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BMC Endocrine Disorders

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Open Access 01-12-2019 | Hypothyroidism | Research article

The expression of sirtuins, superoxide dismutase, and lipid peroxidation status in peripheral blood from patients with diabetes and hypothyroidism

Authors: Abdullah Al-Khaldi, Samar Sultan

Published in: BMC Endocrine Disorders | Issue 1/2019

Abstract

Background

Sirtuin 1 (SIRT1) and sirtuin 3 (SIRT3) proteins have an important role in counteracting oxidative stress. Although diabetes and hypothyroidism (HT) are both characterized by oxidative stress, the mechanisms are not fully understood. This study investigated the effects of type 1 diabetes (T1D), type 2 diabetes (T2D), and HT on the expression levels of SIRT1, SIRT3, and manganese superoxide dismutase (SOD2).

Methods

Gene expression of SIRT1, SIRT3, and SOD2 was measured using real-time PCR. The protein expression of SOD2 and lipid peroxidation (thiobarbituric acid reactive substances) was measured by the TBARS Assay kit and enzyme-linked immunosorbent assay (ELISA) respectively.

Results

The results showed that the SIRT1 and SIRT3 levels were lower in peripheral blood samples from patients with T1D, T2D, or HT than in healthy individuals. Interestingly, the mRNA and protein expression levels of SOD2 were higher in all three patient groups. Lipid peroxidation was higher in the patients with HT than in the healthy individuals.

Conclusions

These results indicate alterations in the expression levels of sirtuins and superoxide dismutase in diabetes and HT, which may be related, at least in part, to the oxidative stress. Identifying such alterations in those patients will pave the way towards the development of drugs to enhance SIRT1 and SIRT3 expression and their activity to prevent the damaging effect of oxidative stress.

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Title: The expression of sirtuins, superoxide dismutase, and lipid peroxidation status in peripheral blood from patients with diabetes and hypothyroidism
Authors: Abdullah Al-Khaldi
Samar Sultan
Publication date: 01-12-2019
Publisher: BioMed Central
Keywords: Hypothyroidism
Type 1 Diabetes
Type 2 Diabetes
Published in: BMC Endocrine Disorders / Issue 1/2019
Electronic ISSN: 1472-6823
DOI: https://doi.org/10.1186/s12902-019-0350-y

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The expression of sirtuins, superoxide dismutase, and lipid peroxidation status in peripheral blood from patients with diabetes and hypothyroidism

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Background

Methods

Results

Conclusions

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