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BMC Complementary Medicine and Therapies
Published in: BMC Complementary Medicine and Therapies 1/2012

Open Access 01-12-2012 | Research article

Antioxidant, antibacterial, cytotoxic, and apoptotic activity of stem bark extracts of Cephalotaxus griffithii Hook. f

Authors: Dinesh Singh Moirangthem, Narayan Chandra Talukdar, Naresh Kasoju, Utpal Bora

Published in: BMC Complementary Medicine and Therapies | Issue 1/2012

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Abstract

Background

Cephalotaxus spp. are known to possess various therapeutic potentials. Cephalotaxus griffithii, however, has not been evaluated for its biological potential. The reason may be the remoteness and inaccessibility of the habitat where it is distributed. The main aim of this study was to: (1) evaluate multiple biological potentials of stem bark of C. griffithii, and (2) identify solvent extract of stem bark of C. griffithii to find the one with the highest specific biological activity.

Methods

Dried powder of stem bark of C. griffithii was exhaustively extracted serially by soaking in petroleum ether, acetone and methanol to fractionate the chemical constituents into individual fractions or extracts. The extracts were tested for total phenolic and flavonoid content, antioxidant (DPPH radical scavenging, superoxide radical scavenging, and reducing power models), antibacterial (disc diffusion assay on six bacterial strains), cytotoxic (MTT assay on HeLa cells), and apoptotic activity (fluorescence microscopy, DNA fragmentation assay, and flow cytometry on HeLa cells).

Results

Among the three extracts of stem bark of C. griffithii, the acetone extract contained the highest amount of total phenolics and flavonoids and showed maximum antioxidant, antibacterial, cytotoxic (IC50 of 35.5 ± 0.6 μg/ml; P < 0.05), and apoptotic (46.3 ± 3.6% sub-G0/G1 population; P < 0.05) activity, followed by the methanol and petroleum ether extracts. However, there was no significant difference observed in IC50 values (DPPH scavenging assay) of the acetone and methanol extracts and the positive control (ascorbic acid). In contrast, superoxide radical scavenging assay-based antioxidant activity (IC50) of the acetone and methanol extracts was significantly lower than the positive control (P < 0.05). Correlation analysis suggested that phenolic and flavonoid content present in stem bark of C. griffithii extracts was responsible for the high antioxidant, cytotoxic, and apoptotic activity (P < 0.05).

