotherapy. Int J Appl Res Nat Prod. 2013;6(4):11–5.
31.
Hadi SM, Asad SF, Singh S, Ahmad A. Putative mechanism for anticancer and apoptosis-inducing properties of plant-derived polyphenolic compounds. IUBMB Life. 2000;50:167–71.PubMedCrossRef
32.
Moirangthem DS, Talukdar NC, Kasoju N, Bora U. Antioxidant, antibacterial, cytotoxic, and apoptotic activity of stem bark extracts of Cephalotaxus griffithii Hook. f. BMC Complement Altern Med. 2012;12:30.PubMedPubMedCentralCrossRef
33.
Seo WG, Pae HO, Oh GS, Chai KY, Yun YG, Chung HT, et al. Ethyl acetate extract of the stem bark of Cudrania tricuspidata induces apoptosis in human leukemia HL-60 cells. Am J Chin Med. 2001;29(2):313–20.PubMedCrossRef
34.
Nishibori N, Itoh M, Kashiwagi M, Arimochi H, Morita K. In vitro cytotoxic effect of ethanol extract prepared from sporophyll of Undaria pinnatifida on human colorectal cancer cells. Phytother Res. 2012;26(2):191–6.PubMedCrossRef
35.
36.
Ma X, Wang Z. Anticancer drug discovery in the future: an evolutionary perspective. Drug Discov Today. 2009;14(23):1136–42.PubMedCrossRef
37.
38.
Mesner PW, Budihardjo II, Kaufmann SH. Chemotherapy-induced apoptosis. Adv Pharmacol. 1997;41:461–99.PubMedCrossRef
39.
Chan KM, Rajab NF, Ishak MH, Ali AM, Yusoff K, Din LB, et al. Goniothalamin induces apoptosis in vascular smooth muscle cells. Chem Biol Interact. 2010;159:129–40.CrossRef
40.
Hartmann A, Agurell E, Beevers C, Brendler-Schwaab S, Burlinson B, Clay P, et al. Recommendations for conducting the in vivo alkaline Comet assay. Mutagenesis. 2003;18(1):45–51.PubMedCrossRef
41.
Chauvel-Lebret DJ, Auroy P, Tricot-Doleux S, Bonnaure-Mallet M. Evaluation of the capacity of the SCGE assay to assess the genotoxicity of biomaterials. Biomaterials. 2001;22(13):1795–801.PubMedCrossRef
42.
Olive PL, Banáth JP. The comet assay: a method to measure DNA damage in individual cells. Nat Protoc. 2006;1(1):23–9.PubMedCrossRef
43.
Swift LH, Golsteyn RM. Genotoxic anti-cancer agents and their relationship to DNA damage, mitosis, and checkpoint adaptation in proliferating cancer cells. Int J Mol Sci. 2014;15(3):3403–31.PubMedPubMedCentralCrossRef
44.
45.
Chorawala MR, Oza PM, Shah GB. Mechanisms of anticancer drugs resistance: an overview. Int J Pharm Sci Drug Res. 2012;4(1):1–9.
Metadata
Title
Assessment of cytotoxicity and genotoxicity of stem bark extracts from Canarium odontophyllum Miq. (dabai) against HCT 116 human colorectal cancer cell line
Authors
Dayang Fredalina Basri
Zafira Ayushah Zainul Alamin
Kok Meng Chan
Publication date
01-12-2015
Publisher
BioMed Central
Published in
BMC Complementary Medicine and Therapies / Issue 1/2016
Electronic ISSN: 2662-7671
DOI
https://doi.org/10.1186/s12906-016-1015-2

Other articles of this Issue 1/2016

BMC Complementary Medicine and Therapies 1/2016 Go to the issue

Research article

Intestinal and vascular smooth muscle relaxant effect of Viscum album explains its medicinal use in hyperactive gut disorders and hypertension

Research article

Photoprotective potential in some medicinal plants used to treat skin diseases in Sri Lanka

Research article

Antiosteoporotic effect of Petroselinum crispum, Ocimum basilicum and Cichorium intybus L. in glucocorticoid-induced osteoporosis in rats

Research article

Aqueous extract of Lithospermi radix attenuates oxaliplatin-induced neurotoxicity in both in vitro and in vivo models

Research article

Use of complementary and alternative medicine in patients with health complaints attributed to former dental amalgam fillings

Research article

Herbal remedies and functional foods used by cancer patients attending specialty oncology clinics in Trinidad