Conclusions

Stem bark of C. griffithii has multiple biological effects. These results call for further chemical characterization of acetone extract of stem bark of C. griffithii for specific bioactivity.
Appendix
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Literature
1.
Uma S: Blooming potential of the detached shoots of Cephalotaxus griffithii Hook. f. Curr Sci. 2008, 95: 320-321.
2.
Kuo Y-H, Lin C-H, Hwang S-Y, Shen Y-C, Lee Y-C, Li S-Y: Novel cytotoxic C-methylated biflavone from the stem of Cephalotaxus wilsoniana. Chem Pharm Bull. 2000, 48: 440-441. 10.1248/cpb.48.440.CrossRefPubMed
3.
Lee MK, Lin SW, Yang H, Sung HS, Lee H-S, Park MJ, Kim YC: Osteoblast differentiation stimulating activity of biflavonoids from Cephalotaxus koreana. Bioorg Medicinal Chem Lett. 2006, 16: 2850-2854. 10.1016/j.bmcl.2006.03.018.CrossRef
4.
Bae K, Jin W, Thoung PT, Min BS, Na M, Lee YM, Kang SS: A new flavonoid glycoside from the leaf of Cephalotaxus koreana. Fitoterapia. 2007, 78: 409-413. 10.1016/j.fitote.2007.02.008.CrossRefPubMed
5.
Kamil M, Khan NA, Ahmad I, Iiyas M, Rahman W: Chemical constituents of Cephalotaxus griffithii. J Indian Chem Soc. 1982, 59: 1199-
6.
Phutdhawong W, Korth J, Budhasukh D, Pyne SG: Volatile components from Cephalotaxus griffithii growing in northern Thailand. Flavour Fragr J. 2002, 17: 153-155. 10.1002/ffj.1064.CrossRef
7.
Plouvier V: Cephalotaxoside, a new apigenin heteroside isolated from Cephalotaxus. Presence of rhoifolin in Exochorda and Lupinus. C.r.Acad.sci, Ser.D: Sciences Naturelles. 1966, 263: 1529-1532.
8.
Wang L-W, Su H-J, Yang S-Z, Won S-J, Lin C-N: New alkaloids and a tetraflavonoid from Cephalotaxus wilsoniana. J Nat Prod. 2004, 67: 1182-1185. 10.1021/np0498657.CrossRefPubMed
9.
Gamet-Payrastre L, Manenti S, Gratacap MP, Tulliez J, Chap H, Payrastre B: Flavonoids and the inhibition of PKC and PI 3-kinase. Gen Pharmacol. 1999, 32: 279-286. 10.1016/S0306-3623(98)00220-1.CrossRefPubMed
10.
Middleton E, Kandaswami C, Theoharides TC: The effects of plant flavonoids on mammalian cells: implications for inflammation, heart disease and cancer. Pharmacol Rev. 2000, 52: 673-751.PubMed
11.
Nichols JA, Katiyar SK: Skin photoprotection by natural polyphenols: anti-inflammatory, antioxidant and DNA repair mechanisms. Arch Dermatol Res. 2010, 302: 71-83. 10.1007/s00403-009-1001-3.CrossRefPubMed
12.
Yuan YV, Walsh NA: Antioxidant and antiproliferative activities of extracts from a variety of edible seaweeds. Food Chem Toxicol. 2006, 44: 1144-1150. 10.1016/j.fct.2006.02.002.CrossRefPubMed
13.
Rao ASVC, Reddy SG, Babu PP, Reddy AR: The antioxidant and antiproliferative activities of methanolic extracts from Njavara rice bran. BMC Complem Altern Med. 2010, 10: 4-10.1186/1472-6882-10-4.CrossRef
14.
Carvalho M, Ferreira PJ, Mendes VS, Silva R, Pereira JA, Jerónimo C, Silva BM: Human cancer cell antiproliferative and antioxidant activities of Juglans regia L. Food Chem Toxicol. 2010, 48: 441-447. 10.1016/j.fct.2009.10.043.CrossRefPubMed
15.
Kook D, Wolf AH, Yu AL, Neubauer AS, Priglinger SG, Kampik A, Welge-Lüssen UC: The protective effect of quercetin against oxidative stress in the human RPE in vitro. Invest Ophthalmol Vis Sci. 2008, 49: 1712-1720. 10.1167/iovs.07-0477.CrossRefPubMed
16.
Nabavi SF, Eslami S, Moghaddam AH, Nabavi SM: Protective effects of curcumin against fluoride-induced oxidative stress in the rat brain. Neurophysiology. 2011, 43: 287-291. 10.1007/s11062-011-9228-y.CrossRef
17.
Nabavi SF, Moghaddam AH, Eslami S, Nabavi SM: Protective effects of curcumin against sodium fluoride-induced toxicity in rat kidneys. Biol Trace Elem Res. 2012, 145: 369-374. 10.1007/s12011-011-9194-7.CrossRefPubMed
18.
Shon M-Y, Kim T-H, Sung N-J: Antioxidants and free radical scavenging activity of Phellinus baumii (Phellinus of Hymenochaetoceae) extracts. Food Chem. 2003, 82: 593-597. 10.1016/S0308-8146(03)00015-3.CrossRef
19.
Scalbert A: Antimicrobial properties of tannins. Phytochemistry. 1991, 30: 3875-3883. 10.1016/0031-9422(91)83426-L.CrossRef
20.
21.
Cho CH, Yoo G, Kim S, Lee G-S, Kim J-H, Hyun J-O, Chae HJ: Antimicrobial activity and cell cytotoxicity of Korean Plum-yem extract. J Appl Biol Chem. 2009, 52: 45-50. 10.3839/jabc.2009.009.CrossRef
22.
Watanabe K, Fukao T: Antibacterial effects of unripe Cephalotaxus harringtonia fruit extract on gram-positive bacteria. J Jpn Soc Food Sci Technol. 2009, 56: 533-540. 10.3136/nskkk.56.533.CrossRef
23.
Lamoral-Theys D, Pottier L, Dufrasne F, Nève J, Dubois J, Kornienko A, Kiss R, Ingrassia L: Natural polyphenols that display anticancer properties through inhibition of kinase activity. Curr Med Chem. 2010, 17: 812-825. 10.2174/092986710790712183.CrossRefPubMed
24.
Bortner CD, Oldenburg NBE, Cidlowski JA: The role of DNA fragmentation in apoptosis. Trends Cell Biol. 1995, 5: 21-26. 10.1016/S0962-8924(00)88932-1.CrossRefPubMed
25.
Yu J, Liu H, Lei J, Tan W, Hu X, Zou G: Antitumor activity of chloroform fraction of Scutellaria barbata and its active constituents. Phytother Res. 2007, 21: 817-822. 10.1002/ptr.2062.CrossRefPubMed
26.
Wolter F, Akoglu B, Clausnitzer A, Stein J: Downregulation of the cyclin D1/Cdk4 complex occurs during resveratrol-induced cell cycle arrest in colon cancer cell lines. J Nutr. 2001, 131: 2197-2203.PubMed
27.
Shankar S, Srivastava RK: Involvement of Bcl-2 family members, phosphatidylinositol 3'- kinase/AKT and mitochondrial p53 in curcumin (diferulolylmethane)-induced apoptosis in prostate cancer. Int J Oncol. 2007, 30: 905-918.PubMed
28.
Kim YA, Lee WH, Choi TH, Rhee SH, Park KY, Choi YH: Involvement of p21WAF1/CIP1, pRB, Bax and NF-kappaB in induction of growth arrest and apoptosis by resveratrol in human lung carcinoma A549 cells. Int J Oncol. 2003, 23: 1143-1149.PubMed
29.
Singleton VL, Rossi JA: Colorometry of total phenolics with phosphomolybdic-phosphotungstic acid reagents. Amer J Enol Viticult. 1965, 16: 144-158.
30.
Woisky RG, Salatino A: Analysis of propolis: Some parameters and procedures for chemical quality control. J Apicult Res. 1998, 37: 99-105.CrossRef
31.
Mensor LL, Menezes FS, Leitao GG, Reis AS, Santos TC, Coube CS, Leitao SG: Screening of Brazilian plant extracts for antioxidant activity by the use of DPPH free radical method. Phytother Res. 2001, 15: 127-130. 10.1002/ptr.687.CrossRefPubMed
32.
Duan X, Wu G, Jiang Y: Evaluation of anti oxidant properties of phenolics from lichi fruit in relation to pericarp browning prevention. Molecules. 2007, 12: 759-771. 10.3390/12040759.CrossRefPubMed
33.
Oyaizu M: Studies on products of the browning reaction. Antioxidative activities of browning reaction products prepared from glucosamine. Jpn J Nutr. 1986, 44: 307-314. 10.5264/eiyogakuzashi.44.307.CrossRef
34.
Bauer AW, Kirby WMM, Sherris JC, Turck M: Antibiotic susceptibility testing by a standardized single disk method. Am J Clin Pathol. 1966, 45: 493-496.PubMed
35.
Mosmann T: Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J Immunol Methods. 1983, 65: 55-63. 10.1016/0022-1759(83)90303-4.CrossRefPubMed
36.
Patel S, Kasoju N, Bora U, Goyal A: Structural analysis and biomedical applications of dextran produced by a new isolate Pediococcus pentosaceus screened from biodiversity hot spot Assam. Bioresource Technol. 2010, 101: 6852-6855. 10.1016/j.biortech.2010.03.063.CrossRef
37.
Riccardi C, Nicoletti I: Analysis of apoptosis by propidium iodide staining and flow cytometry. Nat Protoc. 2006, 1: 1458-1461. 10.1038/nprot.2006.238.CrossRefPubMed
38.
Fuke H, Shiraki K, Sugimoto K, Tanaka J, Beppu T, Yoneda K, Yamamoto N, Ito K, Masuya M, Takei Y: Jak inhibitor induces S phase cell-cycle arrest and augments TRAIL-induced apoptosis in human hepatocellular carcinoma cells. Biochem Biophys Res Commun. 2007, 363: 738-744. 10.1016/j.bbrc.2007.09.049.CrossRefPubMed
39.
Kwanchai AG, Arturo AG: Statistical procedures for agricultural research. 1984, Singapore: John Wiley and Sons, Inc
Metadata
Title
Antioxidant, antibacterial, cytotoxic, and apoptotic activity of stem bark extracts of Cephalotaxus griffithii Hook. f
Authors
Dinesh Singh Moirangthem
Narayan Chandra Talukdar
Naresh Kasoju
Utpal Bora
Publication date
01-12-2012
Publisher
BioMed Central
Published in
BMC Complementary Medicine and Therapies / Issue 1/2012
Electronic ISSN: 2662-7671
DOI
https://doi.org/10.1186/1472-6882-12-30

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